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			<li><a href="https://2018.igem.org/Team:OUC-China/Human_Practices">Human Practices</a></li>
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  <li ><a href="#tips1" id="thu" >1.1 SILVER-SAFETY</a></li>
  <li><a href="#tips2"  id="thu">1.2 SILVER-ESTABLISH LINKS BETWEEN PROJECT & STAKEHOLDERS</a></li>
  <li><a href="#tips3"  id="thu">1.3 SILVER-MEET UP</a></li>
  <li><a href="#tips4"  id="thu">2.1  INTRODUCTION</a></li>
  <li><a href="#tips5"  id="thu">2.2  INTEGRATION</a></li>
  <li><a href="#tips6"  id="thu">2.3 INTEGRATING PROJECT WITH A GAME "CSY4 GO!"</a></li>
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										<h2 class="major"><span>Human Practices</span></h2>
<p>


<h3><font size="4" color="#008B45">Achievements</font></h3>
<font size="2" color="black">
★We focused on biosafety and ensure project safety, lab work safety and did lots of biosafety educations.<br />
★We helped hold the 5th Conference of China iGEMers Community successfully and got lots of advice from other team in CCiC. <br />
★We integrated our project with labs and changed our project by applying our miniToe part into polycistron.<br />
★We expanded miniToe family according to the labs and verified by our model, making our system apply wider.<br />
★We formed a long-term relationship with biotech companies and got ideas about fundamental research application according to the market commands.<br />
★We designed a game named “Csy4 GO!” based on an enzyme that plays an important role in our project, transforming obscure biological terms into interesting pictures as well as stories. <br />
★ We did lots of <a href="https://2018.igem.org/Team:OUC-China/Public_Engagement">education and public engagement</a> work based on our project and integrated human practice achievements. <br /></font><a id="tips1"></a>
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/fc/T--OUC-China--%E7%BA%BF.png" width="900"></div>



<h3>Silver</h3>
<h4 ><font size="4" color="#8FBC8F">Safety</font></h4>
1.Biosafety considerations and investigation for our project
<br />Having identified our project, we discussed the biosafety of our project with professor Liang who has worked for years on nucleic acid detection and biosensor. We discussed with professor Liang on the safety and feasibility of our project . For the safety of our project, our experimental strain is <i>E.coli</i> DH5 Alpha, a very common strain in biological experiments. As long as we do experiments according to microbial and molecular experimental operation specification, there will be no safety issues. For the feasibility of our project, designing different structures of hairpin and enzyme to regulate the expression of the gene of interest is very useful for the future work. 
<br /><br />
<br />2.Biosafety Project 
<br />Because the track of our project is Foundational Advance, the downstream products have nothing but fluorescent proteins which act as a reporter to characterize our experimental results. What's more, nonpathogenic bacteria <i>E.coli</i> DH5 Alpha is employed as the main chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when using hairpin and Csy4 enzyme to tune the expression of genes, it is relatively safe for industrialized applications and scientific research.
In our project, all parts we have utilized are selected from Risk Group 1, none of which are with a Red Flag. As for microbial organisms, the microbial organisms have no chances to escape from our laboratory because we use ultraviolet sterilization after experiments. Furthermore, all equipment used for recombination strains will be sterilized by High-Pressure Steam Sterilization Pot. 
<br /><br />
<br />3.Biosafety lab work
<br />Our cautiousness about experiments makes it even impossible for classis to escape. Every week, we do a thorough laboratory cleaning, including spraying alcohol and disinfectant to sterilize. Every day we check all the instruments and strains in our lab. For the lack of nutrition, recombinant strains cannot survive outside the lab. What's more, the teacher responsible for laboratory safety checks our safety work every week. And we are certain that everything we did is under our control.
<br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/88/T--OUC-China--hp01.jpg" width="600"></div> 	
<div align="center"><p>Fig.1 We have strict safety regulations and safe operation.</p></div> 
					
