Difference between revisions of "Template:Virginia/index"

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<h1 id="welcome-to-our-glorious-landing-page-">Welcome to our glorious Landing Page!</h1>
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* This page and wiki was built with the help of igem-wikibrick, a tool created by Virginia iGEM 2018
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<p>Heterogeneity of cell populations caused by <span class="tooltip">quorum sensing<span class="shortdef">The ability to detect and to respond to cell population density by gene regulation.</span><span class="longdef" tabindex="1">undefined</span> </span> leads to variability in gene expression that is hard to predict. During biomanufacturing, elevating quorum-induced protein expression will lead to gain of profit. Decreasing this expression can also be beneficial in situations where undesirable <span class="tooltip">biofilms<span class="shortdef">A protective, adhesive matrix of polymers typically produced after quorum activation.</span><span class="longdef" tabindex="1">undefined</span> </span> may form on medical equipment or controlling <span class="tooltip">virulence<span class="shortdef">The likelihood of a microbe to cause disease based on its phenotypic state.</span><span class="longdef" tabindex="1">undefined</span> </span> in bacteria. We will modify the existing bacterial quorum sensing system controlled by the <span class="tooltip">Lsr operon<span class="shortdef">LuxS Regulated (Lsr) operon responsive to AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span> by upregulating the synthesis and excretion of <span class="tooltip">Autoinducer-2<span class="shortdef">A universal signaling molecule used by microorganisms to coordinate group behavior through quorum sensing.</span><span class="longdef" tabindex="1">undefined</span> </span>, a universal quorum molecule. This will increase population-scale AI-2 intake and <span class="tooltip">phosphorylation<span class="shortdef"> The addition of a phosphate group to an organic compound. </span><span class="longdef" tabindex="1">undefined</span> </span> after the initial AI-2 threshold concentrations have been reached to reduce variability in induced gene expression.</p>
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* @link https://github.com/Virginia-iGEM/igem-wikibrick
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<p>A model is used to predict the impacts of manipulating the expression of quorum sensing genes and guide design and experimentation. DNA assembly of a Biobricks containing <span class="tooltip">pLsr<span class="shortdef">The bidirectional promoter of the Lsr Operon.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">T7 RNA Polymerase<span class="shortdef">An RNA polymerase from the T7 bacteriophage is highly selective for the pT7 promoter.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">LsrK<span class="shortdef">AI-2 kinase, which catalyzes the phosphorylation of A1-2 to phospho-AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">LsrACDB<span class="shortdef">Active import protein for AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">LuxS<span class="shortdef">An enzyme closely linked to the production of AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, <span class="tooltip">YdgG<span class="shortdef">Active export protein for AI-2.</span><span class="longdef" tabindex="1">undefined</span> </span>, and superfolding Green Fluorescent Protein (<span class="tooltip">sfGFP<span class="shortdef">superfolding Green Fluorescent Protein</span><span class="longdef" tabindex="1">undefined</span> </span>) enables both the enhancement of natural quorum sensing and the quantification of protein activation among the bacteria in a colony. This device will improve the viability of <span class="tooltip">autoinduction<span class="shortdef">The activation of a phenotype without external stimuli.</span><span class="longdef" tabindex="1">undefined</span> </span> as an induction method in industries such as biomanufacturing by decreasing the variability of a cell phenotypes and increasing expression within a single culture to reduce costs leading to an increase in profits. In addition, by insertion of T7 polymerase into a quorum-sensitive region of the genome of E. coli, we will create a chassis with specific, customizable quorum response for engineering and scientific applications.</p>
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<article class="banner">
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  <h3>Microbial Symphony</h3>
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    <h3>The Problem</h3>
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Revision as of 02:40, 14 September 2018

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Microbial Symphony

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 <section>

The Problem

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Scroll Down

To

Learn More

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The Solution

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