Difference between revisions of "Team:Munich/chibiobrick1.html"
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<h4>Forming oligodimers from single-strand DNA chi6 sequences</h4> | <h4>Forming oligodimers from single-strand DNA chi6 sequences</h4> | ||
| + | <em>2018/06/01</em> | ||
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<h4>Assembling pSB1C3_Chi6 with A3 Assembly</h4> | <h4>Assembling pSB1C3_Chi6 with A3 Assembly</h4> | ||
| + | <em>2018/06/04</em> | ||
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<h4>Redo: Assembling pSB1C3_Chi6 with A3 Assembly</h4> | <h4>Redo: Assembling pSB1C3_Chi6 with A3 Assembly</h4> | ||
| + | <em>2018/06/06 - 2018/06/26</em> | ||
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Revision as of 10:36, 29 September 2018
Forming oligodimers from single-strand DNA chi6 sequences
2018/06/01| Participants: | Dominic |
| Protocol: | Oligodimerization |
Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/04| Participants: | Dominic |
| Protocols: | restriction digest, Ligation, chem trafo, mini-prep, sequencing |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | no sequence obtained for the given primers. The samples must be contaminated. We decided to redo the experiment. |
Redo: Assembling pSB1C3_Chi6 with A3 Assembly
2018/06/06 - 2018/06/26| Participants: | Dominic |
| Protocol: | restriction digest, PCR purification, ligation, chem trafo, PCR, Agarose gel |
| Notes: | digest with XbaI, SpeI transformation of Dh5a |
| Results: | No bands on the Gel. Colonies on the plate resulted from contamination. |