Difference between revisions of "Team:Georgia State/sandbox"

 
(89 intermediate revisions by 2 users not shown)
Line 1: Line 1:
−
{{http://www.2017.igem.org/Template:Georgia_State_sand}}
+
{{Georgia_State/sand}}
 +
<html>
 +
<!DOCTYPE html>
 +
<!--[if lt IE 7]>      <html class="no-js lt-ie9 lt-ie8 lt-ie7"> <![endif]-->
 +
<!--[if IE 7]>        <html class="no-js lt-ie9 lt-ie8"> <![endif]-->
 +
<!--[if IE 8]>        <html class="no-js lt-ie9"> <![endif]-->
 +
<!--[if gt IE 8]><!--> <html class="no-js"> <!--<![endif]-->
 +
    <head>
 +
        <meta charset="utf-8">
 +
        <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
 +
        <title></title>
 +
        <meta name="description" content="">
 +
        <meta name="viewport" content="width=device-width">
 +
           
 +
 
 +
        <script type="text/javascript" src="https://2018.igem.org/Template:Georgia_State/JS?
 +
action=raw&ctype=text/javascript"></script>
 +
        <script type="text/javascript" src="https://2018.igem.org/Template:Georgia_State/JS/Bootstrap?
 +
action=raw&ctype=text/javascript"></script>
 +
        <script type="text/javascript" src="https://2018.igem.org/Template:Georgia_State/JS/Jquery?
 +
action=raw&ctype=text/javascript"></script>
 +
<script type="text/javascript" src="https://2018.igem.org/Template:Georgia_State/JS/Owl?
 +
action=raw&ctype=text/javascript"></script>
 +
 
 +
  <!-- Facebook and Twitter integration -->
 +
<meta property="og:title" content=""/>
 +
<meta property="og:image" content=""/>
 +
<meta property="og:url" content=""/>
 +
<meta property="og:site_name" content=""/>
 +
<meta property="og:description" content=""/>
 +
<meta name="twitter:title" content="" />
 +
<meta name="twitter:image" content="" />
 +
<meta name="twitter:url" content="" />
 +
<meta name="twitter:card" content="" />
 +
 
 +
<link href="https://fonts.googleapis.com/css?family=Raleway:100,300,400,700" rel="stylesheet">
 +
<base href="/">
 +
<!-- Animate.css -->
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Georgia_State/CSS/Animate?action=raw&ctype=text/css">
 +
     
 +
<!-- Icomoon Icon Fonts-->
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS/Icomoon?
 +
action=raw&ctype=text/css">
 +
 
 +
<!-- Themify Icons-->
 +
<link rel="stylesheet"type="text/css"  href="https://2018.igem.org/Template:Georgia_State/CSS/Themify?
 +
action=raw&ctype=text/css">
 +
 
 +
<!-- Bootstrap  -->
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS/Bootstrap?
 +
action=raw&ctype=text/css">
 +
 
 +
<!-- Magnific Popup -->
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS/Magnific?
 +
action=raw&ctype=text/css">
 +
 
 +
 
 +
<!-- Owl Carousel  -->
 +
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS/Owl?
 +
action=raw&ctype=text/css">
 +
 
 +
<!-- Theme style  -->
 +
<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Georgia_State/CSS?
 +
action=raw&ctype=text/css">
 +
     
 +
 
 +
<!-- Modernizr JS -->
 +
<script type="text/javascript" src="https://2018.igem.org/Template:Georgia_State/JS/Modernizr?
 +
action=raw&ctype=text/javascript"></script>
 +
    </head>
 +
 
 +
          <body>
 +
 +
 +
 +
<div id="page">
 +
<!-- Menu Start -->
 +
                    <div class="menu">
 +
                        <div class="navbar">
 +
                            <a class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
 +
                                <i class="fw-icon-th-list"></i>
 +
                            </a>
 +
                            <div class="nav-collapse collapse">
 +
                                    <ul class="nav">
 +
        <li><a href="https://2017.igem.org/Team:Georgia_State"  class="active">Home</a></li>
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Team
 +
                <ul class="dropdown-menu">
 +
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Team">Team</a></li>
 +
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Attributions">Attributions</a></li>
 +
                </ul>
 +
        </li>
 +
 
