Difference between revisions of "Team:SKLMT-China/Material"

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<h2data-content="SKLMT-China"><span>SKLMT-China</span></h2>
 
</div>
 
</section>
 
</header>
 
<p>This is <strong>SKLMT-China</strong> test word
 
<br/>
 
iGEM team from sdu test banner
 
<br/>
 
            wiki Here try long sentences
 
</div>
 
</section>
 
 
 
<divclass="container">
 
 
<divclass="paragraph">
 
<h2>Description</h2>
 
<p>QIAquick Gel Extraction Kit (Qiagen, cat. no. 28704)
 
QIAquick PCR Purification Kit (Qiagen, cat. no. 28104)
 
Qiagen Plasmid Mini Kit (Qiagen, cat. no. 12123)
 
Tryptone (Oxoid, cat. no. LP0042)
 
Yeast extract (Oxoid, cat. no. LP0021)
 
NaCl (Sangon Biotec, cat. no. SB0476)
 
Agar (Solarbio, cat. no. A8190)
 
NaOH (Sigma-Aldrich, cat. no. S8045)
 
Autoclaved ddH
 
2O kept at room temperature (RT, 18–22 °C) and on ice
 
Tetracycline hydrochloride (Sigma-Aldrich, cat. no. T3383)
 
Chloramphenicol (Sigma-Aldrich, cat. no. C1919)
 
Ampicillin sodium salt (Sigma-Aldrich, cat. no. A9518)
 
Kanamycin sulfate (Sigma-Aldrich, cat. no. K4000)
 
Apramycin sulfate salt (Sigma-Aldrich, cat. no. A2024)
 
Gentamicin solution (50 mg ml − 1; Sigma-Aldrich, cat. no. G1397)
 
Hygromycin B (Invitrogen, cat. no. 10687-010)
 
Streptomycin sulfate salt (Sigma-Aldrich, cat. no. A6501)
 
Sodium acetate trihydrate (NaOAc•3H2O; Sinopharm, cat. no. 10018718)
 
Lysozyme (Sigma-Aldrich, cat. no. 62970)
 
Proteinase K (Roche, cat. no. 03115879001)
 
Trizma base (Sigma-Aldrich, cat. no. T6066)
 
Na2EDTA (Sigma-Aldrich, cat. no. E5134)
 
HCl (Sinopharm, cat. no. 10011008) Sodium dodecyl sulfate (SDS; Sigma-Aldrich, cat. no. L3771)
 
Phenol–chloroform–isoamyl alcohol mixture (25:24:1; Sigma-Aldrich,cat. no. 77617)
 
Isopropanol (Sinopharm, cat. no. 80109218)
 
Ethanol, absolute (Sinopharm, cat. no. 10009218)
 
70% (vol/vol) ethanol
 
RNase A (10 mg ml − 1; DNase and protease free; Thermo Scientific,cat. no. EN0531)
 
l-Arabinose (Sigma-Aldrich, cat. no. A3256)
 
l-Rhamnose (Sigma-Aldrich, cat. no. 83650)
 
1-kb DNA ladder (New England BioLabs, cat. no. N3232)
 
Restriction enzymes (New England BioLabs)
 
PrimeSTAR Max DNA Polymerase (Takara, cat. no. R045B)
 
10× Tris/boric acid/EDTA (TBE) buffer (Bio-Rad, cat. no. 161-070)
 
Ethidium bromide solution (10 mg/ml; Dingguo, cat. no. NEP028-1)
 
Agarose (Takara, cat. no. 5261)
 
Buffer P1 (Qiagen, cat. no. 19051)
 
Buffer P2 (Qiagen, cat. no. 19052)
 
Buffer P3 (Qiagen, cat. no. 19053)
 
</p>
 
 
</div>
 
 
</div>
 
<sectionid="page-footer">
 
<divclass="container">
 
<divclass="row">
 
<divclass="col-md-12"style="padding-top: 3em;">
 
<h2><strong>SKLMT</strong></h2>
 
</div>
 
</div>
 
<divclass="row">
 
<divclass="col-md-4">
 
<h3>Useful <strong>&thinsp;Links</strong></h3>
 
<p>
 
<ahref="https://igem.org/Main_Page">Main Page</a>
 
</p>
 
<p>
 
<ahref="https://igem.org/Special:SpecialPages">Special pages</a>
 
</p>
 
</div>
 
<divclass="col-md-4">
 
<h3><strong>Contact&thinsp;</strong> us</h3>
 
<br>
 
<iclass ="fa fa-envelope-o fa-lg">&thinsp;&thinsp;SKLMT.iGEM.China@gmail.com</i>
 
</div>
 
<divclass="col-md-4">
 
<h3><strong>Address</strong></h3>
 
<br>
 
<iclass="fa fa-map-marker fa-lg">&thinsp;&thinsp;Qingdao, Shandong<br><br><br>266200 Shandong University</i>
 
</div>
 
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Revision as of 04:30, 7 October 2018