Difference between revisions of "Team:AHUT China/Attributions"

Line 1: Line 1:
 
{{:Team:AHUT_China/Template/CSS_RESET}}
 
{{:Team:AHUT_China/Template/CSS_RESET}}
−
<html lang="en">
+
{{Heidelberg/main|
−
    <head>
+
    Attributions|
−
        <!-- Required meta tags -->
+
     We want to say thanks|
−
        <meta charset="utf-8">
+
     https://static.igem.org/mediawiki/2017/d/df/MS_Neural_Network_Brain_Gaia_Background.png|red|
−
        <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no">
+
    {{Heidelberg/templateus/Contentsection|
−
        <link rel="icon" href="image/favicon.png" type="image/png">
+
        {{#tag:html|
−
        <title>Royal Hotel</title>
+
             Here we want to thank everyone who supported us this year and thus helped us make our project come true. Numerous individuals, research groups, departments and companies supported us throughout the entire project and here we want to say thank you for this!
−
        <!-- Bootstrap CSS -->
+
<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/bootstrap_css?action=raw&ctype=text/css" type="text/css" />
+
Our iGEM team is from Anhui University of Technology, Ma 'anshan, China.
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/v_style_css?action=raw&ctype=text/css" type="text/css" />
+
The name of our project this year is Carbon dioxide purifier. The brief introduction of the project is as follows: Our iGEM team is from Anhui University of Technology, Ma 'anshan, China.<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/font-awesomemin_css?action=raw&ctype=text/css" type="text/css" />
+
The name of our project this year is Carbon dioxide purifier. The brief introduction of the project is as follows:
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/bootstrap-datetimepicker.min_css?action=raw&ctype=text/css" type="text/css" />
+
Our team consists of 27 students from different majors of Anhui University of Technology, including biology, mechanical engineering, art design, English, etc.<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/nice-select_css?action=raw&ctype=text/css" type="text/css" />
+
We were divided into groups of students in different majors, including those responsible for experiment, art design, web programming, social practice and cooperation, and mathematical modeling.<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/owl.carousel.min_css?action=raw&ctype=text/css" type="text/css" />
+
At the same time, as we are from a country where the mother tongue is not English, we have arranged some students to be responsible for English translation and English presentation.<br/><br/>
−
        <!-- main css -->
+
The students in charge of the experiment are Pan Luyao, Liu Zhihao, Bian Weixin, Wang Xiangxuan, Hu Zhiyuan, Qin Zichen, Wang Huichao,Chen Yuru,Chen Jinrui.<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/style_css?action=raw&ctype=text/css" type="text/css" />
+
They completed the experimental portion of the project, building Biobricks that met  the standards of iGEM.<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/responsive_css?action=raw&ctype=text/css" type="text/css" />
+
The students responsible for art design are Sun Yu, Wang Yalong and Jiang Yu.<br/><br/>
−
        <link rel="stylesheet" href="https://2018.igem.org/Team:AHUT_China/index_css?action=raw&ctype=text/css" type="text/css">
+
They completed the design of poster, team flag, team banner, and team uniform, and they participated in the production of some pictures of wiki.<br/><br/>
−
     </head>
+
The students responsible for web programming students are Cao Jinhui, Zhu Dazhu, Tao Wei.They did all the work of edit for the wiki.
−
      
