Difference between revisions of "Team:Munich/engineering2.html"
| Line 15: | Line 15: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td>MG1655 is E.Coli WT as backup | + | <td>Genetic engineering is planned in E. Coli Rosetta, MG1655 is E.Coli WT as backup. |
</td> | </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td>colonies only on MG1655 plate | + | <td>colonies only on E. Coli MG1655 plate; |
| − | No colonies on E. | + | No colonies on E. Coli Rosetta |
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | |||
| − | |||
<h4>pRED/ET genome engineering of delta msb-B and delta recBCD strains</h4> | <h4>pRED/ET genome engineering of delta msb-B and delta recBCD strains</h4> | ||
| + | <em>2018/08/29</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
| Line 46: | Line 45: | ||
<tr> | <tr> | ||
<td>Results:</td> | <td>Results:</td> | ||
| − | <td>red colonies | + | <td>red colonies. Elena (Advisor) and Dominic decided to repeat the experiment but to do a dpnI digest before to get rid of the template DNA. |
| − | + | ||
| − | + | ||
</td> | </td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
| − | |||
<h4>Transforming E. Coli DH5a to find a reason for the contamination</h4> | <h4>Transforming E. Coli DH5a to find a reason for the contamination</h4> | ||
| + | <em>2018/08/30</em> | ||
<table class="table table-borderless"> | <table class="table table-borderless"> | ||
<tr> | <tr> | ||
Revision as of 15:14, 29 September 2018
Transforming E. Coli Rosetta and MG1655 for pRED/ET engineering
2018/08/28
| Participants: | Dominic |
| Protocol: | epo trafo |
| Notes: | Genetic engineering is planned in E. Coli Rosetta, MG1655 is E.Coli WT as backup. |
| Results: | colonies only on E. Coli MG1655 plate;
No colonies on E. Coli Rosetta |
pRED/ET genome engineering of delta msb-B and delta recBCD strains
2018/08/29
| Participants: | Dominic |
| Protocol: | pRED/ET engineering protocol |
| Notes: | the template for the resistance cassette for deletions was taken from a plasmid containing mRFP |
| Results: | red colonies. Elena (Advisor) and Dominic decided to repeat the experiment but to do a dpnI digest before to get rid of the template DNA. |
Transforming E. Coli DH5a to find a reason for the contamination
2018/08/30
| Participants: | Dominic |
| Protocol: | epo trafo |
| Notes: | CAP_recBCD, CAP_msbb, psb1c3_mrfp in Dh5a |
| Results: | red colonies on all plates -> mRFP contamination |