Difference between revisions of "Team:iTesla-SoundBio/Project/Interlab"
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| − | <div class="headers"><p><b><u>Summary</u>:</b> For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration. <br> Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers. Below, is the timeline that we followed in completing the InterLab protocol.</p></div> | + | <div class="headers"><p><b><u>Summary</u>:</b> For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration. <br> Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers. </P> |
| + | <p>Below, is the timeline that we followed in completing the InterLab protocol.</p></div> | ||
<img src="https://static.igem.org/mediawiki/2018/5/55/T--iTesla-SoundBio--interlabtimeline.jpg" alt= "ludoxprotocol" style="width:80%" align= "middle"> | <img src="https://static.igem.org/mediawiki/2018/5/55/T--iTesla-SoundBio--interlabtimeline.jpg" alt= "ludoxprotocol" style="width:80%" align= "middle"> | ||
| + | <div class="horizontalline"> | ||
| + | </div> | ||
| − | < | + | <div class="middles"><h1>Part One- LUDOX Calibration Protocol</h1></div> |
| − | <h2>96 Well Plate Table</h2> | + | <div class="middles"><h2>96 Well Plate Table</h2></div> |
<table align="center"> | <table align="center"> | ||
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<p>Here are the results for the Absorbance 600 of the LUDOX protcol</p> | <p>Here are the results for the Absorbance 600 of the LUDOX protcol</p> | ||
| + | <h2> OD600 Reference Point Data </h2> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/8/8b/T--iTesla-SoundBio--LUDOX_absorbance_readings.jpeg.JPG" alt= "ludoxprotocol"> | ||
| − | |||
| + | <div class="horizontalline"> | ||
| + | </div> | ||
| − | |||
| + | <div class="middles"> | ||
| + | <h1> Part Two- Microsphere Protocol</h1> | ||
| + | </div> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/f/f6/T--iTesla-SoundBio--microspherestandardcurvelinear.jpg" alt= "microspherecurve", style="width:40%;"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/6/64/T--iTesla-SoundBio--microspherestandardcurvelog.jpg" alt= "microsphere log curve", style="width:40%;"> |
| − | |||
| + | <div class="horizontalline"> | ||
| + | </div> | ||
| − | |||
| − | < | + | <div class="middles"><h1> Part Three- Fluorescein Protocol</h1><div> |
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/4/44/T--iTesla-SoundBio--fluoresceinlinear.jpg" alt= "fluoresciencurve", style="width:40%;"> |
| + | <img src="https://static.igem.org/mediawiki/2018/5/51/T--iTesla-SoundBio--fluorescein.jpg" alt= "microsphere log curve", style="width:40%;"> | ||
| − | < | + | |
| + | |||
| + | <div class="horizontalline"> | ||
| + | </div> | ||
| + | |||
| + | |||
| + | <div class="middle"> | ||
| + | <h1>Bronze Medal Criterion #4</h1> | ||
| + | </div> | ||
| + | |||
| + | <div class="middles"> | ||
<p> | <p> | ||
| − | <b>Standard Tracks:</b> <br> | + | <b>Standard Tracks:</b> |
| + | <br> | ||
Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range. | Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range. | ||
| + | <br> | ||
| + | For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab"> InterLab study </a>, all work must be shown on this page. | ||
</p> | </p> | ||
| − | + | </div> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | </ | + | |
| − | + | ||
<img src="https://static.igem.org/mediawiki/2018/f/fe/T--iTesla-SoundBio--interlabjoy.jpg" alt= "interlab joy", style="width:80%;"> | <img src="https://static.igem.org/mediawiki/2018/f/fe/T--iTesla-SoundBio--interlabjoy.jpg" alt= "interlab joy", style="width:80%;"> | ||
Latest revision as of 05:37, 1 September 2018
Summary: For the 2018 InterLab study, high school students at iTesla-SoundBio prepared various colonies and dilutions to test for trends absorbance, fluorescence, and concentration.
Massive thanks to the Washington iGEM InterLab team for guiding us through the protocol and lending us their plate readers.
Below, is the timeline that we followed in completing the InterLab protocol.
Part One- LUDOX Calibration Protocol
96 Well Plate Table
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | LUDOX | ddH2O | ||||||||||
| B | LUDOX | ddH2O | ||||||||||
| C | LUDOX | ddH2O | ||||||||||
| D | LUDOX | ddH2O | ||||||||||
| E | ||||||||||||
| F | ||||||||||||
| G | ||||||||||||
| H |
Here are the results for the Absorbance 600 of the LUDOX protcol
OD600 Reference Point Data
Part Two- Microsphere Protocol
Part Three- Fluorescein Protocol
Bronze Medal Criterion #4
Standard Tracks:
Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
For teams participating in the InterLab study , all work must be shown on this page.
