Difference between revisions of "Team:Ecuador/Model"

Line 1: Line 1:
 
{{Ecuador}}
 
{{Ecuador}}
 
<html>
 
<html>
−
<style>
+
<head>
−
    @import url('https://fonts.googleapis.com/css?family=Raleway');
+
<meta charset="utf-8">
−
    *{
+
<title>C-lastin, Modeling</title>
−
        margin: 0px;
+
<link rel="stylesheet" type="text/css" href="ecu--template.css">
−
        font-family: 'Raleway', sans-serif;
+
<link rel="stylesheet" type="text/css" href="ecu--modeling.css">
−
    }
+
</head>
−
  @keyframes slide-in {
+
−
    from {
+
−
      margin-left: -100%;
+
−
      border-bottom-right-radius: 30px;
+
−
      border-top-right-radius: 30px;
+
−
    }
+
  
−
    to {
+
<body>
−
      margin-left: 0%;
+
<div class="ecuador_menu">
−
      border-bottom-right-radius: 0;
+
−
      border-top-right-radius: 0px;
+
−
    }
+
−
  }
+
−
 
+
−
  @keyframes slide-in-menu {
+
−
    from {
+
−
      margin-left: -120%;
+
−
      border-bottom-right-radius: 30px;
+
−
      border-top-right-radius: 30px;
+
−
      opacity: 0.5;
+
−
    }
+
−
 
+
−
    to {
+
−
      margin-left: 0%;
+
−
      border-bottom-right-radius: 0;
+
−
      border-top-right-radius: 0px;
+
−
      opacity: 1;
+
−
    }
+
−
  }
+
−
 
+
−
  @keyframes fade-in {
+
−
    from {
+
−
      opacity: 0;
+
−
    }
+
−
 
+
−
    to {
+
−
      opacity: 1;
+
−
    }
+
−
  }
+
−
 
+
−
  @keyframes slide-in-oposite {
+
−
    from {
+
−
      margin-left: 100%;
+
−
    }
+
−
 
+
−
    to {
+
−
      margin-left: 0%;
+
−
    }
+
−
  }
+
−
 
+
−
  @keyframes slide-in-up {
+
−
    from {
+
−
      margin-top: 100%;
+
−
    }
+
−
 
+
−
    to {
+
−
      margin-left: 0%;
+
−
    }
+
−
  }
+
−
 
+
−
  @keyframes slide-in-up-oposite {
+
−
    from {
+
−
      margin-top: -100%;
+
−
    }
+
−
 
+
−
    to {
+
−
      margin-left: 0%;
+
−
    }
+
−
  }
+
−
  .igem_2018_team_menu.displaying_menu {
+
−
    display: none;
+
−
  }
+
−
  .ecuador_menu {
+
−
    font-size: 14px;
+
−
    width: 100%;
+
−
    height: 80px;
+
−
    position: fixed;
+
−
    left: 0px;
+
−
    top: 15px;
+
−
    background-color: #2471A3;
+
−
    animation-name: fade-in;
+
−
    animation-duration: 2s;
+
−
    -webkit-box-shadow: 0px 3px 11px 0px rgba(0,0,0,0.75);
+
−
    -moz-box-shadow: 0px 3px 11px 0px rgba(0,0,0,0.75);
+
−
    box-shadow: 0px 3px 11px 0px rgba(0,0,0,0.75);
+
−
    display: flex;
+
−
    z-index: 5;
+
−
  }
+
−
 
+
−
  .ecuador_home {
+
−
    width: 16%;
+
−
    height: 100%;
+
−
    display: flex;
+
−
    justify-content: center;
+
−
    background-color: #1F618D;
+
−
    position: absolute;
+
−
    align-items: center;
+
−
    margin: auto auto;
+
−
    left: 1.5%;
+
−
    color: #FFF;
+
−
    transition: 0.5s;
+
−
    -webkit-box-shadow: inset -50px -42px 114px -30px rgba(0,0,0,0.75);
+
−
    -moz-box-shadow: inset -50px -42px 114px -30px rgba(0,0,0,0.75);
+
−
    box-shadow: inset -50px -42px 114px -30px rgba(0,0,0,0.75);
+
−
    z-index: 10;
+
−
  }
+
−
 