4. Biosafety education
<br />We organized a summer camp to popularize life science with middle school students, and teach them basic microbial experimental operations such as butterfly specimens, microbial painting and plasmid extraction. More importantly, we explained the safety of laboratories to them before entering the laboratory in order to raise their biosafety awareness. No experiment carries zero risk to the experimenters, so we must learn how to protect ourselves. Before each experiment, they were educated to be equipped with some necessary facilities, such as latex gloves, nitrile gloves, goggles and lab coats to protect them from biotic and abiotic hazards. Undoubtedly, safety is always in the first place for us. 
<br /><a href="https://2018.igem.org/Team:OUC-China/Safety">See more details in our Safety</a><br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/fe/T--OUC-China--goujibadong.jpg" width="600"></div> 	<a id="tips2"></a>
<div align="center"><p>Fig.2 We explained the safety of laboratories to high school students</p></div> 								<h4 ><font size="4" color="#8FBC8F">Establish links between project and stakeholders</font></h4>
1. Integration with labs
<br />The purpose of our project is to regulate the expression of a target gene with the help of miniToe structure which is composed of endoribonuclease Csy4 and hairpins. Initially, we just wanted to realize the regulation of one gene expression. By establishing dialogues with labs, we found the limitations of our thoughts. Thankfully, enlightened by Professor Liang in Ocean university of China and Professor Du in Institiue of microbiology, we changed our initial thoughts and extended the expression gradients of target gene by model prediction and mutation of hairpins and Csy4. What's more, we applied our minToe to polycistron with the help of modelling for further application. 
<br /><font color="#8FBC8F"><a href="#lalala">See more details in our Gold & Integrated human practice</a></font>	<br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/20/T--OUC-China--hp03.jpg" width="600"></div> 	
<div align="center"><p>Fig.3 We communicated with Professor Du about our project. </p></div> 
<br />2. Integration with biotech companies
<br />The best way to further verify the practical application of our project is to investigate in the biological companies who are good at transforming basic researches into practical results . We visited some relevant local biotech companies such as BGI, Qingdao Bright Moon Seaweed Group Co.LTD and Qingdao youdu biotechnology Co.LTD. We introduced our project to the stakeholders and also gathered many good suggestions. Importantly, they applauded our ideas and proposed some application of our project on production. Enlightened by the investigation, we ensured our ideas of applying our system to polycistron expression system and put it into practice. 
<br /><font color="#8FBC8F"><a href="#lalala">See more details in our Gold & Integrated human practice</a></font><br /><br />
	<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/25/T--OUC-China--hp05.jpg" width="600"></div> 	
<div align="center"><p>Fig.4 We went to biotech companies and it really inspired us.</p></div> 								3. Integration with other iGEM teams
<br />In CCiC(Conference of China iGEMers Community), we communicated with other teams about our project.  XJTU-China came to exchange their ideas with us, and the similarity between the two project made us collaborated. After finishing the experiments of polycistron system, we integrated the system with their project and built a model for them. In the process of human practice, we established a platform with stakeholders for a long term cooperation and feedback. Our project is a great example of fundamental research being transformed into application. So we shared our experience with Tianjin, ZJU-China, NUDT-China team and documented for future iGEMers.<a id="tips3"></a>
<br /><font color="#8FBC8F"><a href="#lalala">See more details in our Gold & Integrated human practice</a></font>
<br /><a href="https://static.igem.org/mediawiki/2018/3/3e/T--OUC-China--fundamental_research_report.pdf	">Click here to download our report.</a>
<br /><br /><h4 ><font size="4" color="#8FBC8F">Meet up</font></h4>											
1. Co-organizer of CCiC, Conference of China iGEMers Community
<br />Served as a co-organizer, OUC-China tried best and helped the organizer <a href="https://2018.igem.org/Team:ShanghaiTech/Collaborations">Shanghaitech</a> hold the conference successfully. For the conference preparation, We were responsible for contacting each team, sending a questionnaire to them and investigating the commands of each team for the meeting. Then we organized and summarized all information of 62 teams. At each key time, we reminded each team to submit project summary, team logo and then sorted out according to the track. As an old team, OUC-China actively participated in After iGEM activities and shared our team heritage and experience with other teams.
<br /><br />As a part of the joint iGEM session, OUC-China joined the CCiC Executive Committee and worked with the members of the Executive Committee, organizers, sponsors to plan the conference and contribute to the CCiC heritage.
			
