Difference between revisions of "Team:Munich/preparingge.html"
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<h4>Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering</h4> | <h4>Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering</h4> | ||
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| − | <td>inoculate pRED at 30°C because of temperature sensitive promoter</td> | + | <td>inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid.</td> |
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Revision as of 14:23, 29 September 2018
Transforming E. Coli DH5a to amplify plasmids for pRED/ET engineering
2018/08/28
| Participants: | Dominic |
| Protocol: | epo. Trafo |
| Notes: | inoculate pRED/ET at 30°C because of temperature sensitive promoter on the plasmid. |
| Results: | ~200 colonies |