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| − | {{Valencia_UPV/bootstrap.css}}
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| − | {{Valencia_UPV/custom.css}}
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| − | {{Valencia_UPV/flickity.css}}
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| − | {{Valencia_UPV/iconsmind.css}}
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| − | {{Valencia_UPV/jquery.steps.css}}
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| − | {{Valencia_UPV/lightbox.min.css}}
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| − | {{Valencia_UPV/stackinterface.css}}
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| − | {{Valencia_UPV/theme.css}}
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| − | {{Valencia_UPV/navBar}}
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| | | | |
| − | <html>
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| − | <style>
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| − |
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| − | #home_logo, #sideMenu { display:none; }
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| − | #sideMenu, #top_title, .patrollink {display:none;}
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| − | #content { margin-left:0px; margin-top:-30px; padding:0px; width:100%;}
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| − |
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| − | </style>
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| − |
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| − | <head>
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| − | <meta charset="utf-8">
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| − | <title>Stack Multipurpose HTML Template</title>
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| − | <meta name="viewport" content="width=device-width, initial-scale=1.0">
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| − | <meta name="description" content="Site Description Here">
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| − | <link href="https://fonts.googleapis.com/css?family=Open+Sans:200,300,400,400i,500,600,700%7CMerriweather:300,300i" rel="stylesheet">
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| − | <link href="https://fonts.googleapis.com/icon?family=Material+Icons" rel="stylesheet">
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| − | <link href="https://fonts.googleapis.com/css?family=Lato:400,700,900" rel="stylesheet">
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| − | <link href="https://2018.igem.org/Template:Valencia_UPV/fontAwesome?action=raw&ctype=text/css" rel="stylesheet" type="text/css">
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| − |
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| − | </head>
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| − |
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| − | <body class="" style="background-color:#353535;">
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| − | <a id="start"></a>
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| − |
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| − | <!--end of row-->
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| − | </div>
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| − | <!--end of container-->
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| − | </nav>
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| − | </div>
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| − | <!--end bar-->
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| − | </div>
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| − | <div class="main-container">
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| − |
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| − |
| |
| − | <!-------------------------------IMAGEN PRINCIPAL------------------------------------------------>
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| − |
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| − |
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| − | <section style="
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| − | ; ; background-size: 100%;height: 44.2em;margin-top: 0em;padding-top: 1.1em;" class="height-90 parallax">
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| − | <div class="main-container" style="
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| − | height: 100%;">
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| − | <center style="
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| − | height: 100%;">
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| − | <div class="col-md-10" style="
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| − | height: 100%;margin-top: 4em;">
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| − | <div id="logoPrinteria" class="item" style="
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| − | background-image: url(https://static.igem.org/mediawiki/2018/3/3b/T--Valencia_UPV--laboratorioAdrianUPV2018.jpeg);
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| − | height: 100%;
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| − | width: 100%;background-attachment: fixed;background-size: cover;">
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| − |
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| − | <div style="/* position: absolute; *//* top: 81%; *//* padding-left: 31em; */">
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| − | <a class="btn down inner-link active" href="#story" style="font-size: 82%;right: 50%;/* position: fixed; *//* bottom: 7%; */top: 85.2%;z-index: 99;background-color: white;position: absolute;border-radius: 80%;width: 3.8em;height: 3.8em;padding: 0;padding-top: 14px;">
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| − | <i class="stack-interface stack-down-open-big"></i>
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| − | </a>
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| − | </div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − | </center>
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| − |
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| − | </div><div class="all-page-modals"></div><div class="all-page-modals"></div>
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| − | <div class="all-page-modals"></div>
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| − | </section></div>
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| − |
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| − |
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| − | <!----------------------EMPIEZA LA SECCIÓN DE DEBAJO DE LA IMAGEN ----------------------------->
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| − |
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| − |
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| − | <section id="story" class="feature-large" style="padding-top: 6em; padding-bottom: 50px; outline: none;" data-scroll-id="story" tabindex="-1">
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| − | <div class="container">
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| − | <div class="row">
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| − | <div class="col-md-3" style="
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| − | margin-left: 0em;
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| − | padding-right: 0px;
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| − | padding-left: 0.2em;
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| − | ">
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| − |
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| − | <!------------ÍNDICE LATERAL------------------>
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| − |
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| − | <div class="tabs-container tabs--vertical page-navigator" style="position: -webkit-sticky;position: sticky;padding-bottom: 0px;margin-top: 11.4em;">