<br /><br />										
	<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/fb/T--OUC-China--hp06.jpg" width="600"></div> 
<div align="center"><p>Fig.5 Opening ceremony of the 5th CCiC.</p></div> 

	<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/f5/T--OUC-China--hp07.jpg" width="600"></div> 
<div align="center"><p>Fig.6 We communicated with other teams in CCiC.</p></div> 
									
	<div align="center"><img src="https://static.igem.org/mediawiki/2018/0/0a/T--OUC-China--hp08.jpg" width="600"></div> 	
<div align="center"><p>Fig.7 As the co-organizer of the 5th CCiC, we designed a handbook for CCiC.</p></div> 											
</p><a id="tips4"></a>
								
<p>
<h4 ><font size="1" color="white"><a id="lalala">CYJSSB</a></font></h4>
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/fc/T--OUC-China--%E7%BA%BF.png" width="900"></div> 

<h3>Gold & integrated</h3>
<h4 ><font size="4" color="#8FBC8F">1. Introduction</font></h4>
<i>"How do fundamental research results be transformed into applications that benefit human?" "How do you apply social feedback to a project?" "How do you turn an obscure basic research into an easy-to-understand form?" There are many questions waiting to be solved by OUC-China. </i>
<br /><br />Through communication, we found that many other teams were also confused by these problems. In order to transform the basic research results into the practical applications, we communicated with many stakeholders, visited related biotech companies, and asked professors in related fields. Soon, an inspiration came into our mind, and we created a game based on our project in order to popularize the biology science to more people. 
<br /><br />In the process of spreading our project, it is difficult for many amateurish people to understand these principles, especially when we mentioned some biological terms, such as "CRISPR". With the help of our game and the game brochure, the obscure principle became vivid and interesting to them, which was really a good feedback for us. Apart from that, we teamed up with five other iGEM teams to set up a Basic Research Results Transformation Team, in order to jointly research on local biotech companies and explore a fixed pattern for the basic research transformation, hoping to give a reference to the future iGEM teams. In fact, we have done a lot of work about <a href="https://2018.igem.org/Team:OUC-China/Public_Engagement">education and public engagement</a> based on our project, such as our game, biotech companies research report and so on, which is a part of our human practice.  <br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/d/dc/T--OUC-China--HP-timeline.jpg
" width="900"></div> <a id="tips5"></a>

	<br /><br /><h4 ><font size="4" color="#8FBC8F">2. Integration</font></h4>	
By establishing models and molecular predictions, OUC-China constructed a toolkit and named it MINITOE FAMILY. The toolkit can regulate the expression level of targeted gene in a gradient form. 

<br /><br />2.1 Integration with labs

<br />"Your hairpin mutant is very conservative. Only a pair of base mutation will not achieve your idealized effect. You can try more bases modifications." Professor Liang, who studies the range of mutations nucleic acids for about twenty years, gave us some advice on the hairpin mutation.
<br /><br />Having accepted his suggestion, we expanded the range of base mutations, from a pair of base mutation to two or more pair of bases mutation, from the bases mutation on the stem to the bases mutation on the loop. Indeed! Expanding the range of mutations showed us a better expanded expression gradients, which formed a useful toolkit "miniToe family" by combining different hairpins with Csy4 mutations. Initially, professor Liang doubted whether crRBS and RBS can be separated after the interaction with Csy4. He was concerned that the cleavage of Csy4 and steric hindrance was not sufficient to break the hydrogen bond between base pairs. But his concern was broken by the expression of fluorescent gene. <br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/7/79/T--OUC-China--hp008.jpg" width="600"></div> 	
<div align="center"><p>Fig.8 We expanded the range of mutations to get a better expanded expression gradients.</p></div> 	<i>"A great idea! It will be better if you can regulate the expression ratio of more than one gene. So your application range can be wider." said Professor Du of the Institute of Microbiology, Chinese Academy of Sciences.</i>           
<br /><br />Du ever changed the intensity of RBS to achieve different expression levels of the target gene. So we went to Beijing and consulted some question of our project with him. He gave an example of our project application. The traditional way is to knock out a gene then observe different performance of the organism, and your experimental results can be used to explore the effect of different gene expression levels on the function of organisms. Indeed! Inspired by Teacher Du, we considered applying the MINITOE structure to the regulation of gene expression in polycistron expression systems.<br />	<br />											
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/fc/T--OUC-China--hp11.jpg" width="600"></div> 	
<div align="center"><p>Fig.9 Professor Du helped us analyze our project in Institute of Microbiology.</p></div> 
<div align="center"><img src="https://static.igem.org/mediawiki/2018/1/10/T--OUC-China--hp12.jpg" width="600"></div> 	
<div align="center"><p>Fig.10 Du said, "Your project can be used to explore the effect of different gene expression levels on the function of organisms"</p></div> 								
<div align="center"><img src="https://static.igem.org/mediawiki/2018/5/58/T--OUC-China--hp13.jpg" width="600"></div> 	
<div align="center"><p>Fig.11 We were enlightened by Professor Du and applied our miniToe to polycistron</p></div> 
		
