Difference between revisions of "Team:KCL UK"

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{{KCL_UK}}
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<html>
 
<html>
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<div class="column full_size" >
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    <head>
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<h1> Welcome to the King’s College London 2018 Team Wiki! </h1>
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<link href="https://fonts.googleapis.com/css?family=Raleway" rel="stylesheet">
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<p> In our project we are developing a new technology platform based on the knowledge of the role of sRNAs in bacteria to control antibiotic resistance. We will use E.coli K12 to test our strategies by engineering libraries of sRNA scaffolds  to modulate gene expression patents in relation to the external environmental factors.</p>
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    <title>KCL iGEM</title>
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a:visited {
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}
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}
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        * {
 +
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            font-family: 'Raleway', sans-serif;
 +
        }
 +
 
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 +
        body{
 +
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 +
        font-family: 'Raleway', sans-serif;
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        img {
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 +
 
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 +
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            margin-bottom: 1em;
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            padding-bottom: 2em;
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            text-emphasis: bold;
 +
        }
 +
        .smallertitle {
 +
          font-weight: 900;
 +
          font-weight: bold;
 +
          font-size: 1.4rem;
 +
          margin-bottom: 1em;
 +
          padding-top: 3.1em;
 +
          padding-bottom: 0em;
 +
          text-emphasis: bold;
 +
 
 +
        }
 +
        h1 {
 +
            font-weight: 300;
 +
            font-size: 2.5em;
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            text-transform: capitalize;
 +
            margin-top: 1em;
 +
          }
 +
        .title span {
 +
            margin-top: 1em;
 +
            font-weight: 200;
 +
            display: block;
 +
            font-size: .9em;
 +
        }
 +
 
 +
 
 +
        /*buttonmasks -------------------------------*/
 +
        .buttonmask {
 +
            display: inline-block;
 +
            font-size: 1.25rem;
 +
            text-decoration: none;
 +
            text-transform: uppercase;
 +
            border-width: 2px;
 +
            border-style: solid;
 +
            padding: .5em 1.75em;
 +
 
 +
        }
 +
  .buttonmask}
 +
 
 +
        .buttonmask-small {
 +
            font-size: .9rem;
 +
            font-weight: 700;
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        .buttonmask-accent {
 +
            color: #FF0000;
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            border-color: #FF0000;
 +
        }
 +
 
 +
        .buttonmask-accent:hover {
 +
        background: #FF0000;
 +
        color: black;
 +
        }
 +
 
 +
 
 +
        /*header------------------------------*/
 +
 
 +
        header {
 +
            background-color: rgba(0,0,0,.75);
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 +
            right: 0;
 +
            max-height: 7em;
 +
        }
 +
 
 +
        nav ul {
 +
            margin: 0;
 +
            padding: 0;
 +
            list-style: none;
 +
            float: right;
 +
            margin-right: 5em!important;
 +
            margin-top: 2em;
 +
            position: relative;
 +
        }
 +
 
 +
        nav li {
 +
            display: inline-block;
 +
            margin: 1em;
 +
            position: relative;
 +
        }
 +
 
 +
        .nav-buttonmasks {
 +
            font-weight: 900;
 +
            text-decoration: none;
 +
            text-transform: uppercase;
 +
            font-size: 0.95rem;
 +
            padding: .5em;
 +
            color: #FFF;
 +
            position: relative;
 +
            padding-bottom: 3em;
 +
        }
 +
 
 +
        .nav-buttonmasks:hover {color: #FF0000;}
 +
        .dropdown-nav-buttonmasks:hover {color: #FF0000;}
 +
 
 +
        .dropdown-nav-buttonmasks {
 +
            font-weight: 900;
 +
            text-decoration: none;
 +
            text-transform: uppercase;
 +
            font-size: 0.95rem;
 +
            padding: .5em;
 +
            color: #FFF;
 +
            display: none;
 +
            background-color:  rgba(0,0,0,.75);
 +
            z-index: 1;
 +
            width: 10em;
 +
            margin-left: -3em;
 +
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 +
        #navadjust {
 +
          margin-left:-0.3em;
 +
        }
 +
 
 +
        .dropdownone {
 +
          margin-top: 2.58em!important;
 +
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 +
        .dropdowntwo {
 +
            margin-top: 4.75em!important;
 +
        }
 +
        .dropdownthree {
 +
          margin-top: 6.93em!important;
 +
        }
 +
 
 +
        .nav-buttonmasks:hover .dropdown-nav-buttonmasks{
 +
            display: block;
 +
            position: absolute;
 +
        }
 +
        .logo {
 +
            height: 90px;
 +
            width: 90px;
 +
            float: left;
 +
            margin-left: 6em;
 +
            margin-top: .5em;
 +
        }
 +
 
 +
          /*home hero------------------------------*/
 +
 
 +
        .home-hero {
 +
            background-image: url(https://static.igem.org/mediawiki/2018/e/ed/T--KCL_UK--homehero.png);
 +
            background-repeat: no-repeat;
 +
            background-size: cover;
 +
            padding: 10em 0;
 +
            color: #FFF;
 +
            height: 100vh;
 +
            background-attachment: fixed;
 +
            background-position: center;
 +
        }
 +
 
 +
        #seeourwork {
 +
          margin-top: 0em;
 +
          margin-bottom: 2em;
 +
        }
 +
 
 +
  /*home-about------------------------------*/
 +
        .home-about-textbox {
 +
            background-color: black;
 +
            padding: 15em;
 +
 
 +
            margin-left: -2.5vw;
 +
            outline: 2px solid #FF0000;
 +
            outline-offset: -5em;
 +
            color: #FFF;
 +
        }
 +
 