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| − | <h4 style="
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| − | padding-left: 1em;
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| − | ">Index</h4>
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| − | <ul class="lateral">
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| − | <li class="lateral" style="margin-bottom: 0px;padding-bottom: 1em;padding-top: initial;padding-left: 1em;/* color: #353535; */">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | /* padding-left: 0.5em; */
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| − | ">
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| − | <a class="lateral" style="
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| − | /* color: #ffffff; */
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| − | opacity: 1;
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| − | list-style-type: circle;
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| − | ">INTRODUCTION</a>
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| − | </div>
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| − |
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a href="#buho" class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">STRUCTURE</a>
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| − | </div>
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| − |
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">ENTRY OF CONSUMABLES</a>
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| − | </div>
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| − |
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">REACTION ZONE</a>
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| − | </div>
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| − |
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">MEASUREMENT ZONE</a>
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| − | </div>
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| − |
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">DISINFECTION AND STERILITY</a>
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| − | </div>
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| − |
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| − | </li>
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">SIMULTANEOUS EXPERIMENTS</a>
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| − | </div>
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| − |
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| − | </li>
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| − | </li>
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| − | <li class="lateral">
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| − | <div class="tab__title" style="
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| − | line-height: 1.3em;
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| − | ">
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| − | <a class="lateral" style="
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| − | color: #353535;
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| − | opacity: 1;
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| − | ">RESULTS</a>
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| − | </div>
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| − |
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| − | </li>
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| − | </ul><ul class="tabs-content"></ul>
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| − |
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
| |
| − | <!--------------------------------------------TEXTO------------------------------------------------>
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| − |
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| − |
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| − | <div class="col-md-9" style="
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| − | /* margin-left: 0px; */
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| − | padding-left: 6em;
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| − | padding-right: 2em;
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| − | ">
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| − | <div>
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| − | <h3>HARDWARE</h3>
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| − | <h4>INTRODUCTION</h4>
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| − |
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| − | <p>To address the lack of accessibility to synthetic biology due to the high costs and the level of knowledge required among others, our team has designed Printeria, a fully-equipped bioengineering device capable of automating the process of printing genetic circuits in bacteria.
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| − | </p>
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| − |
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| − | <p> To ensure a functional, economical and easy-to-use design, collaboration between the hardware and the wet lab teams has been necessary throughout the development process. After several drafts we have achieved a unique design that can be easily transported and replicated at a cost not exceeding xxx € (xxx $). [and whose cost does not exceed xxx €]</p>
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| − |
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| − | <p> Printeria is divided into three main parts dependent on each other:</p>
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| − |
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| − | <div>
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| − | <ul>
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| − | <li>
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| − | <p> <u>Entry of consumables</u>: components that allow the entry of consumables into the reaction zone.</p>
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| − | </li>
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| − | <li>
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| − | <p> <u>Reaction zone</u>: space where the process of printing genetic circuits in bacteria occurs thanks to digital microfluidic technology.</p>
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| − | </li>
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| − | <li>
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| − | <p> <u>Measurement zone</u>: the bacteria are kept in conditions that allow for growth and expression while OD and fluorescence measurements are taken.</p>
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| − | </li>
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| − | </ul>
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| − | </div>
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| − | <div>
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| − | <img src="enlace de la imagen" alt="">
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| − |
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| − |
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| − | </div>
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| − |
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| − | <ul class="accordion accordion-1">
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| − | <li>
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| − | <div class="accordion__title">
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| − | <span class="h5">Overall budget 1</span>
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| − | </div>
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| − | <div class="accordion__content">
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| − | <div>