<br />	2.2 Integration with biotechnology companies and research institutes	
<br />Given that all basic researches aim at application and benefiting for human beings, we supposed that visiting the biotechnology companies to investigate the prospect of our project is a great idea. We went to the BGI, Qingdao Youdu Biotechnology Co.LTD and Qingdao Bright Moon Seaweed Group Co.LTD to integrate our project with social reality and communicate with technical staff in these companies. 
<br /><br />In this way, we learned about the current social needs and improved our project accordingly. At the same time, we established a long-term relationship with them. On the one hand, they can provide us an effective way of converting foundational research to actual application, on the other hand, we will inform them of the latest results in the lab, by which we can promote each other and make progress together.Fially, we form a research <ahref="https://static.igem.org/mediawiki/2018/3/3e/T--OUC-China--fundamental_research_report.pdf">report</a> about our experience.

<br /><br />2.2.1 BGI
<br />Firstly, we visited BGI research institute. BGI is the world's largest genomics research institute that applies scientific research results in the fields of medical health, agricultural breeding and resource conservation. It is committed to promoting the transformation of genetic science and technology, so as to bring benefits to human. We introduced our project to BGI researchers and visited the company's laboratory and breeding area. 
<br /><br />The researchers in the gene editing laboratory evaluated our project and proposed new application directions for our project, suggesting that we can apply the genetic circuit to the anabolic design of engineered bacteria and combine it with some industries such as fermentation engineering. At the same time, they told us that their lab was also trying to apply gene-editing techniques to the genetic breeding of Marine economic fish, which may also provide us with some ideas. We are very grateful to BIG for their valuable advice and they also encouraged us to combine our project with their labs. We will keep in touch with BGI in the future.<br /><br />							
<div align="center"><img src="https://static.igem.org/mediawiki/2018/3/3d/T--OUC-China--hp14.jpg" width="600"></div>
<div align="center"><p>Fig.12 BGI introduced their latest production to us.</p></div> 	
<div align="center"><img src="https://static.igem.org/mediawiki/2018/9/9f/T--OUC-China--hp15.jpg" width="600"></div>  	
<div align="center"><p>Fig.13 BGI gave us some advice on our project and it really enlightened us.</p></div> 			

<br /><br />2.2.2 Marine Institute, Chinese Academy of Sciences
<br />Inspired by BGI researchers, we arrived at the Marine animal molecular developmental biology and evolution laboratory of the institute of oceanology, Chinese academy of sciences. The main research direction of the laboratory is the development of gene resources and germplasm improvement of mariculture fish, which provides breeding materials and new breeding methods for the breeding of improved species of blue agricultural mariculture animals. Under the guidance of the researchers, we visited the laboratory and learned about the history of the laboratory as well as the results of the innovative research on Marine fish germplasm. 
<br /><br />We asked the teacher if our project could be combined with genetic breeding, she gave us some suggestions. At present, they use traditional genetic technology in model organism zebrafish to explore the function of target gene. We wondered if our project can be applied to their labs. The researchers said we could have a try and it is an innovation to apply our system to another model organism. Indeed! Applying our system into another model organism is an opportunity as well as a challenge. 
<br /><br />The researchers also encouraged us to combine our fundamental research with industry, which was an affirmation of our project. They are willing to apply our projects to their labs in the future.<br />	<br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/5/59/T--OUC-China--hp16.jpg" width="600"></div> 
<div align="center"><p>Fig.14 We went to the aquaculture bases of Marine Institute.</p></div> 
<div align="center"><img src="https://static.igem.org/mediawiki/2018/e/e7/T--OUC-China--hp17.jpg" width="600"></div> 	
<div align="center"><p>Fig.15 We went to Marine Institute and communicated with researchers.</p></div> 
					
