JimmyZhong (Talk | contribs) |
JimmyZhong (Talk | contribs) |
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+ | $(document).ready(function(){ | ||
+ | // Add smooth scrolling to all links | ||
+ | $("a").on('click', function(event) { | ||
+ | |||
+ | // Make sure this.hash has a value before overriding default behavior | ||
+ | if (this.hash !== "") { | ||
+ | // Prevent default anchor click behavior | ||
+ | event.preventDefault(); | ||
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+ | // Store hash | ||
+ | var hash = this.hash; | ||
+ | |||
+ | // Using jQuery's animate() method to add smooth page scroll | ||
+ | // The optional number (800) specifies the number of milliseconds it takes to scroll to the specified area | ||
+ | $('html, body').animate({ | ||
+ | scrollTop: $(hash).offset().top | ||
+ | }, 800, function(){ | ||
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+ | // Add hash (#) to URL when done scrolling (default click behavior) | ||
+ | window.location.hash = hash; | ||
+ | }); | ||
+ | } // End if | ||
+ | }); | ||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | <style> | ||
+ | body { | ||
+ | font-family: "Lato", sans-serif; | ||
+ | } | ||
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+ | .sidenav { | ||
+ | height: 80%; | ||
+ | width: 180px; | ||
+ | position: fixed; | ||
+ | z-index: 1; | ||
+ | top: 0; | ||
+ | left: 0; | ||
+ | background-color: #111; | ||
+ | overflow-x: hidden; | ||
+ | padding-top: 200px; | ||
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+ | .sidenav a { | ||
+ | padding: 5px 5px 5px 5px; | ||
+ | text-decoration: none; | ||
+ | font-size: 20px; | ||
+ | color: #818181; | ||
+ | display: block; | ||
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+ | .sidenav a:hover { | ||
+ | color: #f1f1f1; | ||
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+ | .main { | ||
+ | margin-left: 180px; /* Same as the width of the sidenav */ | ||
+ | padding: 0px 10px; | ||
+ | font-family: arial; | ||
+ | font-size: 20px; | ||
+ | padding: 30px | ||
+ | } | ||
+ | @media screen and (max-height: 450px) { | ||
+ | .sidenav {padding-top: 15px;} | ||
+ | .sidenav a {font-size: 18px;} | ||
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+ | |||
+ | |||
+ | <div class="sidenav"> | ||
+ | <a href="#graphicsum">Top</a> | ||
+ | <br> | ||
+ | <a href=#sum>Summary</a> | ||
+ | <br> | ||
+ | <a href=#1>Horseshoe Crabs</a> | ||
+ | <br> | ||
+ | <a href=#2>Current Practice</a> | ||
+ | <br> | ||
+ | <a href=#3>LAL and Factor C</a> | ||
+ | <br> | ||
+ | <a href=#4>Project Goal</a> | ||
+ | <br> | ||
+ | <a href=#5>Project Design</a> | ||
+ | <br> | ||
+ | <a href=#6>What's next</a> | ||
+ | <br> | ||
+ | <a href=#bibli>Bibliography</a> | ||
+ | </div> | ||
+ | <div class="graphicsum"> | ||
</div> | </div> | ||
<body> | <body> |
Revision as of 00:05, 30 September 2018
Build the change
You want to see in the world
Summary
Horseshoe Crabs
Current Practice
LAL and Factor C
Project Goal
Project Design
What's next
Bibliography
Construct design: The goal for our project is to insert Factor C gene into the chromosomal DNA of B. Subtilis, and have it express this Factor C protein. At the same time, to fulfill iGEM competition requirements, we also need to insert Factor C gene into E.coli plasmid pSB1C3 and submit it as a biobrick for iGEM competition. We decided to insert Factor C gene into E.coli plasmid pSB1C3 first; then, integrate Factor C gene into the chromosomal DNA of B. Subtilis using pAX01 integration factor (a B. Subtilis specific plasmid). Biobrick Factor C in E.coli pSB1C3 We couldn't order Factor C from factory because it is too large (about 3000 base pairs https://benchling.com/itesla-soundbio/f/gPUeHZXf-drafts/seq-Jq87odxl-psb1c3factor-c/edit), so we order it as two fragments, FC1 and FC2. First, insert FC1 into pSB1C3. Then, insert FC2 into the plasmid, too. This pSB1C3 with full Factor C will be submitted for biobrick. Integration Factor C in B. Subtilis Chromosomal DNA. We then digest full Factor C from Biobrick, and insert it into pAX01, a B. Subtilis integration factor. Then, we transform B. Subtilis with pAX01. After plasmid pAX01 enters B. Subtilis, there is a chance that it will integrate into chromosomal DNA. Expression Factor C protein extract B. Subtilis are used to express Factor C because they are gram positive and will not damage the Factor C protein. In contrast, E. coli is gram negative and will cleave FC protein. After B. Subtilis express and produce Factor C, we can then extract Factor C protein for further experiment.