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| − | <table class="border--round table--alternate-row tableHec" style="width:100%;">
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| − | <thead><tr>
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| − | <th class="thHec">Part</th>
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| − | <th class="thHec">Quantity</th>
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| − | <th class="thHec">Cost per unit</th>
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| − | <th class="thHec">Cost</th>
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| − | </tr>
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| − | </thead><tbody><tr>
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| − | <td class="tdHec">Structure</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Entry system</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Reaction zone</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Measurement zone</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec" style="opacity: 0; border:none !important;"></td>
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| − | <td class="tdHec" style="opacity: 0; border:none !important;"></td>
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| − | <td class="tdHec" style="background-color: #909090; color: #fff">TOTAL</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | </tbody></table> </div>
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| − | </li>
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| − | <li>
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| − | <div class="accordion__title">
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| − | <span class="h5">Downloads 2</span>
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| − | </div>
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| − | <div class="accordion__content">
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| − | <div>
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| − | <ul>
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| − | <li>
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| − | <p> Assembly drawings</p>
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| − | </li>
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| − | <li>
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| − | <p> Printeria's CAD 3D model</p>
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| − | </li>
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| − | </ul>
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| − | </div>
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| − | </div>
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| − | </li>
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| − |
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| − | </ul>
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| − |
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| − |
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| − | <div>
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| − | <table class="border--round table--alternate-row tableHec" style="width:100%;">
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| − | <thead><tr>
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| − | <th class="thHec">Part</th>
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| − | <th class="thHec">Quantity</th>
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| − | <th class="thHec">Cost per unit</th>
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| − | <th class="thHec">Cost</th>
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| − | </tr>
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| − | </thead><tbody><tr>
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| − | <td class="tdHec">Structure</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Entry system</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Reaction zone</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Measurement zone</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec" style="opacity: 0; border:none !important;"></td>
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| − | <td class="tdHec" style="opacity: 0; border:none !important;"></td>
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| − | <td class="tdHec" style="background-color: #909090; color: #fff">TOTAL</td>
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| − | <td class="tdHec">----</td>
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| − | </tr>
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| − | </tbody></table>
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| − | </div>
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| − | <h5>STRUCTURE</h5>
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| − | <h5 style="padding-left: 1em;">Frame</h5>
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| − | <div style="padding-left: 1em;">
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| − | <p>First of all, it is necessary to design a structure that confines all the elements necessary for the operation of the device inside it and also allows the creation of a sterile atmosphere to carry out the biological reactions.</p>
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| − |
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| − | <p> In order to facilitate its transport, aluminum has been chosen for the frame, which in addition to its low weight, has other great qualities such as great resistance to corrosion and low cost.</p>
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| − |
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| − | <p> It has been used 15x15x1mm square tube profiles joined by plastic corner connectors obtaining as a result a 415x360x280mm chassis. Thanks to the generated blueprints we have been able to contact a company specializing in cutting and handling aluminum that has provided us with all the parts as we required. As it was not possible for us to find plastic connectors that fit our needs, we have decided to design these parts ourselves and then print them on a 3D printer. </p>
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| − |
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| − | <div>
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| − | <div class="row" style="
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| − | margin: 0;
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| − | ">
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| − | <div class="col-md-6" style="
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| − | padding: 0;
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| − | padding-right: 0.3em;
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| − | ">
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| − | <img src="https://via.placeholder.com/800x400">
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| − | </div>
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| − | <div class="col-md-6" style="
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| − | padding: 0;
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| − | padding-left: 0.3em;
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| − | ">
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| − | <img src="https://via.placeholder.com/800x400">
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| − | </div>
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| − | <div style="
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| − | width: 100%;
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| − | padding-left: 19em;
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| − | padding-right: 1em;
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| − | "><h6>Aluminum frame</h6>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
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| − | <h5 style="padding-left: 1em;">Methacrylate</h5>
