Difference between revisions of "Team:ECUST/InterLab"

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    <div id="bannerquote">Overview</div>
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    <div id="subbanner">ECUST 2018 </div>
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<h1 class="box-heading">Introduce</h1>
  
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<p>As one of the most important measurement marker in synthetic biology,however, the fluorescence data of GFP usually cannot be compared because it has been reported in different units or because different groups process data in different ways.</p>
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<p>The goal of the iGEM InterLab Study is to identify and correct the sources of systematic variability in synthetic biology measurements, so that eventually, measurements that are taken in different labs will be no more variable than measurements taken within the same lab,</p>
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<p>The goal for the fifth interlab is to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD. </p>
  
  
  
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<p> Highest average fluorescence was obtained from test device 5, closely followed by device 4 and test device 1.Test device 6 and test device 2 show a modest fluorescence intensity, while test devices 3 have any fluorescence signal as well as the negative group. The order of the promoters given by igem official website is, from strong to weak.test device 4,test device5,test deviece1,test device2,test device6,and test device3. The experimental value is in line with the expected value.</p>
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<h1>InterLab</h1>
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<p>Comparing the fluorescence values at 0h and 6h, it was found that the fluorescence value at 6h did not increase significantly. According to the given protocol from iGEM, the 0h bacterial liquid is not directly measured by fluorescence, but is measured after 6 hours of ice bath. We believe that the ice bath is not conducive to bacterial growth, so a large amount of fluorescent protein is expressed. The experiment confirmed our conjecture. If the 0h bacteria solution is directly measured instead of ice bath, the fluorescence value will be significantly lower than the fluorescence value of 6h.</p>
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<h3>Bronze Medal Criterion #4</h3>
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.  
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Revision as of 10:16, 6 October 2018

Overview
ECUST 2018

Introduce

As one of the most important measurement marker in synthetic biology,however, the fluorescence data of GFP usually cannot be compared because it has been reported in different units or because different groups process data in different ways.

The goal of the iGEM InterLab Study is to identify and correct the sources of systematic variability in synthetic biology measurements, so that eventually, measurements that are taken in different labs will be no more variable than measurements taken within the same lab,

The goal for the fifth interlab is to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD.

Discussion

Highest average fluorescence was obtained from test device 5, closely followed by device 4 and test device 1.Test device 6 and test device 2 show a modest fluorescence intensity, while test devices 3 have any fluorescence signal as well as the negative group. The order of the promoters given by igem official website is, from strong to weak.test device 4,test device5,test deviece1,test device2,test device6,and test device3. The experimental value is in line with the expected value.

Comparing the fluorescence values at 0h and 6h, it was found that the fluorescence value at 6h did not increase significantly. According to the given protocol from iGEM, the 0h bacterial liquid is not directly measured by fluorescence, but is measured after 6 hours of ice bath. We believe that the ice bath is not conducive to bacterial growth, so a large amount of fluorescent protein is expressed. The experiment confirmed our conjecture. If the 0h bacteria solution is directly measured instead of ice bath, the fluorescence value will be significantly lower than the fluorescence value of 6h.

Results