Difference between revisions of "Team:Munich/GenomicDNA.html"
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<h4>genomic DNA extraction from E. Coli Rosetta</h4> | <h4>genomic DNA extraction from E. Coli Rosetta</h4> | ||
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td>Invitrogen purelink genomic DNA mini kit</td> | <td>Invitrogen purelink genomic DNA mini kit</td> | ||
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<h4>PCR amplification of RecB, C & D from E. Coli Rosetta</h4> | <h4>PCR amplification of RecB, C & D from E. Coli Rosetta</h4> | ||
| + | <em>2018/05/24</em> | ||
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<td>Protocol:</td> | <td>Protocol:</td> | ||
| − | <td>PCR</td> | + | <td>PCR, agarose gel, gel extraction</td> |
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<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td>RecC_5'UTR_extract_rv; RecC_3'UTR_extract_fw (AT:69°C) | + | <td>RecC: RecC_5'UTR_extract_rv; RecC_3'UTR_extract_fw (AT:69°C) <br> |
| − | RecB_extract_rv; RecD_extract_fw (AT: 69°C) | + | RecBCD: RecB_extract_rv; RecD_extract_fw (AT: 69°C) |
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</td> | </td> | ||
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<td>Results:</td> | <td>Results:</td> | ||
| − | <td>PIC | + | <td>PIC</td> |
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Revision as of 16:44, 29 September 2018
genomic DNA extraction from E. Coli Rosetta
2018/05/18| Participants: | Eni |
| Protocol: | Invitrogen purelink genomic DNA mini kit |
PCR amplification of RecB, C & D from E. Coli Rosetta
2018/05/24| Participants: | Eni |
| Protocol: | PCR, agarose gel, gel extraction |
| Notes: | RecC: RecC_5'UTR_extract_rv; RecC_3'UTR_extract_fw (AT:69°C) RecBCD: RecB_extract_rv; RecD_extract_fw (AT: 69°C) |
| Results: | PIC |