Team:iTesla-SoundBio/RestrictionDigest

Double Digest with NEB Reagents

Reagent	50 uL rxn
RNase-free Water	Fill to 50 uL
10X NEBuffer*	5 uL
Template DNA	1 ug (1000 ng)
Enzyme 1	1 uL
Enzyme 2	1 uL

 

Incubate at 37 C for 1 hour and heat inactivated** for 20 min.

 

*Which buffer should be used depends on the enzymes and can be determined by the NEBcloner Digest Finder.

**Heat inactivation temperature depends on the enzymes and can be found here.