Difference between revisions of "Team:SMMU-China/Composite Part"

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Revision as of 14:35, 3 October 2018

Composite part

We listed other composite part we constructed this year below.

BNP-AR185-eGFP-PloyA BBa_I712008
CMV-AR185-eGFP-PloyA BBa_I712006
BNP-eGFP-PloyA BBa_I712009
CMV-eGFP-PloyA BBa_I712010
L ITR-BNP-AR185-eGFP-PloyA-R ITR BBa_I712011
L ITR-CMV-AR185-eGFP-PloyA-R ITR BBa_I712012

Favorite composite part

BNP-AR185 T2A eGFP-polyA

For this year’s iGEM competition, our favorite composite part is BNP-AR185-polyA.This part is composed of BNP promotor, AR185 linked with eGFP by T2A and poly A.

How BNP-AR185-polyA works

As has shown presiously, BNP promoter activity is related to the severity of heart failure. So we want to use ET-1, one stimulus factor, to imitate the environment of heart failure and induce BNP promoter expression. Then, the later sequence of the part, AR185, also can be expressed and fuction what it could specifically bind to RyR2 in rat cardiomyocytes and have the ability to inhibit PKA dependent S2808 phosphorylation in vitro.

Fig.1 balablablabla

See More

Integrated information about AR185 and BNP promotor posted on Demonstrate page. Or you can alternatively check the (BBa_I712008) on the Registry of Standard Biological Parts website.

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