+
−
  .ecuador_home:hover {
+
−
    cursor: pointer;
+
−
    font-size: 16px;
+
−
    background-color: #1A5276;
+
−
    box-shadow: none;
+
−
  }
+
−
 
+
−
  .menu_options_container {
+
−
    width: 79%;
+
−
    height: 50px;
+
−
    position: absolute;
+
−
    left: 19%;
+
−
    top: 15px;
+
−
    display: flex;
+
−
    justify-content: center;
+
−
  }
+
−
 
+
−
  .menu_option {
+
−
    width: 14.28%;
+
−
    height: 100%;
+
−
    position: relative;
+
−
    justify-content: center;
+
−
    align-items: center;
+
−
    display: flex;
+
−
    color: #FFFFFF;
+
−
    transition: 0.2s;
+
−
    text-align: center;
+
−
  }
+
−
 
+
−
  #project {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 0.5s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 0%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  #parts {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 0.6s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 12.5%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  #human_practice {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 0.7s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 25%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  #safety {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 0.8s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 37.5%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  #interlab {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 0.9s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 50%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  #judging_forms {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 1s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 62.5%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  #team {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 1.1s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 75%;
+
−
    z-index: 9;
+
−
  }
+
−
#atributions {
+
−
    animation-name: slide-in-up-oposite;
+
−
    animation-duration: 0.5s;
+
−
    animation-delay: 1.2s;
+
−
    animation-fill-mode: backwards;
+
−
    position: absolute;
+
−
    left: 87.5%;
+
−
    z-index: 9;
+
−
  }
+
−
 
+
−
  .ecuador_href_text {
+
−
    width: 14.28% !important;
+
−
    height: 100% !important;
+
−
    position: relative !important;
+
−
    justify-content: center !important;
+
−
    align-items: center !important;
+
−
    display: flex !important;
+
−
    color: #FFFFFF !important;
+
−
    transition: 0.2s !important;
+
−
    text-align: center !important;
+
−
    text-decoration: none !important;
+
−
    font-weight: bold !important;
+
−
  }
+
−
 
+
−
  .ecuador_href_sub_text {
+
−
    width: 100% !important;
+
−
    height: 40px !important;
+
−
    position: relative !important;
+
−
    text-align: left !important;
+
−
    text-indent: 20px !important;
+
−
    display: flex !important;
+
−
    background-color: #000 !important;
+
−
    margin: auto auto !important;
+
−
    align-items: center !important;
+
−
    transition: 0.2s !important;
+
−
    color: #D5DBDB !important;
+
−
    text-decoration: none !important;
+
−
  }
+
−
 
+
−
  .menu_option:hover {
+
−
    margin-top: 3px;
+
−
    cursor: pointer;
+
−
  }
+
−
 
+
−
  .menu_option:hover div.menu_sub_options_container{
+
−
    display: block;
+
−
  }
+
−
 
+
−
  .menu_sub_options_container {
+
−
    width: 100%;
+
−
    height: auto;
+
−
    position: absolute;
+
−
    top: 50px;
+
−
    display: none;
+
−
  }
+
−
 
+
−
  .sub_menu_option {
+
−
    width: 100%;
+
−
    height: 40px;
+
−
    position: relative;
+
−
    text-align: left;
+
−
    text-indent: 20px;
+
−
    display: flex;
+
−
    background-color: #000;
+
−
    margin: auto auto;
+
−
    align-items: center;
+
−
    transition: 0.2s;
+
−
    color: #D5DBDB;
+
−
  }
+
−
 
+
−
  .sub_menu_option:hover {
+
−
    text-indent: 15px;
+
−
    background-color: #2E86C1;
+
−
  }
+
−
 