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| − |
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| − | <div style="padding-left: 1em;">
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| − | <p> To complete the structure, it is necessary to create walls that are attached to this frame and contribute to its stability. We have opted for the use of methacrylate due to its attractive appearance, durability and scratch resistance. </p>
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| − |
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| − | <p> As well as the aluminum profiles, the blueprints created were used for ordering the custom-made parts to a company.</p>
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| − |
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| − | <div style="
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| − | padding-bottom: 1em;
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| − | text-align: right;
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| − | ">
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| − | <img src="https://via.placeholder.com/800x400" alt="" style="
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| − | margin-top: 2.8em;
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| − | margin-bottom: 0.8em;
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| − | border-radius: 0.3em;
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| − | ">
| |
| − | <div style="
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| − | width: 100%;
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| − | padding-left: 19em;
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| − | padding-right: 1em;
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| − | "><h6>Methacrylate cladding</h6>
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| − | </div>
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| − | </div>
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| − |
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| − | <p> Finally, 4mm diameter countersunk head screws DIN 7991 (M4 L25) and nuts DIN 934 (M4), both made of galvanized steel, were used for joining the methacrylate plates to the frame. </p>
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| − |
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| − | </div>
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| − |
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| − | <h5 style="padding-left: 1em;">Leveling system</h5>
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| − |
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| − | <div style="padding-left: 1em;">
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| − | <p> Given the great importance of keeping the reaction zone perfectly horizontal, it is necessary to use levelling feet. In addition, for checking the horizontal position, a bull’s eye level has been installed next to the reaction zone. </p>
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| − |
| |
| − | <div style="
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| − | padding-bottom: 0.8em;
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| − | text-align: right;
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| − |
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| − | ">
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| − | <div class="row" style="margin: 0;">
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| − | <div class="col-md-6" style="
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| − | padding: 0;
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| − | padding-right: 0.3em;
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| − | ">
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| − | <img src="https://via.placeholder.com/800x400" alt="" style="
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| − | margin-top: 1.8em;
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| − | margin-bottom: 0.8em;
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| − | border-radius: 0.3em;
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| − | ">
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| − | </div>
| |
| − | <div class="col-md-6" style="
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| − | padding: 0;
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| − | padding-left: 0.3em;
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| − | ">
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| − | <img src="https://via.placeholder.com/800x400" alt="" style="
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| − | margin-top: 1.8em;
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| − | margin-bottom: 0.8em;
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| − | border-radius: 0.3em;
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| − | ">
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| − | </div>
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| − | <div style="
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| − | width: 100%;
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| − | padding-left: 19em;
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| − | padding-right: 1em;
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| − | "><h6>Levelling system</h6>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − |
| |
| − | <div>
| |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%;">
| |
| − | <thead><tr>
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| − | <th class="thHec">Part</th>
| |
| − | <th class="thHec">Quantity</th>
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| − | <th class="thHec">Cost per unit</th>
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| − | <th class="thHec">Cost</th>
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| − | </tr>
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| − | </thead><tbody><tr>
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| − | <td class="tdHec">Aluminum profiles</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
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| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Profiles' cutting and drilling</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Custom-made methacrylate</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Screws DIN 7991</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Nuts DIN 934</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Kg of PLA for the connectors</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Leeveling feet</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec">Bull's eye level</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | <tr>
| |
| − | <td class="tdHec" style="opacity: 0; border:none !important;"></td>
| |
| − | <td class="tdHec" style="opacity: 0; border:none !important;"></td>
| |
| − | <td class="tdHec" style="background-color: #909090; color: #fff">TOTAL</td>
| |
| − | <td class="tdHec">----</td>
| |
| − | </tr>
| |
| − | </tbody></table>
| |
| − |
| |
| − | </div>
| |
| − |
| |
| − | <h5>ENTRY OF CONSUMABLES</h5>
| |
| − |
| |
| − | </div>
| |
| − | <h5>REACTION ZONE</h5>
| |
| − |
| |
| − | <h5>MEASUREMENT ZONE</h5>
| |
| − |
| |
| − | <h5>DISINFECTION AND STERILITY</h5>
| |
| − |
| |
| − | <h5>SIMULTANEOUS EXPERIMENTS</h5>
| |
| − |
| |
| − | <h5>RESULTS</h5>
| |
| − |
| |
| − |
| |
| − |
| |
| − | </div>
| |
| − | </div>
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| − |
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| − |
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| − | </section>
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| − |
| |
| − |
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| − | <!--------------------FIN TEXTO------------------------------>
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