Difference between revisions of "Team:Edinburgh OG"

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                                <span>Antibiotic revolution: a molecular toolkit to re-sensitise </span><br>
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                                <span class="cd-words-wrapper">
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                                    <b class="is-visible">Methycillin resistant S.aureus (MRSA)</b>
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                                    <b>Vancomycin resistant S.aureus (VRSA)</b>
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                                    <b>Klebsiella pneumoniae</b>
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                                    <b>Pseudomonas aeruginosa</b>
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                                    <b>Enterococcus faecalis/faecium</b>
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                                    <b>Acinetobacter baumannii</b>
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                                </h1>
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<h2 class="wow fadeInUp animated" data-wow-delay=".6s">
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                                    Modular molecular toolkit for re-sensitisation of antibiotic-resistant pathogens using CRISPR delivered by a two-phage system.
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                                </h2>
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<a class="btn-lines dark light wow fadeInUp animated smooth-scroll btn btn-default btn-green" data-wow-delay=".9s" href="#works" data-section="#about">Read more</a>
  
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<h2>
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                                ABOUT PhagED
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                                </h2>
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<p>
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The threat posed by antibiotic resistant bacteria is a pressing issue which must be addressed. It is difficult and expensive to develop new antibiotics, so our project is designed to make currently available antibiotics useful again. Our aim is to create
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a toolkit to re-sensitise pathogens to antibiotics using CRISPR and a two-phage system, based on work by <b style="font-weight: bold;">Yosef et al. (2015, doi:10.1073/pnas.1500107112)</b>. An engineered lysogenic phage will transfer a CRISPR
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system to its host bacterium, designed to cleave resistance genes and also confer protection from an engineered lytic phage. When this lytic phage is added to the population, it kills any bacteria that have not been successfully re-sensitised. We
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chose to target genes found in the highly-resistant ESKAPE pathogens, and worked with 4 different phages - P1, lambda, T4 and T7. Our system was modelled in silico and tested empirically on a specially designed E. coli testing platform.
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</p>
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<p>
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Figure: Yosef et al., 2015
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<img src="https://static.igem.org/mediawiki/2017/a/ae/T--Edinburgh_OG--about.png" alt="">
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<div class="col-sm-8 col-xs-12 col-sm-offset-2">
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<div class="section-heading">
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<h1 class="title wow fadeInDown" data-wow-delay=".3s">TAKE A CLOSER LOOK</h1>
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<p class="wow fadeInDown" data-wow-delay=".5s" style="display:none;">
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Aliquam lobortis. Maecenas vestibulum mollis diam. Pellentesque auctor neque nec urna. Nulla sit amet est. Aenean posuere <br> tortor sed cursus feugiat, nunc augue blandit nunc, eu sollicitudin urna dolor sagittis lacus.
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</p>
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<figure class="wow fadeInLeft animated portfolio-item" data-wow-duration="500ms" data-wow-delay="0ms">
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<div class="img-wrapper">
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<img src="https://static.igem.org/mediawiki/2017/e/ea/T--Edinburgh_OG--item-1.png" class="img-responsive" alt="this is a title">
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<div class="overlay">
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Description">Details</a>
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</div>
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<figcaption>
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<h4>
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                                <a href="https://2017.igem.org/Team:Edinburgh_OG/Description">
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                                    Project
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                                </a>
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                                </h4>
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<p>
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Get to know PhagED better.
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</p>
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<div class="col-sm-6 col-xs-12">
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<img src="https://static.igem.org/mediawiki/2017/9/96/T--Edinburgh_OG--item-2.png" class="img-responsive" alt="this is a title">
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<div class="overlay">
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<div class="buttons">
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Parts">Details</a>
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</div>
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<figcaption>
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<h4>
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                                <a href="https://2017.igem.org/Team:Edinburgh_OG/Parts">
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                                    Parts
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                                </a>
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                                </h4>
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<p>
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Blocks of DNA that we've used.
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</p>
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<div class="overlay">
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<div class="buttons">
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/Safety">Details</a>
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</div>
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</div>
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</div>
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<figcaption>
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<h4>
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                                <a href="https://2017.igem.org/Team:Edinburgh_OG/Safety">
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                                    Safety
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                                </a>
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                                </h4>
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<p>
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Safety always comes first.
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</p>
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</figcaption>
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</figure>
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<div class="col-sm-6 col-xs-12">
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<figure class="wow fadeInLeft animated" data-wow-duration="500ms" data-wow-delay="600ms">
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<div class="img-wrapper">
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<img src="https://static.igem.org/mediawiki/2017/8/87/T--Edinburgh_OG--item-4.png" class="img-responsive" alt="">
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<div class="overlay">
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<div class="buttons">
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<a target="_blank" href="https://2017.igem.org/Team:Edinburgh_OG/HP/Silver">Details</a>
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</div>
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</div>
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</div>
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<figcaption>
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<h4>
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                                <a href="https://2017.igem.org/Team:Edinburgh_OG/HP/Silver">
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                                    Human Practices
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                                </a>
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                                </h4>
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<p>
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How does PhagED fit into the context of our lives?
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</p>
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</figcaption>
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</figure>
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</div>
  
