Line 25: | Line 25: | ||
margin-top: 1em; | margin-top: 1em; | ||
margin-bottom: 1em; | margin-bottom: 1em; | ||
− | margin-left: | + | margin-left: 3em; |
− | margin-right: | + | margin-right: 3em; |
text-align: left; | text-align: left; | ||
− | font-size: | + | font-size: 20px; |
} | } | ||
</style> | </style> |
Revision as of 05:38, 17 September 2018
Description
Introduction
The Impacts of Excess Fluoride:
![](https://static.igem.org/mediawiki/2017/8/8a/T--East_Chapel_Hill--design-f1.png)
Source: http://www.bgs.ac.uk/research/groundwater/health/fluoride.html
Solution
What is a Riboswitch?
![](https://static.igem.org/mediawiki/2017/e/ea/T--East_Chapel_Hill--project.png)
2015 Exeter iGEM Team, RNA Riboswitches
![](https://static.igem.org/mediawiki/2017/e/e5/ T--East_Chapel_Hill--design-f3.png)
Aiming Ren, Kanagalaghatta R. Rajashankar, Dinshaw J. Patel “Fluoride ion encapsulation by Mg2+ ions and phosphates in a fluoride riboswitch” 2012 Nature 486, 85–89
![](https://static.igem.org/mediawiki/2017/5/50/ T--East_Chapel_Hill--design-f4.png)
Randy B. Stockbridge, Ludmila Kolmakova-Partensky, Tania Shane, Akiko Koide, Shohei Koide, Christopher Miller & Simon Newstead "Crystal structures of a double-barrelled fluoride ion channel." 2015 Nature 525, 548-51
Our Design
![](https://static.igem.org/mediawiki/2017/e/ef/T--East_Chapel_Hill--design-f5.png)
How CHOP works:
- Using the ΔcrcB E. coli strain, which can accumulate fluoride intracellularly
- The Riboswitch detects fluoride
- Fluoride activates the chloramphenicol acetyltransferase enzyme
- Which allows for the growth of bacteria on agar plates with the antibiotic chloramphenicol
References
- Using the ΔcrcB E. coli strain, which can accumulate fluoride intracellularly
- The Riboswitch detects fluoride
- Fluoride activates the chloramphenicol acetyltransferase enzyme
- Which allows for the growth of bacteria on agar plates with the antibiotic chloramphenicol