In the following table we present the parts we have used for our plasmid construction, detailed in Constructs, the parts we used are classified as they appear in our composite constructs:

For E: Coli

Table A. Strategy A: E. coli

(all optimized for E. coli)

Strategy B: E. coli

Simbology

Table B. Strategy B: E. coli

(all optimized for E. coli)

For L. rhamnosus GG:

Strategy A: L. rhamnosus GG

Simbology

Table C. Strategy A: L. rhamnosus GG

(all optimized for E. coli)

For L. rhamnosus GG:

Strategy B: L. rhamnosus GG

Simbology

Table D. Strategy B: L. rhamnosus GG

(all optimized for E. coli)

Signaling peptides

For E. Coli

Simbology

Table E. Sp1-Nsp4: E. coli

(all optimized for E. coli)

Sp2-Nsp2 (E. coli):

Simbology

Table F. Sp2-Nsp2: E. coli

(all optimized for E. coli)

Sp negative control (E. coli):

Simbology

Table G. Sp-negative control: E. coli

(all optimized for E. coli)

Sp positive control (E. coli):

Simbology

Table H. Sp positive control: E. coli

(all optimized for E. coli)

Sp1-Nsp4 (L.rhamnosus):

Simbology

Table I. Sp1-Nsp4: L. rhamnosus GG

(all optimized for E. coli)

Sp2-Nsp2 (L.rhamnosus):

Simbology

Table J. Sp2-Nsp2: L. rhamnosus GG

(all optimized for E. coli)