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<h1> Edinburgh OG </h1>
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</div>
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<h2> About </h2>
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</div>
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<p> The University of Edinburgh Overgraduate iGEM team this year is looking at making bioplastics. We want to look at a more innovative source for useful biodegradable plastics. Our plastic of choice is called poly(hydroxybutyrate-co-hydroxyvalerate) or PHBV. We are looking at using by-product from whisky distilleries, namely pot ale, to produce this plastic. PHBV holds a lot of promise because of its physical properties and can fit into the current plastic market and by using bio-mass, waste, or secondary carbon sources we are looking to decouple plastic production from petroleum. PHBV can also be biodegraded unlike most currently used plastics reducing the amount of devastating waste thrown into the environment. To create this plastic, we are trying to introduce enzymes into Escherichia coli to facilitate this production of PHBV efficiently.   </p>
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<h1 class="title wow fadeInDown" data-wow-delay=".3s">Offer From Me</h1>
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<p class="wow fadeInDown" data-wow-delay=".5s">
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Lorem ipsum dolor sit amet, consectetur adipisicing elit. Sed,<br> quasi dolores numquam dolor vero ex, tempora commodi repellendus quod laborum.
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</p>
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<h4 class="media-heading">Media heading</h4>
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</div>
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<div class="media-body">
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<h4 class="media-heading">Well documented.</h4>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</div>
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</div>
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<div class="media wow fadeInDown animated" data-wow-duration="500ms" data-wow-delay="900ms">
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<i class="ion-ios-lightbulb-outline"></i>
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</div>
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</div>
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<div class="media-body">
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<h4 class="media-heading">Well documented.</h4>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</div>
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</div>
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</div>
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<i class="ion-ios-americanfootball-outline"></i>
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</div>
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<div class="media-body">
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<h4 class="media-heading">Free updates</h4>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</div>
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</div>
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<i class="ion-ios-keypad-outline"></i>
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</div>
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<div class="media-body">
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<h4 class="media-heading">Solid Support</h4>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</div>
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</div>
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<i class="ion-ios-barcode-outline"></i>
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</div>
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</div>
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<div class="media-body">
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<h4 class="media-heading">Simple Installation</h4>
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<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptatum, sint.</p>
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</div>
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</div>
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</div>
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</div>
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</div>
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</section>
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<!--
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      Team Section Start
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  ================================================== -->
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<section id="team">
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<div class="container">
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<div class="row">
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<h2 class="subtitle text-center">Meet The Team</h2>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/e/e6/T--Edinburgh_OG--filippo.png" class="team-pic" alt="">
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</div>
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<h3 class="team_name">Filippo Abbondanza</h3>
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<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
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<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
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<p class="social-icons">
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<a href="https://www.facebook.com/filippo.abbondanza" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
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<a href="https://www.linkedin.com/in/filippo-abbondanza-a9130897" target="_blank"><i class="ion-social-linkedin-outline"></i></a>
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</p>
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</div>
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</div>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".5s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/8/8e/T--Edinburgh_OG--erin.png" class="team-pic" alt="">
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</div>
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<h3 class="team_name">Erin Corbett</h3>
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<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
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<p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform, and engineering the lytic T7 phage.</p>
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<p class="social-icons">
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</p>
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</div>
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</div>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".7s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/a/a5/T--Edinburgh_OG--rikki.jpg" class="team-pic" alt="">
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</div>
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<h3 class="team_name">Yunqi He</h3>
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<p class="team_designation">MSc Biochemistry</p>
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<p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
 +
<p class="social-icons">
 +
<a href="https://www.facebook.com/y.q.heybd" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
  
