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− | + | The outbreak of green algae is a serious natural disaster, which also threatens social economy and the health of human beings. The periodically occurrence of <i>Enteromorpha</i> along the coastline has been a stubborn local environmental problem here in Qingdao, Shandong, China. | |
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− | + | At the same time, we respond to the third generation of biofuel production, in which algae, as an ideal source, takes a dominant position. Compared with traditional terrestrial plant used for biofuel like corn, straw and sugarcane, algae has much less lignin and more softer cellulose, which makes it easier to transform into fuel like ethanol.[1] In addition, algae as a marine plant, does not require land source at all. This can be a great advantage in the current world where population makes a big problem and the land is much more precious than ever. | |
− | + | Considering these two reasons, our initial project was born. We decided to make use of <i>Enteromorpha</i> residue and turn it into ethanol in a synthetic biology way. | |
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− | + | <img src="https://static.igem.org/mediawiki/2017/e/e5/T--OUC-China--des1.jpg" width="350"><br/> | |
+ | Figure 1.1 <i>Enteromorpha</i> outbreak in Qingdao<br/> | ||
+ | Figure 1.2 Traditional materials for cellulose fermentation<br/> | ||
+ | Figure 1.3 Cellulose in plant<br/> | ||
+ | Figure 1.4 Cellulose in algae | ||
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Revision as of 00:39, 21 September 2018
MEMBER'S ATTRIBUTION
From the beginning of 2018, OUC-China have brand new members for this competition season. With the help of experienced team leaders before, we build a team consists of 8 undergraduate students from different majors and two grades. And by sharing happiness and woe this season, now we become close friends and have the tacit understanding. All the members in 2018 OUC-China devoted the best of themselves to develop the project. At the same time, we would have gone nowhere without the help from many other people from our school, other institutions and the society. We sincerely appreciate all the help!
Yunqian Zhang, captain of OUC-China iGEM 2018 and general affair supervisor, was responsible for designing our project and experiment. She designed and checked all the circuit as well as the primers used in PCR and part standardization. She provided basic experimental scheme for lab administrator. She was responsible for construct and test by microplate reader of polycistron circuit. Also she arranged journals for all people to CCiC and Boston.
Yanju Chen, vice-captain of our team, pushed the preceding of human practice and communication with other teams. She keep connection with all the professors who helped us. She is also responsible for submitting parts and document the experimental characterization. Also she arranged journals for all people to Beijing.
Jiacheng Huang, vice-captain of our team. He selected all the csy4 mutants and hairpin mutants to meet our goal. He was in charge of modeling and excavated deeply in theory who also is one of wiki designer.
Qinbo Qiao, lab administrator and arranged the journal to get visa. Her major work was part standardization and extracted a mount of DNA.
Xudong Liu, lab administrator, was in charge of construct circuits by using 3A assembly and detected the expression of proteins. Also he was responsible for the measurement of the growth rate of E.coli, providing experimental limits for model. He changed our experimental scheme with need at any moment.
Qingjie Li, the financial manager of our team and designer for our Video Game——Csy4 Go! She was also responsible for our motA test system. She designed the experiment for this system and finished all the lab work for this part by herself. She helped us with a lot of biological order for biotechnology companies.
Kai Sun, the web designer and art designer who established the structure of our wiki, poster, team uniform and many other designing works. At the same time, he also take the task of Interlab measurement and primers design.
Anyi Li, was responsible for primers design and plan for human practice. At the same time, she test the second system which contain many Csy4 mutants and hairpin mutants by flow cytometry for 6 times. And she writed our notebook and took part in some lab work.
Acknowledgement for support
Every member in 2017 OUC-China iGEM gave us practical suggestions.
Yang Liu, the Captain of OUC-China 2011 and Li Kang, the Captain of OUC-China 2012 helped improve the synthetic conception of our project.
Our PI Prof. Chenguang Liu and Prof. Xingguo Liang offered us constructive advice over our project and gave us long-term technical consulting support through the procedure.
Prof Chunbo Lou and Pei Du, from Institute of Microbiology, provided us with original idea by their work and also taught us with some details about experiment and lab work.
Our PI Prof.Xingguo Liang offered us experimental lab.
Our PI Prof.Zhe Chi helped us with gene synthesis and financial problems, at the same time, he also provided us with unique advice about project.
Prof. Xiaohua Zhang, from College of Marine Life, Ocean University of China, provided us instrument (Thermo Scientific NanoDrop) to measure the concentration of nucleic acid.
Prof Mingzhuang Zhu and Wei Yang provided us the flow cytometry which was really helpful for our second system.
The outbreak of green algae is a serious natural disaster, which also threatens social economy and the health of human beings. The periodically occurrence of Enteromorpha along the coastline has been a stubborn local environmental problem here in Qingdao, Shandong, China.
At the same time, we respond to the third generation of biofuel production, in which algae, as an ideal source, takes a dominant position. Compared with traditional terrestrial plant used for biofuel like corn, straw and sugarcane, algae has much less lignin and more softer cellulose, which makes it easier to transform into fuel like ethanol.[1] In addition, algae as a marine plant, does not require land source at all. This can be a great advantage in the current world where population makes a big problem and the land is much more precious than ever. Considering these two reasons, our initial project was born. We decided to make use of Enteromorpha residue and turn it into ethanol in a synthetic biology way.
Figure 1.1 Enteromorpha outbreak in Qingdao
Figure 1.2 Traditional materials for cellulose fermentation
Figure 1.3 Cellulose in plant
Figure 1.4 Cellulose in algae