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Our main system also called “Nutrient System”  deals with the dependence of the three organisms on the availability of the essential nutrients.
 
 
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Revision as of 15:29, 23 September 2018

Attributions

Behind every good team is an even better team - same with us this year. Without you our project would not have been realized this way. Thank you for having our back and showing us what is important when building a science project.

Team

Name Responsible Involved
Carina Laboratory: growth curves, biosensors
Outreach: Postcard Project, Networking, Social media (Instagram), presentation, Cyano2018 conference, proofreading, wiki
Outreach: radio interviews Laboratory: co-culture experiments, Cyano, Lab inventory; collaborations
Jan - -
Jennifer - -
Katharina Laboratory: Yeast cloning in quorum sensing, new approach for the sythetic promotor
Outreach: Outreach manager (supervising everything), Wiki, poster and presentation designer, judging form
Laboratory: E. coli cloning in quorum sensing, part submission, grwoth curves
Outreach: Financial stuff, organizing Boston, skype meetings
Miriam Laboratory: working with Cyanobacteria, microscopy
OutreachIntegrated Human Practice: Pamela Silver, Danny Ducat
Outreach: SynBio lecture series
Nicholas - -
Salima Laboratory: Cloning of S. cerevisiae for Auxotrophy-Trinity
OutreachSocial media, Safety Form, room maiden, Good-Noodles-Star warder
Outreach: SynBio lecture series, postcard project, panel discussion, integrated human practice, collaborations, networking, presentations
Laboratory: Growth curves, Co-culture experiments
Sarah Laboratory: Cloning of S. cerevisiae for Auxotrophy-Trinity
OutreachSocial media, Safety Form, room maiden, Good-Noodles-Star warder
Outreach: SynBio lecture series, postcard project, panel discussion, integrated human practice, collaborations, networking, presentations
Laboratory: Growth curves, Co-culture experiments
Susanne Laboratory: nutrient system E. coli, S. cerevisiae
Outreach: project description
Outreach: Events, vBio presentation
Svenja Outreach: activities, Wiki, postcard project
Laboratory: Synthetic promoter new approach
Laboratory: Growth curves, co-culture experiments
Thomas - -
Ylenia LaboratoryLab manager, lab Safety, Quorum sensing manager, Interlab challenge, part submissions, judging form, lab advices Outreach: Radio interviews, Oorganisational stuff, collaborations, skype meetings, wiki textes, growth cultures, aponsoring

Advisor

Name Responsible Involved
Anna - -
Kai Supervising auxtotrophies, brainstorming advice, team building, travel organisation, S. cerevisiae expert, scientific proofreading Social Media (Twitter), Quorum sensing brainstorming, check protocols/virtual cloning, poster and presentation training
Maximilian - -
Nicolas - -
Philipp - -
Tim - -

Introduction

E.coli:
A synthetic cluster of 6 genes from different organisms (Acidovorax avenae, E. coli, Pseudomonas sp., Rhodococcus sp., S. cerevisiae) used for the cleavage of melamin to ammonia and carbon dioxide. The ammonia diffuses out of the cell.

S. cerevisiae:
With the gene ptxD from P. putida, S. cerevisiae converts the unusable phosphite into phosphate. The phosphate exporter XPR1 from H. sapiens may help to increase the extracellular phosphate concentration.

S. elongatus
Converting sucrose, which is naturally produced by photosynthesis, into fructose and glucose by an invertase encoded by the gene invA. Glucose will be secreted into the media with the help of an exporter, encoded by glf, and thus providing enough glucose for the whole Trinity. The genes are taken from the Z. mobilis genome.