+
−
        <!--================Header Area =================-->
+
−
        <header class="header_area">
+
−
            <div class="container">
+
−
                <nav class="navbar navbar-expand-lg navbar-light">
+
−
                    <!-- Brand and toggle get grouped for better mobile display -->
+
−
                    <a class="navbar-brand logo_h" href="https://2018.igem.org/Team:AHUT_China/home"><img src="https://static.igem.org/mediawiki/2018/e/ec/T--AHUT_China--_logo.jpg" height="100" width="80" alt=""></a>
+
−
                    <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#navbarSupportedContent" aria-controls="navbarSupportedContent" aria-expanded="false" aria-label="Toggle navigation">
+
−
                        <span class="icon-bar"></span>
+
−
                        <span class="icon-bar"></span>
+
−
                        <span class="icon-bar"></span>
+
−
                    </button>
+
−
                    <!-- Collect the nav links, forms, and other content for toggling -->
+
−
                    <div class="collapse navbar-collapse offset" id="navbarSupportedContent">
+
−
                        <ul class="nav navbar-nav menu_nav ml-auto">
+
−
                            <li class="nav-item active"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/home">Home</a></li>
+
−
                            <li class="nav-item submenu dropdown">
+
−
                                <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">TEAM</a>
+
−
                                <ul class="dropdown-menu">
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Team
+
−
">Team Members</a></li>
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Attributions">Attribution</a>
+
−
<li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Sponsoring">Sponsoring</a></li>
+
−
                                </ul>
+
−
                            </li>
+
−
                            <li class="nav-item submenu dropdown">
+
−
                                <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PROJECT</a>
+
−
                                <ul class="dropdown-menu">
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Overview">Overview</a></li>
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Background">Background</a></li>
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Demonstrate">Demonstrate</a></li>
+
−
<li class="nav-item"><a class="nav-link" href="
+
−
https://2018.igem.org/Team:AHUT_China/InterLab">Our lab</a></li>
+
−
                                  </li>
+
−
                                </ul>
+
−
                            </li>
+
−
                              <li class="nav-item submenu dropdown">
+
−
                                <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">PARTS</a>
+
−
                                <ul class="dropdown-menu">
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Improve">Improved parts</a></li>
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/New_Parts">New Parts</a></li>
+
−
+
−
                                </ul>
+
−
                            </li>
+
−
<li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Model">Modeling</a></li>
+
−
                            <li class="nav-item submenu dropdown">
+
−
                                <a href="#" class="nav-link dropdown-toggle" data-toggle="dropdown" role="button" aria-haspopup="true" aria-expanded="false">Human Practices</a>
+
−
                                <ul class="dropdown-menu">
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Human_Practices">HP</a></li>
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/HP_FOR_SILVER">HP for Silver</a></li>
+
−
                                    <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/INTEGRATED_FOR_GOLD">Integrated HP for gold</a></li>
+
−
                                </ul>
+
−
                            </li>
+
−
                            <li class="nav-item"><a class="nav-link" href="https://2018.igem.org/Team:AHUT_China/Collaborations">Collaboration</a></li>
+
−
<!--                            <li class="nav-item"><a class="nav-link" href="contact.html">Contact</a></li>-->
+
−
                        </ul>
+
−
                    </div>
+
−
                </nav>
+
−
             </div>
+
−
         
+
−
        </header>
+
−
        <!--================Header Area =================-->
+
−
        <div class="section4">
+
−
<div class="sec4-right">
+
−
<div class="sec4-img-2"></div>
+
−
<div class="sec4-img-3"></div>
+
−
<div class="sec4-img-9"></div>
+
−
<div class="sec4-img-4"></div>
+
−
<div class="sec4-img-10"></div>
+
−
<div class="sec4-img-5"></div>
+
−
<div class="sec4-img-11"></div>
+
−
<div class="sec4-img-6"></div>
+
−
<div class="sec4-img-7"></div>
+
−
<div class="sec4-img-8"></div>
+
−
<div class="sec4-img-1"></div>
+
−
</div>
+
−
</div>
+
−
        <!--================Breadcrumb Area =================-->
+
−
       
