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Revision as of 19:22, 2 September 2018
A CRISPR/Cas Base-Editing Toolkit
From Genome to Transcriptome, From Detection to Modification.
A Continuation and Beyond.
CRISPR/Cas9 DNA Base Editor
With the successful truncated dCas9-mediated transcriptional activation last year, we moved further this year into DNA base editing using CRISPR/Cas9 system. We also improved our truncated dCas9 further with novel strategies for higher binding efficiency.
Human-Centred Research
Our research involves not only the students and scientists, but also the Singapore public. While doing our project, we looked into the public opinions towards gene editing. Our human practice tells how our project evolved around the local community that we live in.
Adapt to Change.
CRISPR/Cas13b RNA Base Editor
As we have learnt through our interviews with medical doctors and interaction with the public, it is evident that RNA editing is more promising and better accep-ted in our society. Hence, we reflected on our project and ventured into RNA editing.
Aim Even Higher.
Modification-Sensitive Nanopore Sequencing
To address the significant concern around the safety of RNA editing technology, we designed an unbiased, accurate RNA sequencing tool. Expanding the nanopore sequencing platform, we hope to enhance the saftey of RNA editing.