Team:Thessaloniki/Part Collection
Parts Collection
Parts Collection
We submitted a library of well-characterized parts that can be utilized by the synthetic biology community to render complex genetic networks more robust and able to maintain functionality over a wider range of conditions. Our toolbox consists of 2 mechanisms of promoter stabilization that achieve copy number-independent gene expression, each with its unique characteristics and potential applications. In addition, to allow for on the fly control of protein expression, we have introduced a theophylline riboswitch that controls the translation rate of the downstream Open Reading Frame.
| BioBrick ID | Description | USE |
| BBa_K2839012 | TALEsp1 Pupsp1 promoter with translational control | TALE Promoter Stabilization Inducible Expression |
| BBa_K2839017 | 12.1 Theophylline Riboswitch +sfGFP-luciferase fusion protein | Inducible Expression |
| BBa_K2839006 | Theo27 theophylline riboswitch controlling fused sfGFP expression | Inducible Expression |
| BBa_K2839000 | TALEsp1-Pupsp1 stabilized promoter | TALE Promoter Stabilization |
| BBa_K2839004 | Talesp1-Psp1w1 stabilized promoter (B0030) | TALE Promoter Stabilization |
| BBa_K2839005 | Talesp1-Psp1w1 stabilized promoter (B0032) | TALE Promoter Stabilization |
| BBa_K2839014 | TALEsp2-Pupsp2 stabilized promoter | TALE Promoter Stabilization |
| BBa_K2839008 | Insulated Psp1w1 promoter (B0030 RBS) | TALE Promoter Stabilization |
| BBa_K2839007 | Insulated Psp1w1 promoter (B0032 rbs) | TALE Promoter Stabilization |
| BBa_K2839016 | CRISPRi stabilized J23104 promoter | CRISPRi Promoter Stabilization |