+
−
        <!--================Breadcrumb Area =================-->
+
−
        <!--================ Latest Blog Area  =================-->
+
−
        <section class="latest_blog_area section_gap">
+
−
            <div class="container">
+
−
              <div class="section_title ">
+
−
                <div align="center"> <h2 class="title_color">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Introduction</h2></div>
+
−
                  <p>We took part in the Fifth International InterLab Measurement Study which ains to achieve the purpose of comparative measurement. The goal of this study is to obtain large amounts of data from labs across the world,to develop absolute units for measurements GFP in a plate reader to eliminate variation between labs.</p><br>
+
−
<div align="center"><h2 class="title_color">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Materials</h2></div>
+
−
                  <p>Plate reader: Synergy H1 (Biotek)<br>
+
−
Plate reader plates: Corning 3603 96-Well Microplates (black plates with clear flat bottom)<br>
+
−
Cell culture shaker: ZWYR-200D<br><br>
+
−
Devices:<br>
+
−
Negative control ：BBa_R0040 <br>
+
−
Positive control ：BBa_I20270 <br>
+
−
Device 1： BBa_J364000  <br>
+
−
Device 2： BBa_J364001  <br>
+
−
Device 3： BBa_J364002  <br>
+
−
Device 4： BBa_J364007  <br>
+
−
Device 5： BBa_J364008  <br>
+
−
Device 6： BBa_J364009  <br>
+
−
Note: for Device 5, we have not transformed it into DH5⍺ competent cells successfully for many times, therefore, we thank IGEM team of Nanjing University for providing the Device 5.<br>
+
−
Calibration material: Provided in the 2018 IGEM distribution kit <br>
+
−
Microorganism: Escherichia coli DH5⍺ strains<br>
+
−
</p><br>
+
−
<div align="center"><h2 class="title_color">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Methods</h2></div>
+
−
                  <p>Following iGEM requirements, Team AHUT_China performed measurements according to these 2018 InterLab Protocols <a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf">https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf</a> </p><br>
+
−
<div align="center"><h2 class="title_color">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Results</h2></div>
+
−
                  <h4>1.OD 600 reference point</h4><p>
+
−
Using OD 600 and H2O to generate the conversion factor for the transformation later. The average of OD600 is 0.063; the correction factor (OD600/ABS600) is 3.500
+
−
</p><br>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/c/cb/T--AHUT_China--_LUDOX_correct_result.jpg" width="317" height="234" alt=""/></div><br><div align="center">Fig. 1 LUDOX correct value
+
−
  </div>
+
−
  <h4>2.Particle standard curve</h4>
+
−
                  <p>
+
−
We obtained the two Particle Standard Curve (normal and log scale).
+
−
</p><br>
+
−
  <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/65/T--AHUT_China--_Fig._2_Particle_Standard_Curve.jpg" width="701" height="440" alt=""/></div><br><div align="center">Fig. 2 Particle Standard Curve
+
−
  </div>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/7/7e/T--AHUT_China--_Fig._3_Particle_Standard_Curve_%28log_scale%29.jpg" width="701" height="440" alt=""/></div><br><div align="center">
+
−
  Fig. 3 Particle Standard Curve (log scale)
+
−
</div>
+
−
<h4>3.Fluorescein standard curve</h4><p>
+
−
Dilution serious of fluorescein were prepared and measured in a 96 well plate. A standard curve is generated to correct the cell based readings to an equivalent fluorescein concentration.<br>
+
−
We obtained the two Fluorescein Standard Curve (normal and log scale).
+
−
</p><br>
+
−
 
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/24/T--AHUT_China--_Fig._4_Fluorescein_Standard_Curve.jpg" width="701" height="440" alt=""/></div><br><div align="center">
+
−
  Fig. 4 Fluorescein Standard Curve
+
−
</div>
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/a/a9/T--AHUT_China--_Fig._5_Fluorescein_Standard_Curve_%28log_scale%29.jpg" width="701" height="440" alt=""/></div><br><div align="center">
+
−
  <div align="center" >Fig. 5 Fluorescein Standard Curve (log scale) </div>
+
−
</div>
+
−
+
−
  <h4>4.Cell measurements</h4>
+
−
  </ol>
+
−
<p>&nbsp;</p><br>
+
−
 
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/21/T--AHUT_China--_Fig._6_Fluorescence_Measurements_Curve_.jpg" width="732" height="492" alt=""/></div><br><div align="center">
+
−
  <div align="center">Fig. 6 Fluorescence Measurements Curve</div>
+
−
</div>
+
−
    <p>Test devices 1 and 4 show high fluorescence intensity. Test devices 2 show a modest fluorescence intensity alone with positive control group, while devices3,5,6 barely show low fluorescence intensity alone with the negative control group.
+
−
</p><br>
+
−
 
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/3/36/T--AHUT_China--_Fig._7_Raw_OD600_Curve_.jpg" width="724" height="484" alt=""/></div><br><div align="center">
+
−
  <div align="center">Fig. 7 Raw OD600 Curve</div>
+
−
</div>
+
−
    <h4>5.We obtained the Colony Forming Units per 0.1 OD600 E. coli cultures</h4>  
+
−
<div align="center"><img src="https://static.igem.org/mediawiki/2018/f/f1/T--AHUT_China--_Fig._8_CFU_Result.jpg" width="724" height="420" alt=""/></div><br>
+
−
    <div align="center"><img src="https://static.igem.org/mediawiki/2018/e/e5/T--AHUT_China--_Fig._8_CFU_Result1.jpg" width="732" height="492" alt=""/></div><br>
+
−
    <div align="center">
+
−
  <div align="center" >Fig. 8 CFU Result</div>
+
−
  <p >&nbsp;</p>
+
−
<div align="center"><h2 class="title_color">Discussion</h2></div>
+
−
                <p>For Figure 3, the log graph isn’t a straight line but not 1:1 slope. In figure 6, highest fluorescence was obtained from device 4, closely followed by test device 1. Test device 2 and positive control group show a modest fluorescence intensity and device 5,6 show low fluorescence intensity, while test devices 3 barely have any fluorescence signal as well as the negative group.  
+
−
</p>            
+
−
<div align="center"><h2 class="title_color">Conclusion</h2></div>
+
−
                <p>It was certainly a technical challenge to Participate in the InterLab Study. Performing the prescribed protocols with adherence to all the InterLab guidelines yielded parts of expected results, and with the completed InterLab Google Forms, confirms our team participation in this InterLab Study.
+
−
</p>
+
−
               
