Difference between revisions of "Team:Munich/chibiobrick1.html"

Revision as of 10:08, 29 September 2018

Participants:	Dominic
Protocols:	restriction digest, Ligation, chem trafo, mini-prep, sequencing
Notes:	digest with XbaI, SpeI transformation of Dh5a
Results:	no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment.

Participants:	Dominic
Protocol:	restriction digest, PCR purification, ligation, chem trafo, PCR, Agarose gel
Notes:	digest with XbaI, SpeI transformation of Dh5a
Results:	No bands on the Gel. Colonies on the plate resulted from contamination.

@@ Line 2: / Line 2: @@
 <div class="event-info">
-<h3>6/4</h3>
+<h3>2018/06/04</h3>
 <h4>Assembling pSB1C3_Chi6 with A3 Assembly</h4>
 <table class="table table-borderless">
@@ Line 26: / Line 26: @@
 </table>
-<h3>6/6 - 6/26</h3>
+<h3>2018/06/06 - 2018/06/26</h3>
 <h4>Redo: Assembling pSB1C3_Chi6 with A3 Assembly</h4>
 <table class="table table-borderless">
@@ Line 41: / Line 41: @@
        <td>Notes:</td>
        <td> digest with XbaI, SpeI <br>
-Additionally, samples were purified after the restriction digest <br>
+transformation of Dh5a
-transformation of Dh5a <br>
-test pcr was performed with VF2 and VR primers
 </td>
      </tr>
   <tr>
        <td>Results:</td>
-       <td>No bands on the Gel. Colonies on the plate resulted from contamination. We decided to postpone the experiment because everyone in the lab was having difficulities with cloning.</td>
+       <td>No bands on the Gel. Colonies on the plate resulted from contamination.</td>
      </tr>
 </table>