Difference between revisions of "Team:Ecuador/Model"

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<title>C-lastin, Modeling</title>
 
<title>C-lastin, Modeling</title>
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</head>
 
</head>
  

Revision as of 03:56, 7 October 2018

iGEM ECUADOR
C-lastin, Modeling
iGEM ECUADOR

Modeling

Reference image

WHAT DID WE DO?

Parrafo

Transfected with GFP (Green Fluorescent Protein) Recorded using a 63x objective 3 days in vitro mouse Made by Jurjen Broeke

Converting between absorbance of cells to absorbance of a known concentration of beads.

...

Counting colony-forming units (CFUs) from the sample.

…

HOW DID WE DO IT?

In order to avoid distortions in the results due to differences in the experimentation processes, we follow the procedures described in the iGEM protocol (Plate reader protocol, component_cells, Transformation)


BIOBRICKS


Device

Part Number

Location

Negative control

BBa_R0040

Well 2D

Positive control

BBa_I20270

Well 2B

Test Device 1

BBa_J364000

Well 2F  

Test Device 2

BBa_J364001

Well 2H  

Test Device 3

BBa_J364002

Well 2J

Test Device 4

BBa_J364007

Well 2L  

Test Device 5

BBa_J364008

Well 2N

Test Device 6

BBa_J364009

Well 2P  

Location



length: 54 bp

Location



length: 919 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

Location



length: 918 bp

RESULTS

Particle standard curves


Fluorescein standard curves



Net Absorbance 600 as number of particles/100 uL

EXPERIENCE

As a team the interlab challenge was useful because we learn about the protocols and the handling of the plate reader and how it can be used in later processes of our project


ACKNOWLEDGEMENT


As a team, we would like to thank the Biomedicine team of Universidad Tecnica Equinoccial and especially Linda Guaman, member of their research team, for allowing us to use their measurement equipment, which were essential for the completion of the Interlab.
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UNIVERSIDAD DE LAS FUERZAS ARMADAS ESPE.
Ecuador,Sangolquí, Av. General Rumiñahui s/n y Ambato