+
−
                 
+
−
            </div>
+
−
          </div>
+
−
            </div>
+
−
        </section>
+
−
        <!--================ Recent Area  =================-->
+
−
       
+
−
     
+
−
       
+
−
        <!--================ start footer Area  =================-->
+
−
        <DIV style="background: rgba(1,1,1,1.00)"><div align="center"><video height="200px" width="800px" controls="controls"><source src="https://static.igem.org/mediawiki/2018/9/91/T--AHUT_China--_foot.mp4" type="video/mp4" ></div>
+
−
                  <br></div>
+
−
     
+
  
−
        <!-- Modals error -->
 
  
−
     
 
−
        <!--================End Contact Success and Error message Area =================-->
 
−
       
 
−
       
 
−
        <!-- Optional JavaScript -->
 
−
        <!-- jQuery first, then Popper.js, then Bootstrap JS -->
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/jquery-3.2.1.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/popper_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/bootstrap.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/owl.carousel.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/jquery.ajaxchimp.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/bootstrap-datetimepicker.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/jquery.nice-select.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/mail-script_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/stellar_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/imagesloaded.pkgd.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/isotope-min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/stellar_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/simpleLightbox.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
        <!--gmaps Js-->
 
−
        <script src="https://maps.googleapis.com/maps/api/js?key=AIzaSyCjCGmQ0Uq4exrzdcL6rvxywDDOvfAu6eE?action=raw&ctype=text/css" type="text/css""></script>
 
−
        <script src="https://2018.igem.org/Team:AHUT_China/gmaps.min_js?action=raw&ctype=text/css" type="text/css""></script>
 
−
        <!-- contact js -->
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/jquery.form_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/jquery.validate.min_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/contact_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/custom_js?action=raw&ctype=text/javascript" type="text/javascript" ></script>
 
−
      <script src="https://2018.igem.org/Team:AHUT_China/move_js?action=raw&ctype=text/javascript" type="text/javascript"></script>
 
  
−
<script>
+
 
−
setInterval(function () {
+
        }}
−
move('.sec4-img-1').set('bottom', '300px').set('opacity', '0').duration('2s').ease('in-out').end(function () {
+
    }}
−
move('.sec4-img-1').set('bottom', '561px').set('opacity', '1').duration('0.1s').end();
+
}}
−
});
+
{{Heidelberg/footer}}
−
move('.sec4-img-2').rotate(360).duration('15s').end(function () {
+
−
move('.sec4-img-2').rotate(0).duration('0.01s').end();
+
−
});
+
−
move('.sec4-img-3').rotate(-360).duration('15s').end(function () {
+
−
move('.sec4-img-3').rotate(0).duration('0.01s').end();
+
−
});
+
−
move('.sec4-img-4').rotate(360).duration('15s').end(function () {
+
−
move('.sec4-img-4').rotate(0).duration('0.01s').end();
+
−
});
+
−
move('.sec4-img-5').rotate(-360).duration('15s').end(function () {
+
−
move('.sec4-img-5').rotate(0).duration('0.01s').end();
+
−
});
+
−
move('.sec4-img-6').rotate(360).duration('15s').end(function () {
+
−
move('.sec4-img-6').rotate(0).duration('0.01s').end();
+
−
});
+
−
move('.sec4-img-7').rotate(-360).duration('15s').end(function () {
+
−
move('.sec4-img-7').rotate(0).duration('0.01s').end();
+
−
});
+
−
}, 500);
+
−
</script>
+
 
</html>
 
</html>

Revision as of 04:01, 7 October 2018

Royal Hotel Template:Heidelberg/main Template:Heidelberg/footer </html>