Difference between revisions of "Team:Tacoma RAINmakers/Experiments"

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<h3>What should this page contain?</h3>
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<h3>PCR Amplification
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</h3>
 
<ul>
 
<ul>
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<li> Protocols </li>
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<li> In order to begin the cloning process, individual portions (amilCP, spisPink, PcArsR) of our insert must be amplified to facilitate digestion and ligation of our plasmid. This PCR involves two different primer pairs; one pair matches to the chromoproteins and the other matches to the arsenic regulator.
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</li>
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<li>
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Component
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Reaction Volume (µL)
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GoTaq Green Buffer
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5
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Forward Primer A/B (10µM)
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2.5
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Reverse Primer C/D (10µM)
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2.5
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Magnesium Chloride
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2
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Insert DNA (20ng/µL)
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0.25 - 0.66
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dNTP
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0.5
 +
Polymerase
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0.25
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Molecular Water
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to 20
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</li>
 
<li> Experiments </li>
 
<li> Experiments </li>
 
<li> Documentation of the development of your project </li>
 
<li> Documentation of the development of your project </li>

Revision as of 21:13, 23 September 2018

Team:TacomaRAINmakers/Notebook - 2017.igem.org

Team:ECUST/Lab/Notebook

Experiments

Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.

Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.

PCR Amplification

  • In order to begin the cloning process, individual portions (amilCP, spisPink, PcArsR) of our insert must be amplified to facilitate digestion and ligation of our plasmid. This PCR involves two different primer pairs; one pair matches to the chromoproteins and the other matches to the arsenic regulator.
  • Component Reaction Volume (µL) GoTaq Green Buffer 5 Forward Primer A/B (10µM) 2.5 Reverse Primer C/D (10µM) 2.5 Magnesium Chloride 2 Insert DNA (20ng/µL) 0.25 - 0.66 dNTP 0.5 Polymerase 0.25 Molecular Water to 20
  • Experiments
  • Documentation of the development of your project

Inspiration