<br />2.2.3 Qingdao Bright Moon Seaweed Group Co. LTD.
<br />In order to find more ideas, we came to Qingdao Bright Seaweed Group Co. LTD. It is a leading enterprise in the Marine biological industry of Shandong province, and the largest seaweed biological products enterprise in the world. We visited the seaweed science museum and the state key laboratory of algae active substances, learned about the alginate and seaweed polysaccharide industries. 
<br /><br />In the process, we came up with a new idea that we can use our system to regulate the expression of one or two enzymes that are needed for the processing and production. The company's research and development staff agreed with our idea and suggested that we should design a complete biological treatment scheme and explore its cultivation conditions to realize the better integration of our system with industry. We learned a lot from the journey of Qingdao Bright Moon Seaweed Group Co. LTD, which was a great progress for us.	<br />	<br />	
<div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8a/T--OUC-China--hp18.jpg" width="600"></div> 
<div align="center"><p>Fig.16 We went to the labs of Qingdao Bright Moon Seaweed Group Co.LTD and learned about their current researches.</p></div> 
<div align="center"><img src="https://static.igem.org/mediawiki/2018/1/1f/T--OUC-China--hp19.jpg" width="600"></div> 	
<div align="center"><p>Fig.17 We formed a long-term cooperation relationship with Qingdao Bright Moon Seaweed Group Co.LTD.</p></div> 								

<br />2.2.4 Qingdao Youdu Biotechnology Co.LTD.
<br />Youdu Biotechnology Co.LTD is mainly involved in the beauty industry, which has many production lines and beauty brands, which is a new and dynamic biological enterprise. We visited its factory, laboratory and production line of the enterprise, and preliminarily knew about the material selection, physical and chemical experiment and downstream product operation. We introduced our project to the researchers in the laboratory, who gave us a high evaluation of the project and affirmed our idea of applying the system to the regulation of anabolic circuits in order to integrate with the real industries better.
<br /><br />Since engaging in the beauty industry, they suggested that we can not only use our system to produce the enzymes that are needed to process raw material, but also directly produce some ingredients, which can be added to skin care products. And our polycistron system can even attempt to mix two materials proportionally in production. In addition to using E·coli as our engineering cells, we may also be able to apply the system to other chassis, such as yeast, to explore the most convenient way on factory production. We will also keep in touch with Youdu for a long term and hope that our system can really achieve industrial integration.<br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--OUC-China--hp20.jpg" width="600"></div> 	
<div align="center"><p>Fig.18 Qingdao Youdu Biotechnology Co.LTD applauded our polycistron system.</p></div> 

In the process of interaction with biotech companies, we gained a lot and integrated it with our project. We organized our biotech companies research reports into documents and shared our experience with other iGEM teams. Known that our project is about basic research, Tianjin University contacted us and we set up a fundamental research results transformation team together with several Chinese iGEM teams, Tianjin, ZJU, NUDT. We were glad to share our experience with them. 
Finally, we have finished <a href="https://static.igem.org/mediawiki/2018/3/3e/T--OUC-China--fundamental_research_report.pdf">a report</a> on the transformation of basic research results, hoping future iGEM teams can learn something from it.<a id="tips6"></a>
<br /><br />
<br /><h4 ><font size="4" color="#8FBC8F">3. INTEGRATING PROJECT WITH A GAME——< Csy4 GO!></font></h4>

<i>"The principles of all fundamental scientific research are obscure for amateurish people."</i>
<br /><i>"The form of the game < Csy4 GO!> is interesting!"</i>
<br />
<br />In the process of human practice, we tried to explain our projects to more people. But we found that they felt it difficult to understand, especially some amateurish people. In order to let more people understand the principles of biology in an interesting and effective way, we spent lots of time designing a game - < Csy4 GO!>, which is a good combination with our project. 
							