+
−
  .ecuador_footer {
+
−
    width: 100%;
+
−
      height: 250px;
+
−
      position: relative;
+
−
      left: 0px;
+
−
      background-color: #212F3C;
+
−
      top: 500px;
+
−
  }
+
−
 
+
−
  .ecuador_footer_igem_logo {
+
−
      width: 180px;
+
−
      height: 180px;
+
−
      position: absolute;
+
−
      right: 80px;
+
−
      top: 35px;
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/5/55/T--Ecuador--iGEM--Logo--Black.png);
+
−
      background-size: 100%;
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
    }
+
−
 
+
−
    .ecuador_footer_information_layout {
+
−
      width: 75%;
+
−
      height: 80%;
+
−
      position: absolute;
+
−
      left: 60px;
+
−
      top: 15%;
+
−
      display: flex;
+
−
    }
+
−
 
+
−
    .ecuador_footer_information_container {
+
−
      width: 33.33%;
+
−
      height: 100%;
+
−
      position: relative;
+
−
    }
+
−
 
+
−
    .ecuador_footer_information_title_container {
+
−
      width: 100%;
+
−
      height: 25px;
+
−
      position: relative;
+
−
      top: 0px;
+
−
      text-align: center;
+
−
      font-size: 20px;
+
−
      font-weight: bold;
+
−
      color: #B2BABB;
+
−
    }
+
−
 
+
−
    .ecuador_follow_us_icons_container {
+
−
width: 50%;
+
−
height: 60px;
+
−
position: relative;
+
−
top: 40px;
+
−
margin: auto;
+
−
display: flex;
+
−
    }
+
−
.ecuador_information_data_container{
+
−
width: 50%;
+
−
      height: 60px;
+
−
      position: relative;
+
−
      top: 55px;
+
−
      margin: auto;
+
−
      display: flex;
+
−
}
+
−
 
+
−
    .ecuador_follow_us_icon {
+
−
      width: 70px;
+
−
      height: 60px;
+
−
      position: relative;
+
−
      margin: auto;
+
−
    }
+
−
 
+
−
    #facebook {
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/4/42/T--Ecuador--Facebook.png);
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
      background-size: 80%;
+
−
      transition: 0.4s;
+
−
      border-radius: 5px;
+
−
    }
+
−
 
+
−
    #facebook:hover {
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/4/40/T--Ecuador--Facebook--White.png);
+
−
      cursor: pointer;
+
−
    }
+
−
 
+
−
    #instagram {
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/9/90/T--Ecuador--Instagram.png);
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
      background-size: 80%;
+
−
      transition: 0.4s;
+
−
      border-radius: 5px;
+
−
    }
+
−
 
+
−
    #instagram:hover {
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/4/49/T--Ecuador--Instagram--White.png);
+
−
      cursor: pointer;
+
−
    }
+
−
.ecuador_sponsors_icons_container {
+
−
width: 100%;
+
−
height: 60px;
+
−
position: relative;
+
−
top: 40px;
+
−
margin-top: auto;
+
−
margin-right: auto;
+
−
margin-left: auto;
+
−
margin-bottom: auto;
+
−
display: flex;
+
−
    }
+
−
.ecuador_sponsors_icon {
+
−
width: 60px;
+
−
height: 60px;
+
−
position: relative;
+
−
margin-right: 15px;
+
−
margin-left: 15px;
+
−
    }
+
−
#ing_bio{
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/1/17/T--Ecuador--Logo--BIO.png/902px-T--Ecuador--Logo--BIO.png);
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
      background-size: 90%;
+
−
      transition: 0.4s;
+
−
      border-radius: 5px;
+
−
    }
+
−
#ing_bio:hover {
+
−
      background-size: 93%;
+
−
      cursor: pointer;
+
−
    }
+
−
#espe{
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/d/d2/T--Ecuador--Logo--ESPE.png/1066px-T--Ecuador--Logo--ESPE.png);
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
      background-size: 120%;
+
−
      transition: 0.4s;
+
−
      border-radius: 5px;
+
−
    }
+
−
#espe:hover {
+
−
      background-size: 125%;
+
−
      cursor: pointer;
+
−
    }
+
−
#idgen{
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/e/e2/T--Ecuador--Logo--Idgen.png);
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
      background-size: 90%;
+
−
      transition: 0.4s;
+
−
      border-radius: 5px;
+
−
    }
+
−
#idgen:hover {
+
−
      background-size: 93%;
+
−
      cursor: pointer;
+
−
    }
+
−
#tukii{
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/2/26/T--Ecuador--Logo--Tukii.png/1024px-T--Ecuador--Logo--Tukii.png);
+
−
      background-repeat: no-repeat;
+
−
      background-position: center;
+
−
      background-size: 90%;
+
−
      transition: 0.4s;
+
−
      border-radius: 5px;
+
−
    }
+
−
#tukii:hover {
+
−
      background-size: 93%;
+
−
      cursor: pointer;
+
−
    }
+
−
 
