Difference between revisions of "Team:NUS Singapore-A"
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| − | < | + | <h2 id='Overview'>Coup Dy'état: Eco-friendly Bio-manufacturing of Flavonoid Dyes in Escherichia coli via Computer-mediated Optogenetic Regulation</h2> |
<p style="margin: 0 5%"> Synthetic clothing dyes are a major source of water pollution. Natural dyes are more beneficial to the environment, but production is land-intensive and low-yield. To increase the viability of natural dyes as an alternative to synthetic dyes, we developed a novel, eco-friendly method for the biomanufacturing of a natural flavonoid dye, luteolin, in E. coli. We performed mutagenesis for more efficient xylose metabolization, allowing us to use lignocellulosic waste as feedstock. Also, we designed an automated closed-loop cell-computer interface to enhance production through dynamic regulation and stress regulation. The system obtains feedback from an optical density sensor and a stress-sensing fluorescent reporter. As we control gene expression with a blue light repressible promoter, blue LEDs activate and deactivate accordingly to maintain an optimal rate of production, eliminating the need for chemical inducers. We envision that our system has great potential for finer control and optimization as more functions are incorporated. | <p style="margin: 0 5%"> Synthetic clothing dyes are a major source of water pollution. Natural dyes are more beneficial to the environment, but production is land-intensive and low-yield. To increase the viability of natural dyes as an alternative to synthetic dyes, we developed a novel, eco-friendly method for the biomanufacturing of a natural flavonoid dye, luteolin, in E. coli. We performed mutagenesis for more efficient xylose metabolization, allowing us to use lignocellulosic waste as feedstock. Also, we designed an automated closed-loop cell-computer interface to enhance production through dynamic regulation and stress regulation. The system obtains feedback from an optical density sensor and a stress-sensing fluorescent reporter. As we control gene expression with a blue light repressible promoter, blue LEDs activate and deactivate accordingly to maintain an optimal rate of production, eliminating the need for chemical inducers. We envision that our system has great potential for finer control and optimization as more functions are incorporated. | ||
Revision as of 21:16, 12 September 2018
Coup Dy'état: Eco-friendly Bio-manufacturing of Flavonoid Dyes in Escherichia coli via Computer-mediated Optogenetic Regulation
Synthetic clothing dyes are a major source of water pollution. Natural dyes are more beneficial to the environment, but production is land-intensive and low-yield. To increase the viability of natural dyes as an alternative to synthetic dyes, we developed a novel, eco-friendly method for the biomanufacturing of a natural flavonoid dye, luteolin, in E. coli. We performed mutagenesis for more efficient xylose metabolization, allowing us to use lignocellulosic waste as feedstock. Also, we designed an automated closed-loop cell-computer interface to enhance production through dynamic regulation and stress regulation. The system obtains feedback from an optical density sensor and a stress-sensing fluorescent reporter. As we control gene expression with a blue light repressible promoter, blue LEDs activate and deactivate accordingly to maintain an optimal rate of production, eliminating the need for chemical inducers. We envision that our system has great potential for finer control and optimization as more functions are incorporated.
HEAR OUR STORY
We’re using synthetic biology to change the way the world thinks and functions.
We’re a little disruptive, dangerous and maybe a tad too bold. But the world needs some shaking up every now and then, and we think we’re just the right people for that.
Together we’re going to engineer biology and create something awesome.
CONTACT US
nusigem@gmail.com21 Lower Kent Ridge Rd, Singapore 119077