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</p>
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</div>
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</div>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".9s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/3/35/T--Edinburgh_OG--ti.jpg" class="team-pic" alt="">
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<h3 class="team_name">Ti He</h3>
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<p class="team_designation">MSc Biotechnology</p>
 +
<p class="team_text">Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.</p>
 +
<p class="social-icons">
 +
<a href="https://www.facebook.com/profile.php?id=100012772305809" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
 +
</p>
 +
</div>
 +
</div>
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</div>
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<div class="row">
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".3s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/f/fa/T--Edinburgh_OG--lydia.jpg" class="team-pic" alt="">
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</div>
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<h3 class="team_name">Lydia Mapstone</h3>
 +
<p class="team_designation">MSc Synthetic Biology & Biotechnology</p>
 +
<p class="team_text">Engineering <i>E. coli</i> to create our mock pathogen testing platform and engineering the lytic T4 phage using BRED.</p>
 +
<p class="social-icons">
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</p>
 +
</div>
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</div>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".5s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/2/27/T--Edinburgh_OG--yuri.jpg" class="team-pic" alt="">
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</div>
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<h3 class="team_name">Yuri Matsueda</h3>
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<p class="team_designation">MSc Biotechnology</p>
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<p class="team_text">Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.</p>
 +
<p class="social-icons">
 +
<a href="https://www.facebook.com/profile.php?id=100004212933551" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
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</p>
 +
</div>
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</div>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".7s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/d/d8/T--Edinburgh_OG--anton.jpg" class="team-pic" alt="">
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</div>
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<h3 class="team_name">Anton Puzorjov</h3>
 +
<p class="team_designation">MSc Bioinformatics</p>
 +
<p class="team_text">Building a model of bacteria-phage interactions in two-step re-sensitisation combining both lysogenic and lytic phages.</p>
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<p class="social-icons">
 +
<a href="https://www.facebook.com/puzorjov" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
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<a href="https://twitter.com/AntonPuzorjov" target="_blank"><i class="ion-social-twitter-outline"></i></a>
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<a href="https://www.linkedin.com/in/antonpuzorjov/" target="_blank"><i class="ion-social-linkedin-outline"></i></a>
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</p>
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</div>
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</div>
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<div class="col-md-3">
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<div class="team-member wow fadeInLeft" data-wow-duration="500ms" data-wow-delay=".9s">
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<div class="team-img">
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<img src="https://static.igem.org/mediawiki/2017/7/70/T--Edinburgh_OG--yating.jpg" class="team-pic" alt="">
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</div>
 +
<h3 class="team_name">Yating Wang</h3>
 +
<p class="team_designation">MSc Drug Discovery & Translational Biology</p>
 +
<p class="team_text">Engineering the lysogenic phage P1 with a CRISPR system to target resistance gene fragments.</p>
 +
<p class="social-icons">
 +
<a href="https://www.facebook.com/profile.php?id=100010921308790" class="facebook" target="_blank"><i class="ion-social-facebook-outline"></i></a>
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</p>
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</div>
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</div>
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</div>
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<br>
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<div class="row">
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Revision as of 14:33, 29 September 2018

