Difference between revisions of "Team:Munich/recbiobricks.html"

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       <td>Notes:</td>
 
       <td>Notes:</td>
 
       <td>Primers: VF2 & VR
 
       <td>Primers: VF2 & VR
 +
</td>
 +
    </tr>
 +
<tr>
 +
      <td>Results:</td>
 +
      <td>?</td>
 +
    </tr>
 +
</table>
 +
 +
<h3>9/3</h3>
 +
<h4>Removing PstI restriction site from pSB1C3_RecB</h4>
 +
<table class="table table-borderless">
 +
    <tr>
 +
 +
      <td>Participants:</td>
 +
      <td>eni</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Protocol:</td>
 +
      <td>PCR, PCR purification, Ligation, gibson assembly, trafo</td>
 +
    </tr>
 +
    <tr>
 +
      <td>Notes:</td>
 +
      <td>because of time restraints, this experiment was done using ligation and gibson assembly approaches in parallel <br>
 +
PCR: RecB_BB_PstI-remove-Lig
 +
Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv
 +
Templ: RecB_BioBrick Plasmid
 +
AT: 72° ET: 11 min
 +
<br>
 +
PCR: RecBCD_BB_PstI_remove_lig
 +
Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv
 +
Templ: RecBCD_BioBrick Plasmid
 +
AT: 72° ET: 11 min
 +
<br>
 +
PCR: RecBCD-TEV_BB_PstI_remove-Lig
 +
Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv
 +
Templ: RecBCD-TEV_BioBrick Plasmid
 +
AT: 72° ET: 11 min
 +
<br>
 +
PCR: RecB_BB_PstI-remove-GA
 +
Primer: RecB_mut_fw & RecB_mut_rv
 +
Templ: RecB_BioBrick Plasmid
 +
AT: 72° ET: 11 min
 +
<br>
 +
PCR: RecBCD_BB_PstI_remove_GA
 +
Primer: RecB_mut_fw & RecB_mut_rv
 +
Templ: RecBCD_BioBrick Plasmid
 +
AT: 72° ET: 11 min
 +
<br>
 +
PCR: RecBCD-TEV_BB_PstI_remove-GA
 +
Primer: RecB_mut_fw & RecB_mut_rv
 +
Templ: RecBCD-TEV_BioBrick Plasmid
 +
AT: 72° ET: 11 min
 
</td>
 
</td>
 
     </tr>
 
     </tr>

Revision as of 15:53, 28 September 2018


8/31

Assembling biobrick for RecB, C, D

Participants: eni, katja
Protocol: PCR, agarose gel, gel extraction, ligation, chem trafo, miniprep
Notes: RecD_BB_EcoRI_fw & RecD_BB_SpeI_rv (3,5 kb)

RecB_BB_EcoRI_fw & RecB_BB_EcoRi_rv (1,8 kb)
RecC_BB_EcoRI_fw & RecB_BB_EcoRI_rv (3,3 kb)
AT 68° ET 70 s

Results: PIC only one worked, but somehow all were ligated :D

9/3

Test pcr for Biobricks of RecB, C, D

Participants: eni
Protocol: PCR, agarose gel
Notes: Primers: VF2 & VR
Results: ?

9/3

Removing PstI restriction site from pSB1C3_RecB

Participants: eni
Protocol: PCR, PCR purification, Ligation, gibson assembly, trafo
Notes: because of time restraints, this experiment was done using ligation and gibson assembly approaches in parallel

PCR: RecB_BB_PstI-remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD_BB_PstI_remove_lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD-TEV_BB_PstI_remove-Lig Primer: RecB_mut_Lig_fw & RecB_mut_Lig_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecB_BB_PstI-remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecB_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD_BB_PstI_remove_GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD_BioBrick Plasmid AT: 72° ET: 11 min
PCR: RecBCD-TEV_BB_PstI_remove-GA Primer: RecB_mut_fw & RecB_mut_rv Templ: RecBCD-TEV_BioBrick Plasmid AT: 72° ET: 11 min

Results: ?