<br /><br />3.1 The game introduction<br />									
The leading role of < Csy4 GO!> is a little cute enzyme named Csy4 which is the main target of our project. Naturally, Csy4 is very active and able to protect the host from being harmed. We enumerate the functions of Csy4 from six parts--Immune, Secretion, Transport, Constitute, Recognition and Catalyze. Our game < Csy4 GO!> is based on these six parts. When we start the game, there will be six situations for players to choose, each situation includes one interesting story.<br /><br />
<div align="center"><video muted autoplay loop  width="500" ><source src="https://static.igem.org/mediawiki/2018/5/55/T--OUC-China--llll.mp4" ></video></div>
<br /><a href="https://2018.igem.org/File:T--OUC-China--Csy4_GO!.zip">You can download our windows version game.</a><br /><br />
Then the players can control Csy4 to cleave RNA chain in the right order and right place. The player's operation simulates the process of Csy4 protecting host from external damages. In that way players can not only enjoy our game < Csy4 GO!> but also learn about some functions of Csy4. Once the game starting, RNA chain will roll with the background at a certain speed, so the players should move fast enough to make sure Csy4 won't be touched by the top of screen.<br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/d/d2/T--OUC-China--hp21.jpg" width="400"></div> 	
<div align="center"><p>Fig.19 The logo of < Csy4 GO!>. </p></div> 								
<br /><br />3.2 Purpose of our game<br />							
As is known to us, the mechanism of endonuclease Csy4 in microorganisms is difficult to observe with our eyes straightly. But now < Csy4 GO!> solves this problem successfully. The game presents a microscopic view of the world with a more vivid picture. In this way, we can tell the story of Csy4 as well as the CRIPSR system to more amateurish people, thus achieving the goals of propagandizing our project and synthetic biology.
<br /><br />3.3 The designing process of our game<br />	
<br />	3.3.1 The game started:
<br />At first, we planned two game blueprints. The first is to control Csy4 to specifically cut RNA, which can explain the principle of Csy4. To make the game more interesting, we created the second one. The second is to control Csy4 moving on the RNA chain. To get the destination, they need to cleave on the certain site and keep falling.	<br />				
	<div align="center"><img src="https://static.igem.org/mediawiki/2018/7/7d/T--OUC-China--hp22.jpg" width="600"></div> 	
<div align="center"><p>Fig.20 The background of < Csy4 GO!>. </p></div> 								
<br />Having discussed in our team, we created the general design of our game. Given that no professional programmer in our team can take charge of this work, we asked Mingjie Shen for help, who has the experiences of programming mini-games . 
In our first blueprint, we characterized Csy4 the ability of cleaving the RNA by recognizing certain three base sites instead of cleaving RNA by recognizing the hairpin.
<br /><br />
Once the game started, there will be some golden coins suspending on the screen, and several kinds of RNA structures which are shown in pictures and rolling at the bottom. We hope that players can put the RNA structures on the proper position, use the certain keys to cleave the RNA and fall down, gather the golden coins and arrive the destination eventually.
<br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/5/5d/T--OUC-China--hp26.jpg" width="600"></div> 	
<div align="center"><p>Fig.21 Some small icons that we designed in our game.
</p></div> 

Many different cleave sites random are generated from four different kinds of ribonucleotide. At the beginning, Csy4 only have the ability to recognize and cleave several sites. Players should get the ability to cleave at new site by passing more game levels or buying in the store using the money gathering during game.
<br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/3/3b/T--OUC-China--hp23.jpg" width="600"></div> 	
<div align="center"><p>Fig.22 Six kinds of RNA with specific structures.</p></div> 
<This is our initial design about the first blueprint.
<br />In this part, we enact the game rules: 
<br />a. Csy4 cleaves the RNA by certain cut site instead of special structure. 
<br />b. Csy4 starts at one side of the screen, it's supposed to go the diagonal side.
<br />c. Players should use RNA to help Csy4 reach the end.
<br />d. The golden coins gathered during the game can be used to buy more skills (active cleave sites) to cut the RNA.
<br />e. There will be several levels,and every time you pass a level you can get a new ability.
<br />After discussing with the computer games designer Mingjie Shen, we started our design!
<br />
<br />3.3.2 The first step of our game:
<br />During programming, we designed 6 RNA with different structures carrying one or two sites on it. Players can choose the certain sites and cut it to help Csy4 fall. And Csy4 is designed as a little cute white devil in the game.<br />
<br />The game was divided into 6 parts on the basis of the properties of proteins: Immune, Secretion, Transport, Constitute, Recognition, Catalyze. The difficulties among those parts from Immune to Catalyze is in gradient.<br />
<br />We also designed many other widgets we need in the game.
<br />In this part, we've done: 
<br />a. Designed the 6 kinds of RNA with specific structures.
<br />b. Designed the cartoon character of Csy4.
<br />c. Designed the gradient difficulties in game.
<br />d. Designed other widgets in game.	<br /><br />
									
