Difference between revisions of "Team:KCL UK"
| Line 504: | Line 504: | ||
<nav> | <nav> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="https://2018.igem.org/Team:KCL_UK" class="nav-buttonmasks standalonenav">Home</a></li> |
<li class="nav-buttonmasks">Team | <li class="nav-buttonmasks">Team | ||
<a href="https://2018.igem.org/Team:KCL_UK/Team" class="dropdown-nav-buttonmasks dropdownone">Team Members</a> | <a href="https://2018.igem.org/Team:KCL_UK/Team" class="dropdown-nav-buttonmasks dropdownone">Team Members</a> | ||
Revision as of 22:16, 8 October 2018
what are we doing?
We are developing a new technology platform based on 3'UTR sequence characterisation to control soluble protein expression in E.coli.
We will use E.coli K12 to test our "Proof of Concept" Principle by engineering 3'UTR sequences (Sharma et al, 2011) to interact with either cytosolic proteins or 5'UTR sequences of our gene of interest: trxA and gor. Down regulation of these genes will be quantified by assessing quantitatively GFP signal. Efficiency of down regulation of genes will be quantified by assessing reaction kinetics of Alkaline Phosphatase (AP).
Some of Our Work
iGEM Symposium
See More
Bexley School Visit
See More
Social Media
See More
Lab Work
See More