+
−
  .ecuador_abstract_container {
+
−
      width: 100%;
+
−
      height: 700px;
+
−
      position: absolute;
+
−
      left: 0px;
+
−
      top: 110px;
+
−
      background-color: #21618C;
+
−
    }
+
−
 
+
−
    .ecuador_abstract_title_cotainer {
+
−
      width: 100%;
+
−
      height: 50px;
+
−
      position: relative;
+
−
      top: 30px;
+
−
bottom: 30px;
+
−
      margin: auto;
+
−
      color: #FFF;
+
−
      font-size: 32px;
+
−
      display: flex;
+
−
      justify-content: center;
+
−
      align-items: center;
+
−
      animation-name: slide-in;
+
−
      animation-duration: 1.5s;
+
−
    }
+
−
.ecuador_img_descrip{
+
−
width: 100%;
+
−
      height: 35%;
+
−
      position: relative;
+
−
      left: 0px;
+
−
      top: 40px;
+
−
      margin-top: 10px;
+
−
      background-color: #FFF;
+
−
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/c/cb/T--Ecuador--ABSTRACT--001.jpeg/1200px-T--Ecuador--ABSTRACT--001.jpeg);
+
−
      background-position: center;
+
−
      background-repeat: no-repeat;
+
−
      background-size: 45%;
+
−
      animation-name: fade-in;
+
−
      animation-duration: 4s;
+
−
}
+
−
 
+
−
    .ecuador_abstract_text_container {
+
−
      width: 100%;
+
−
      height: 100px;
+
−
      top: 40px;
+
−
      position: relative;
+
−
    }
+
−
 
+
−
    .ecuador_abstract_text {
+
−
      width: 60%;
+
−
      height: 100%;
+
−
      position: relative;
+
−
      margin: auto;
+
−
      background-color: #21618C;
+
−
      border-radius: 20px;
+
−
      color: #FFF;
+
−
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iGEM ECUADOR
 
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<li><a href="#WWD">WHAT DID WE DO?</a></li>
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<li><a href="#HWD">HOW DID WE DO IT?</a></li>
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<li><a href="#RES">RESULTS</a></li>
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<li><a href="#EXP">EXPERIENCE</a></li>
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<li><a href="#ACK">ACKNOWLEDGEMENT</a></li>
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        About our model.
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<h2><a name="WWD">WHAT DID WE DO?</a></h2>
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  <p>Parrafo<br></p>
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  <div class="ec--img--wwd--cont">
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<p><img width="700" height="500" src="recursos extras/modeling.jpeg" alt="Transfected with GFP (Green Fluorescent Protein) Recorded using a 63x objective 3 days in vitro mouse Made by Jurjen Broeke"></p>
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          <h3>Converting between  absorbance of cells to absorbance of a known concentration of beads.</h3>
−
 
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              <p>...</p>
−
  <div class="ecuador_footer">
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              <h3>Counting  colony-forming units (CFUs) from the sample.</h3>
−
    <div class="ecuador_footer_igem_logo">
+
<p>…</p>
−
 