<div align="center"><img src="https://static.igem.org/mediawiki/2018/5/5a/T--OUC-China--hp24.jpg" width="600">
</div> 	
<div align="center"><p>Fig.23 The cartoon character of Csy4. </p></div> 									
									
<br />3.3.3 Meet some troubles
<br />While programming, we found that the RNA rolling sidebar is hard to realize. And it's difficult for most players to control the movement of Csy4 and arrange the certain RNA at the same time. So we modified the game rules -- delete the rolling sidebar and immobilize RNA to lengthen the path and make the rolling background picture come true. <br />
<br />In our original vision, Csy4 can only move horizontally. It can not jump or fall down at both ends of the RNA. Refer to the RNA picture we showed in "The first step of our game", which is difficult to program such a flexible path, we redesigned RNA and made Csy4 jump.
<br /><br />
In this part, we've done:
<br />a. Modified the RNA structure.
<br />b. Modified game rules:
<br />c. Deleted the rolling sidebar.
<br />d. Rolled the background picture.<br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/3/35/T--OUC-China--hp25.gif" width="600"></div> 	
<div align="center"><p>Fig.24 The dynamic effects of our role. </p></div> 
								
<br />3.3.4 The game rudiment
<br />We've finished most of programming in this part. When testing, it works well. We finally decided to name it < Csy4 GO!>. Although the shop part of the game is still missing (including the gold coins that expect to be shown on the screen), and the game levels are not in the gradient difficulty. In order to make the game better integrated with biological elements, we took the prototype of biological environment as background picture. 
<br />
<br />
<div align="center">

<video muted autoplay loop  width="500" ><source src="https://static.igem.org/mediawiki/2018/6/61/T--OUC-China--Videos_about_this_game_%282%29.mp4" ></video></div>
<div align="center"> <video muted autoplay loop  width="500" ><source src="https://static.igem.org/mediawiki/2018/c/cd/T--OUC-China--FAIL.MP4" ></video></div>	

<div align="center">Some productions in this game .</div> 	
<br />In this part, we've done:
<br />a. Named the game.
<br />b. Finished most of the programming except the shop and the gradient difficulty game levels parts.
<br />c. Designed background picture of the game.
<br />d. Users can operate the game smoothly.
<div id="accordion">
<h3>Click here to see our background picture in the game</h3>
  <div>
    <p>
<li><div align="center"><img src="https://static.igem.org/mediawiki/2018/5/57/T--OUC-China--hp100.jpg" width="600"> </div></li><br />
<li><div align="center">Fig.25 We took the prototype of biological environment as the background picture of our game design.
</div></li>
  </p>
  </div>
  </div>
<br /><div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c7/T--OUC-China--cgxgt.gif" width="500"></div> 
<div align="center"><p>If you win the game, Csy4 will cheer up and say "Congratulations!" </p></div> 
<div align="center"><img src="https://static.igem.org/mediawiki/2018/a/ad/T--OUC-China--sbxgt.gif" width="500"></div> 	
<div align="center"><p>If you lose the game, Csy4 will be disappointed. </p></div> 

