Difference between revisions of "Team:Kyoto/Safety"

Line 62: Line 62:
 
   <h1>Safety</h1>
 
   <h1>Safety</h1>
 
   <ul class="material">
 
   <ul class="material">
−
       <li><a href="#Parts">1) Parts</a></li>
+
       <li><a href="#Safe project design">1) Safe project design</a></li>
−
       <li><a href="#Primer">2) Primer list</a></li>
+
       <li><a href="#">2) Safe lab work
−
       <li><a href="#Materials">3) Materials</a></li>
+
       <li><a href="#Safe shipment">3) Safe shipment</a></li>
−
        <ul>
+
      <li><a href="#Link">4) Link</a></li>
−
<li><a href="#Kit">3-1 Kit</a></li>
+
−
<li><a href="#Restriction Enzyme">3-2 Restriction Enzyme</a></li>
+
−
<li><a href="#Polymerase">3-3 Polymerase</a></li>
+
−
<li><a href="#DNA ligase">3-4 DNA ligase</a></li>
+
−
<li><a href="#Marker">3-5 Marker</a></li>
+
−
<li><a href="#Organism">3-6 Organism</a></li>
+
−
<li><a href="#Antibiotics">3-7 Antibiotics</a></li>
+
−
<li><a href="#Equipment">3-8 Equipment</a></li>
+
−
<li><a href="#Backbone">3-9 Backbone</a></li>
+
−
<li><a href="#Buffer">3-10 Buffer</a></li>
+
−
<li><a href="#SDS-PAGE&Westernblotting">3-11 SDS-PAGE&Westernblotting</a></li>
+
−
<!--------------------------------------------------------- BLOCK Contents-2 Materials  END----------------------------------------------------------->
+
 
         </ul>
 
         </ul>
−
 
 
−
  <li><a href="#methods">4) Methods</a></li>
 
−
    <ul>
 
−
 
−
<!--------------------------------------------------------- BLOCK Contents-3 Methods PY------------------------------------------------------------>
 
−
<li><a href="#Miniprep">4-1 Miniprep</a></li>
 
−
<li><a href="#Gel Extraction and PCR purification">4-2 Gel Extraction and PCR purification</a></li>
 
−
<li><a href="#Restriction Enzyme Digestion">4-3 Restriction Enzyme Digestion</a></li>
 
−
<li><a href="#Ligation">4-4 Ligation</a></li>
 
−
<li><a href="#Transformation">4-5 Transformation</a></li>
 
−
<!--------------------------------------------------------- BLOCK Contents-3 Methods  END------------------------------------------------------------>
 
 
   </ul>
 
   </ul>
 
</ul>
 
</ul>

Revision as of 08:08, 2 October 2018

Safety

1) Parts
Basic Parts
Composite Parts
2) Primer List
primer namesequenceLengthTmGC%DesignerManufacturer
tropomyosinFGATCGAGAAGGACAACGCCC206560FukudaMacrogen Japan Corp
IK107cccgccgccaccatggaggcggccgcaaaatcagg359471YoshimotoMacrogen Japan Corp
3) Materials
3-1 Kit
NameSupplier
Wizard® SV Gel and PCRPromega
FastGene™Plasmid Mini KitNIPPON Genetics Co.,Ltd
4) Methods
4-1 Miniprep

Minipreps were performed using FastGene™Plasmid Mini Kit and and Wizard® Plus SV Minipreps DNA Purification System according to the manufacturer's protocols.

4-17 Soaking

We followed this paper as for soaking.
https://link.springer.com/content/pdf/10.1007%2Fs10658-012-0035-0.pdf