+
  <h2><a name="HWD">HOW DID WE DO IT?</a></h2>
−
      </div>
+
<p>In order to avoid distortions in the results due to  differences in the experimentation processes, we follow the procedures  described in the iGEM protocol (<a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf">Plate reader protocol</a>, <a href="http://parts.igem.org/Help:Protocols/Competent_Cells">component_cells</a>,  <a href="http://parts.igem.org/Help:Protocols/Transformation">Transformation</a>)
−
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    <table border="0" cellspacing="8px" cellpadding="0">
 +
          <tr>
 +
            <td width="148" valign="top"><p ><strong>Device</strong><strong> </strong></p></td>
 +
            <td width="132" valign="top"><p ><strong>Part Number</strong><strong> </strong></p></td>
 +
            <td width="104" valign="top"><p ><strong>Location</strong><strong> </strong></p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-neg-c">Negative control</p></td>
 +
            <td width="132" valign="top"><p>BBa_R0040 </p></td>
 +
            <td width="104" valign="top"><p>Well 2D </p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="type">Positive control </p></td>
 +
            <td width="132" valign="top"><p>BBa_I20270 </p></td>
 +
            <td width="104" valign="top"><p>Well 2B</p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-td1">Test Device 1 </p></td>
 +
            <td width="132" valign="top"><p>BBa_J364000 </p></td>
 +
            <td width="104" valign="top"><p>Well 2F    &nbsp; </p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-td2">Test Device 2 </p></td>
 +
            <td width="132" valign="top"><p>BBa_J364001 </p></td>
 +
            <td width="104" valign="top"><p>Well 2H    &nbsp; </p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-td3">Test Device 3 </p></td>
 +
            <td width="132" valign="top"><p>BBa_J364002 </p></td>
 +
            <td width="104" valign="top"><p>Well 2J </p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-td4">Test Device 4 </p></td>
 +
            <td width="132" valign="top"><p>BBa_J364007 </p></td>
 +
            <td width="104" valign="top"><p>Well 2L    &nbsp; </p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-td5">Test Device 5 </p></td>
 +
            <td width="132" valign="top"><p>BBa_J364008 </p></td>
 +
            <td width="104" valign="top"><p>Well 2N </p></td>
 +
              </tr>
 +
          <tr>
 +
            <td width="148" valign="top"><p id="label-td6">Test Device 6 </p></td>
 +
            <td width="132" valign="top"><p>BBa_J364009 </p></td>
 +
            <td width="104" valign="top"><p>Well 2P    &nbsp; </p></td>
 +
              </tr>
 +
            </table>
 +
  </div>
 +
<div id="rightBox">
 +
<div class="ec--biobr--location--container">
 +
<div class="loc-neg-c">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="359" height="75" src="modeling_6_oct_clip_image002.png"> </p>
 +
<br>
 +
<p>length: 54 bp </p>
 +
</div>
 +
<div class="textdis" id="pos_c">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="195" height="70" src="modeling_6_oct_clip_image003.png"></p>
 +
<br>
 +
<p>length: 919 bp</p>
 +
</div>
 +
<div class="loc-td1">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="195" height="51" src="modeling_6_oct_clip_image004.png" > </p>
 +
<br>
 +
<p>length: 918 bp </p>
 +
</div>
 +
<div class="loc-td2">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="189" height="54" src="modeling_6_oct_clip_image005.png" ></p>
 +
<br>
 +
<p>length: 918 bp</p>
 +
</div>
 +
<div class="loc-td3">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="197" height="63" src="modeling_6_oct_clip_image006.png" > </p>
 +
<br>
 +
<p>length: 918 bp </p>
 +
</div>
 +
<div class="loc-td4">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="196" height="52" src="modeling_6_oct_clip_image007.png"></p>
 +
<br>
 +
<p>length: 918 bp</p>
 +
</div>
 +
<div class="loc-td5">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="188" height="64" src="modeling_6_oct_clip_image008.png"  > </p>
 +
<br>
 +
<p>length: 918 bp </p>
 +
</div>
 +
<div class="loc-td6">
 +
<p><strong>Location</strong></p><br>
 +
<p><img width="194" height="55" src="modeling_6_oct_clip_image009.png"></p>
 +
<br>
 +
<p>length: 918 bp</p>
 +
</div>
 +
  </div>
 +
</div>
 +
  </div>
 