<br /><br />3.4 A creative comic book brochure about our game < Csy4 GO!><br />
<br />To make people have a better understanding of both the game and our project, we designed six comic pictures to represent the six game levels separately. The picture will be shown before the game starts to help players understand situations clearly. The comic pictures not only help in driving the storyline but also enhance the enjoyment of the game. They are so cute and straightaway that can be really helpful in engaging the audience.
<br /><br />
There are the pictures and the story in six game levels:
<br />①Immune: when the virus invade our bodies, the cells are on the verge of death or destruction. Consequentially, the cells have their own ways like producing certain protein to resist the virus. However, when the cells can't bear the heavy load, we can ask Csy4 for help by cutting certain mRNA to improve the protein army.
<br /><br />			
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/f4/T--OUC-China--hp27.jpg" width="700"></div> 	
<div align="center">Immune </div> <br /><br />								
②Secretion: our body is constituted by thousands of cells, and those cells don't live alone but communicate with each other closely through secretting unique proteins. When we want to construct the connect between two cells, we can use Csy4 to create certain secretion protein and help them send the messages to each others.
<br /><br />								
<div align="center"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--OUC-China--hp28.jpg" width="700"></div> 	
<div align="center"> Secretion </div> <br /><br />								
③Transport: Hey! Have you seen the huge one lying there with a bag of potato chips? It is always eating, eating, eating…… It's so fat that the normal size carrier is not big enough for it. It seems like we have to Built a carrier for it professionally. And Csy4 can be a help. 
<br /><br />								
<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/2e/T--OUC-China--hp29.jpg" width="700"></div> 	
<div align="center">Transport </div> <br /><br />	
④Constitute: Warning! Warning! Cellular components are destroyed! ……In the short, precise life cycle, cells just like a space ship waving in the universal but have their certain destination, It's normal for it to fix and replace the damaged components, but when the cells are given the task that its predecessors never had, which means there're no certain methods can be find to solve the problems. At that time, Csy4 can be helpful. 
<br /><br />
<div align="center"><img src="https://static.igem.org/mediawiki/2018/9/92/T--OUC-China--hp30.jpg" width="700"></div> 	
<div align="center">Constitute </div> <br /><br />							
⑤Recognition: Some cells are nothing special but actually have the vitally important task. One day, Clark Kent walks down the street as usual, doesn't recognize the world waiting for him to save. He has no response to cosmic signals…… Csy4 can be helpful, let's change the signals that make it easy to understand. Well, it seems we did it. 
<br /><br />							
<div align="center"><img src="https://static.igem.org/mediawiki/2018/9/98/T--OUC-China--hp31.jpg" width="700"></div> 	
<div align="center">Recognition </div> <br /><br />							
⑥Catalyze: Enzymatic reactions are common, one of the most important in them is the metabolism of energy, our bodies’ move, cellular activities and so on rely on it. Sometimes, It's necessary to enhance the reaction to provide more energy. Here, we can ask Csy4 for help.
<br /><br />	
<div align="center"><img src="https://static.igem.org/mediawiki/2018/5/55/T--OUC-China--hp32.jpg" width="700"></div> 	
<div align="center">Catalyze </div> <br /><br />								

<br />The game manual contents 3 parts -- abstract of < Csy4 GO!>, abstract of Csy4 and story and pictures of comic. Manual was designed to look like an envelope.
<br />Our creative design of comic picture:
<br />	
	<div align="center"><img src="https://static.igem.org/mediawiki/2018/7/7a/T--OUC-China--edu13.jpg" width="600"><br />	
<img src="https://static.igem.org/mediawiki/2018/f/f9/T--OUC-China--edu131.jpg" width="600"></div> 
<div align="center"><p> Our creative game manaul</p></div>	 

<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/23/T--OUC-China--game_manaul.jpg" width="600"></div> 
<div align="center"><p> The interesting way of reading the game Brochure "Csy4 Go!"</p></div>								
<br />
<br /><br />

<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/fc/T--OUC-China--%E7%BA%BF.png" width="900"></div>
<h3><font size="4" color="#008B45">Achievements</font></h3>
<font size="2" color="black">
★We focused on biosafety and ensure project safety, lab work safety and did lots of biosafety educations.<br />
★We helped hold the 5th Conference of China iGEMers Community successfully and got lots of advice from other team in CCiC. <br />
★We integrated our project with labs and changed our project by applying our miniToe part into polycistron.<br />
★We expanded miniToe family according to the labs and verified by our model, making our system apply wider.<br />
★We formed a long-term relationship with biotech companies and got ideas about fundamental research application according to the market commands.<br />
★We designed a game named “Csy4 GO!” based on an enzyme that plays an important role in our project, transforming obscure biological terms into interesting pictures as well as stories. <br />
★ We did lots of <a href="https://2018.igem.org/Team:OUC-China/Public_Engagement">education and public engagement</a> work based on our project and integrated human practice achievements. <br /></font>
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