Difference between revisions of "Team:Mingdao/Description"
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<img class = "center" src="https://static.igem.org/mediawiki/2017/1/16/T--CSMU_NCHU_Taiwan--design-antidote.png" alt="" style="width:30%" id="antidote-banner"> | <img class = "center" src="https://static.igem.org/mediawiki/2017/1/16/T--CSMU_NCHU_Taiwan--design-antidote.png" alt="" style="width:30%" id="antidote-banner"> | ||
<h2>Thioredoxin fusion system</h2> | <h2>Thioredoxin fusion system</h2> | ||
| − | <p style="text-indent:2em" To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).> | + | <p style="text-indent:2em"> To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).</p> |
<img src="https://static.igem.org/mediawiki/2018/d/d5/T--Mingdao--project_picture_1.jpg" alt=""style="width:90%"> | <img src="https://static.igem.org/mediawiki/2018/d/d5/T--Mingdao--project_picture_1.jpg" alt=""style="width:90%"> | ||
</div> | </div> | ||
Revision as of 10:00, 15 September 2018
- EXPERIMENT
- Experiment
- MODELING
- Modeling
Design
Thioredoxin fusion system
To create a reporter system, we constructed a GFP expression vector. We amplified a constitutive promoter from Drosophila actin 5c gene and an eukaryotic poly A signal by PCR from pAc5.1 vector. The resulting DNA fragments were assembled with a BioBrick existing part of GFP to generate the reporter vector of Ac5-GFP-polyA / pSB1C3 (K2543004).
Reference
Antidote
Test Strip
Kit