</div>
 
</div>
−
</a>
+
<h2><a name="RES">RESULTS</a></h2>
−
            </div>
+
              <strong>Particle standard curves</strong>
−
          </div>
+
  <div class="ec--img--wwd--cont">
−
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m.me/BiosinEcuador
+
              <p><strong>Fluorescein standard curves</strong> </p>
 +
              <div class="ec--img--wwd--cont">
 +
    <p><img src="recursos extras/A-FSC.1.png" width="779" height="445" alt=""/><br></p>
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              <div class="ec--img--wwd--cont">
 +
  <img src="recursos extras/A-FSC.2.png" width="779" height="445" alt=""/><br>
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                <strong>Net  Absorbance 600 as number of particles/100 uL</strong></p>
 +
<div class="ec--img--wwd--cont">
 +
              <p><img src="recursos extras/image019.png" width="752" height="452" alt=""/></p>
 
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<h2><a name="EXP">EXPERIENCE</a></h2>
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              <p><img src="https://svgsilh.com/svg/2324013-2196f3.svg" width="752" height="452" alt=""/></p>
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</div>
 +
              <p><em>As a team the interlab  challenge was useful&nbsp;because we learn about the protocols and the handling  of the plate reader and how it can be used in later processes of our project</em></p>
 +
  <br>
 +
<h2><a name="ACK">ACKNOWLEDGEMENT</a></h2>
 +
  <br>
 +
                As a team, we would like to thank the  Biomedicine team of Universidad Tecnica Equinoccial and especially Linda  Guaman, member of their research team, for allowing us to use their measurement  equipment, which were essential for the completion of the Interlab.</div>
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Revision as of 03:56, 7 October 2018

iGEM ECUADOR
C-lastin, Modeling
iGEM ECUADOR

Modeling

Reference image

WHAT DID WE DO?

Parrafo

Transfected with GFP (Green Fluorescent Protein) Recorded using a 63x objective 3 days in vitro mouse Made by Jurjen Broeke

Converting between absorbance of cells to absorbance of a known concentration of beads.

...

Counting colony-forming units (CFUs) from the sample.

…

HOW DID WE DO IT?

In order to avoid distortions in the results due to differences in the experimentation processes, we follow the procedures described in the iGEM protocol (Plate reader protocol, component_cells, Transformation)


BIOBRICKS


Device

Part Number

Location

Negative control

BBa_R0040

Well 2D

Positive control

BBa_I20270

Well 2B

Test Device 1

BBa_J364000

Well 2F  

Test Device 2

BBa_J364001

Well 2H  

Test Device 3

BBa_J364002

Well 2J

Test Device 4

BBa_J364007

Well 2L  

Test Device 5

BBa_J364008

Well 2N

Test Device 6

BBa_J364009

Well 2P  

Location



length: 54 bp

Location



length: 919 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

RESULTS

Particle standard curves


Fluorescein standard curves



Net Absorbance 600 as number of particles/100 uL

EXPERIENCE

As a team the interlab challenge was useful because we learn about the protocols and the handling of the plate reader and how it can be used in later processes of our project


ACKNOWLEDGEMENT


As a team, we would like to thank the Biomedicine team of Universidad Tecnica Equinoccial and especially Linda Guaman, member of their research team, for allowing us to use their measurement equipment, which were essential for the completion of the Interlab.
Quote
“Research from the middle of the world”
Sponsoring
Follow us on
Fb & Inst
Contact us
biosin.espe@outlook.es
UNIVERSIDAD DE LAS FUERZAS ARMADAS ESPE.
Ecuador,Sangolquí, Av. General Rumiñahui s/n y Ambato