 +
        .home-about-textbox h1 {
 +
            color: #FF0000;
 +
            position: relative;
 +
            margin-top: -5em;
 +
            margin-bottom: 3em;
 +
            background: black;
 +
            margin-left: auto;
 +
            margin-right: auto;
 +
            width: 35vw;
 +
            text-align: center;
 +
       
 +
        }
 +
        .home-about-textbox a {
 +
            margin-top: 2em;
 +
            margin-bottom: 0;
 +
            width: 60%;
 +
        }
 +
 
 +
        #projectdescshort {
 +
          color: white;
 +
font-size: 1.4em!important;
 +
margin-top: 2em!important;
 +
padding-top:2em!important;
 +
         
 +
     
 +
        }
 +
 
 +
 
 +
 
 +
      /*portfolio------------------------------*/
 +
 
 +
        .portfolio h1 {
 +
            width: 12em;
 +
            margin: auto;
 +
            margin-top: -1.5em;
 +
            padding-bottom: .7em;
 +
            padding-top: 1em;
 +
            color: #FF0000;
 +
        }
 +
        .line {
 +
                width: 30%;
 +
                height: 1px;
 +
                margin: auto;
 +
            background: #FF0000;
 +
        }
 +
        .spacer {
 +
            width: 100%;
 +
            height: 2em;
 +
        }
 +
        .portfolio {
 +
            margin: 3em 0;
 +
            background-color: black;
 +
 
 +
        }
 +
 
 +
        .port-item {
 +
            position: relative;
 +
            margin: 0;
 +
            width: 50%;
 +
            float: left;
 +
        }
 +
        .port-item img {
 +
            display: block;
 +
            width: 100%;
 +
            height: 17em;
 +
            object-fit: cover;
 +
 
 +
        }
 +
        .port-desc {
 +
            position: absolute;
 +
            z-index: 100;
 +
            bottom: 3em;
 +
            left: 3em;
 +
            right: 3em;
 +
            color: white;
 +
            background: rgba(0,0,0,.75);
 +
            padding-bottom: 1em;        }
 +
          .port-desc p {
 +
            margin: 1em;
 +
            font-size: 1.2em;
 +
 
 +
        }
 +
        #projectdescshort {
 +
          font-size: 1.5em!imporant;
 +
        }
 +
 
 +
 
 +
 
 +
 
 +
    </style>
 +
 
 +
 
 +
    </head>
 +
 
 +
 
 +
    <body>
 +
 
 +
    <header>
 +
        <img src="https://static.igem.org/mediawiki/2018/0/08/T--KCL_UK--kclfinallogo.gif" alt="logo" class="logo">
 +
 
 +
        <nav>
 +
            <ul>
 +
                <li><a href="index.html" class="nav-buttonmasks standalonenav">Home</a></li>
 +
                <li class="nav-buttonmasks">Team
 +
                  <a href="teammembers.html" class="dropdown-nav-buttonmasks dropdownone">Team Members</a>
 +
                    <a href="#" class="dropdown-nav-buttonmasks dropdowntwo">Collaborations</a>
 +
                </li>
 +
                <li class="nav-buttonmasks">Project
 +
                  <a href="#" class="dropdown-nav-buttonmasks dropdownone">Description</a>
 +
                  <a href="#" class="dropdown-nav-buttonmasks dropdowntwo">Design</a>
 +
                  <a href="#" class="dropdown-nav-buttonmasks dropdownthree">Results</a>
 +
                </li>
 +
                <li class="nav-buttonmasks">Parts
 +
                  <a href="#" class="dropdown-nav-buttonmasks dropdownone">Parts Overview</a>
 +
                  <a href="#" class="dropdown-nav-buttonmasks dropdowntwo">Basic Parts</a>
 +
                  <a href="#" class="dropdown-nav-buttonmasks dropdownthree">Composite Parts</a>
 +
                </li>
 +
                <a href="#" class="nav-buttonmasks standalonenav">Safety</a>
 +
                <li class="nav-buttonmasks">Human Practices
 +
                  <a href="integratedhumanpractices.html" class="dropdown-nav-buttonmasks dropdownone" id="navadjust">Integrated HP</a>
 +
                  <a href="educationandengagement.html" class="dropdown-nav-buttonmasks dropdowntwo" id="navadjust">Education and Engagement</a>
 +
                </li>
 +
                <a href="acknowledgements.html" class="nav-buttonmasks standalonenav">Acknowledgements</a>
 +
            </ul>
 +
        </nav>
 +
    </header>
 +
 
 +
  <div class="loading">
 +
  <div>
 +
 
  
−
<img src="https://static.igem.org/mediawiki/2018/f/f5/T--KCL_UK--kclteampic.png">
 
−
 
−
</div>
 
  
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<p>We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli. </br></br>
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We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).</p>
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            <span><h1 class="smallertitle">Investigating Untranslated regions on 3' end</h1></span>
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FULL PROJECT DESCRIPTION</a>
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            <p id="projectdescshort">We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli. <br> <br>We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).</p>
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                <p>iGEM Symposium</p>
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                <p>Bexley School Visit</p>
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<p>As a team, we strive to reach out to all communities to share knowledge which not only empowers people of all ages, but also shares what we have learned as an iGEM team doing research in synthetic biology.</p>
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<p>We document and record everything we do in the lab, so anyone can look into the research which we have been working on. In the Lab Work section you can read all about our process, testing, and model.</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
 
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
 
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
 
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
 
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Revision as of 21:50, 8 October 2018

KCL iGEM

what are we doing?

We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli.

We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).

Full Project Description

Some of Our Work

portfolio item

iGEM Symposium

See More
portfolio item

Bexley School Visit

See More
portfolio item

Social Media

See More
portfolio item

Lab Work

See More