Difference between revisions of "Team:SCAU-China/Interlab"

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         <p>comming soon...</p>
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         <p>The goal of the iGEM InterLab Study is reliable and repeatable measurement which is a key component to synthetic biology. We took part in the 5th International InterLab Measurement Study and the big question is</p>
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       <p></p>
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       <p> “Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD? ”</p>
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       <p></p>
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      <h1>Parts</h1>
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       <p>Interlab test devices used, as set by the iGEM measurement committee</p>
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      <table>
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      <th>
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      <td>Device</td>
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      <td>Part Number</td>
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      <td>Location</td>
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      </th>
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      <tr>
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      <td>Negative control</td>
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      <td>BBa_R0040</td>
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      <td>Well 2D</td>
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      </tr>
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    <tr>
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      <td>Positive control</td>
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      <td>BBa_I20270</td>
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      <td>Well 2B</td>
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      </tr>
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      <tr>
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      <td>Test Device 1</td>
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      <td>BBa_J364000</td>
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      <td>Well 2F</td>
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      </tr>
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      <tr>
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      <td>Test Device 2</td>
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      <td>BBa_J364001</td>
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      <td>Well 2H</td>
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      </tr>
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      <tr>
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      <td>Test Device 3</td>
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      <td>BBa_J364002</td>
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      <td>Well 2J</td>
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      </tr>
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      <tr>
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      <td>Test Device 4</td>
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      <td>BBa_J364007</td>
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      <td>Well 2L</td>
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      </tr>
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      <tr>
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      <td>Test Device 5</td>
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      <td>BBa_J364008</td>
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      <td>Well 2N</td>
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      </tr>
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      <tr>
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      <td>Test Device 6</td>
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      <td>BBa_J364009</td>
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      <td>Well 2P</td>
 +
      </tr>
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      </table>
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      <p>the preparation of competent cells(Escherichia coli strain DH5α) and their transformation according to the cell measurement protocol. Meanwile, we followed the Calibration Protocols supplied by the iGEM HQ to generate OD600 Reference point, Particle Standard Curve and Fluorescence standard curve to calibrate the plate reader(96 well plate) and then measured both the fluorescence and absorbance of our samples in 0-hour and 6-hour.  </p>
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      <p>We used the set protocol that we were given so as to ensure everyone was doing the same thing. These can be found at <a href="https://2018.igem.org/Team:SCAU-China/Protocol">this</a></p>
  
 
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Revision as of 14:50, 27 September 2018

SCAU-2018
interlab

The goal of the iGEM InterLab Study is reliable and repeatable measurement which is a key component to synthetic biology. We took part in the 5th International InterLab Measurement Study and the big question is

“Can we reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD? ”

Parts

Interlab test devices used, as set by the iGEM measurement committee

Device Part Number Location
Negative control BBa_R0040 Well 2D
Positive control BBa_I20270 Well 2B
Test Device 1 BBa_J364000 Well 2F
Test Device 2 BBa_J364001 Well 2H
Test Device 3 BBa_J364002 Well 2J
Test Device 4 BBa_J364007 Well 2L
Test Device 5 BBa_J364008 Well 2N
Test Device 6 BBa_J364009 Well 2P

the preparation of competent cells(Escherichia coli strain DH5α) and their transformation according to the cell measurement protocol. Meanwile, we followed the Calibration Protocols supplied by the iGEM HQ to generate OD600 Reference point, Particle Standard Curve and Fluorescence standard curve to calibrate the plate reader(96 well plate) and then measured both the fluorescence and absorbance of our samples in 0-hour and 6-hour.

We used the set protocol that we were given so as to ensure everyone was doing the same thing. These can be found at this

School's name:SCAU

Member's name:SCAU

Designed by:SCAU