PhagED: a molecular toolkit to re-sensitise ESKAPE pathogens

Antibiotic revolution: a molecular toolkit to re-sensitise
Methycillin resistant S.aureus (MRSA) Vancomycin resistant S.aureus (VRSA) Klebsiella pneumoniae Pseudomonas aeruginosa Enterococcus faecalis/faecium Acinetobacter baumannii

Modular molecular toolkit for re-sensitisation of antibiotic-resistant pathogens using CRISPR delivered by a two-phage system.

Read more

ABOUT PhagED

The threat posed by antibiotic resistant bacteria is a pressing issue which must be addressed. It is difficult and expensive to develop new antibiotics, so our project is designed to make currently available antibiotics useful again. Our aim is to create a toolkit to re-sensitise pathogens to antibiotics using CRISPR and a two-phage system, based on work by Yosef et al. (2015, doi:10.1073/pnas.1500107112). An engineered lysogenic phage will transfer a CRISPR system to its host bacterium, designed to cleave resistance genes and also confer protection from an engineered lytic phage. When this lytic phage is added to the population, it kills any bacteria that have not been successfully re-sensitised. We chose to target genes found in the highly-resistant ESKAPE pathogens, and worked with 4 different phages - P1, lambda, T4 and T7. Our system was modelled in silico and tested empirically on a specially designed E. coli testing platform.

Figure: Yosef et al., 2015

TAKE A CLOSER LOOK

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Project

Get to know PhagED better.

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Parts

Blocks of DNA that we've used.

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Safety

Safety always comes first.

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Human Practices

How does PhagED fit into the context of our lives?

Meet The Team

Filippo Abbondanza

MSc Synthetic Biology & Biotechnology

Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.

Erin Corbett

MSc Synthetic Biology & Biotechnology

Engineering E. coli to create our mock pathogen testing platform, and engineering the lytic T7 phage.

Yunqi He

MSc Biochemistry

Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.

Ti He

MSc Biotechnology

Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.

Lydia Mapstone

MSc Synthetic Biology & Biotechnology

Engineering E. coli to create our mock pathogen testing platform and engineering the lytic T4 phage using BRED.

Yuri Matsueda

MSc Biotechnology

Engineering the lysogenic P1 phage with a CRISPR system to target resistance gene fragments.

Anton Puzorjov

MSc Bioinformatics

Building a model of bacteria-phage interactions in two-step re-sensitisation combining both lysogenic and lytic phages.

Yating Wang

MSc Drug Discovery & Translational Biology

Engineering the lysogenic phage P1 with a CRISPR system to target resistance gene fragments.


Owen Yeung

MSc Synthetic Biology & Biotechnology

Engineering the lysogenic lambda phage with a CRISPR system to target resistance gene fragments.

The Edinburgh_OG team members developed the iGEM project as part of our Masters dissertations at the University of Edinburgh. Due to academic requirements, the iGEM project, PhagED, was divided into individual sub-projects to allow the production of unique dissertations. Each of us completed a dissertation based on the work we did for iGEM, and these dissertations are available upon request.

Supervisors

Dr Elise Cachat

School of Biological Sciences

Academic supervisor.

Dr Heather Barker

School of Biological Sciences

Supervising lab work.

Holly Robertson-Dick

Industrial Liaison

Supervising iGEM administrative work.

Dr John White

School of Chemistry

Supervising phage work.

Dr Filippo Menolascina

School of Bioengineering

Supervising bacteria-phage modelling.

Dr Russell Brown

School of Biological Sciences

Supervising P1 phage construction.

Sponsors

We are very thankful to those who are helping us to make it happen.

FEEL FREE TO GET IN TOUCH

Contact