Difference between revisions of "Team:HBUT-China/InterLab"
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| + | <p style="text-indent:25px; text-justify: inter-ideograph;text-align: justify;"> The goal of the iGEM InterLab Study is to identify and correct the sources of systematic variability in synthetic biology measurements, so that eventually, measurements that are taken in different labs will be no more variable than measurements taken within the same lab.</p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2018/8/85/T--HBUT-China--interlabPhoto.png" width="60%"> | ||
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| + | <p style="text-indent:25px; text-justify: inter-ideograph;text-align: justify;"> Because the fluorescence value measured by a plate reader is an aggregate measurement of an entire population of cells, we need to divide the total fluorescence by the number of cells in order to determine the mean expression level of GFP per cell. Usually we do this by measuring the absorbance of light at 600nm, from which we compute the “optical density (OD)” of the sample as an approximation of the number of cells. OD measurements are subject to high variability between labs, however, and it is unclear how good of an approximation an OD measurement actually is. If we used a more direct method to determine the cell count in each sample, then potentially we could remove another source of variability in our measurements. </p> | ||
| + | <h2>Report</h2> | ||
| + | <p>Please see this <a href="https://static.igem.org/mediawiki/2018/6/62/T--HBUT-China--interlabData.xlsx">Excel File</a> to view our raw and manipulated data.</p> | ||
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Latest revision as of 03:15, 30 September 2018
HBUT iGEM
The goal of the iGEM InterLab Study is to identify and correct the sources of systematic variability in synthetic biology measurements, so that eventually, measurements that are taken in different labs will be no more variable than measurements taken within the same lab. Because the fluorescence value measured by a plate reader is an aggregate measurement of an entire population of cells, we need to divide the total fluorescence by the number of cells in order to determine the mean expression level of GFP per cell. Usually we do this by measuring the absorbance of light at 600nm, from which we compute the “optical density (OD)” of the sample as an approximation of the number of cells. OD measurements are subject to high variability between labs, however, and it is unclear how good of an approximation an OD measurement actually is. If we used a more direct method to determine the cell count in each sample, then potentially we could remove another source of variability in our measurements. Please see this Excel File to view our raw and manipulated data.
InterLab
Report