Difference between revisions of "Team:Munich/deletioncasette2.html"
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td> | + | <td>2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect.</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 14:43, 29 September 2018
Creating a selection cassette from pSB1C3
2018/08/28| Participants: | Dominic |
| Protocol: | PCR, Agarose Gel, Gelextraction |
| Notes: | Knockouts of both msb-B, RecBCD Primer with 50bp homology regions, Ta: 62°C, Elongation: 45s Expected: 1,1kb |
| Results: | 2 PCR reactions worked. One reaction for a resistance cassette flanked with FRT sites didnt work. Elena (Advisor) suggested that the template might be incorrect. |