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Project
 +
                <ul class="dropdown-menu">
 +
                         
 +
                                <li><a href=" https://2017.igem.org/Team:Georgia_State/Description">Description</a></li>
 +
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Results">Results</a></li>                                       
 +
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Collaborations">Collaborations</a></li>
 +
                                                                                                                                                         
 +
                </ul>
 +
          </li>
 +
       
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Parts
 +
                <ul class="dropdown-menu">
 +
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li>
 +
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li>   
 +
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Contribution">Contribution</a></li>
 +
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Improve">Improvement</a></li>
 +
              </ul>
 +
          </li>
 +
        <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li>
 +
       
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Notebook
 +
                <ul class="dropdown-menu">
 +
                              <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li>
 +
                              <li><a href="https://2017.igem.org/Team:Georgia_State/Experiments">Experiments</a></li>
 +
                </ul>
 +
            </a>
 +
        </li>
 +
 
 +
        , <li class="dropdown-submenu">
 +
              <a class="dropdown-toggle" data-toggle="dropdown">Human Practices
 +
                <ul class="dropdown-menu">
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a>
 +
                              <ul><a href="https://2017.igem.org/Team:Georgia_State/HP/ASL">ASL Gallery</a></ul>
 +
                          </li>               
 +
                </ul>
 +
              </a>
 +
          </li>
 +
 
 +
          <li><a href="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</a></li>
 +
  </div>
 +
<!--/.nav-collapse -->
 +
                        </div>
 +
          <div class="mini-menu">
 +
            <label>
 +
          <select class="selectnav"onChange="window.location.href=this.value">
 +
            <option value="/Team:Georgia_State">Home</option>
 +
            <option value="/Team:Georgia_State/Team">Team</option>
 +
            <option value="/Team:Georgia_State/Attributions">Attributions</option>
 +
            <option value="/Team:Georgia_State/Results">Results</option>
 +
            <option value="/Team:Georgia_State/Collaborations">Collaborations</option>
 +
            <option value="/Team:Georgia_State/Parts">Parts</option>
 +
            <option value="/Team:Georgia_State/Description">Description</option>
 +
            <option value="/Team:Georgia_State/Design">Design</option>
 +
            <option value="/Team:Georgia_State/Contribution">Contribution</option>
 +
            <option value="/Team:Georgia_State/Improvement">Improve</option>
 +
            <option value="/Team:Georgia_State/Safety">Safety</option>
 +
            <option value="/Team:Georgia_State/Notebook">Notebook</option>
 +
            <option value="/Team:Georgia_State/Experiments">Experiments</option>
 +
            <option value="/Team:Georgia_State/Engagement">Public Engagement</option>
 +
            <option value="/Team:Georgia_State/HP/Silver">Silver</option>
 +
            <option value="/Team:Georgia_State/HP/Gold_Integrated">Gold and Integrated</option>
 +
            <option value="/Team:Georgia_State/HP/ASL">ASL Gallery</option>
 +
            <option value="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</option>
 +
          </select>
 +
          </label>
 +
          </div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
<!-- Menu End -->
 +
 
 +
 
 +
<header id="gtco-header" class="gtco-cover" role="banner">
 +
<div class="gtco-container">
 +
<div class="row">
 +
<div class="col-md-12 col-md-offset-0 text-left">
 +
<div class="display-t">
 +
<div class="display-tc">
 +
<div class="row">
 +
<div class="col-md-5 text-center header-img">
 +
<img src="https://static.igem.org/mediawiki/2018/7/78/T--Georgia_State--asl_logo.png" alt="">
 +
</div>
 +
<div class="col-md-7 copy">
 +
<h1> Georgia State University</h1>
 +
<p>Doing it #TheStateWay</p>
 +
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</header>
 +
<!-- END #gtco-header -->
 +
 
 +
 +
<div class="gtco-services gtco-section">
 +
<div class="gtco-container">
 +
<div class="row row-pb-sm">
 +
<div class="col-md-8 col-md-offset-2 gtco-heading text-center">
 +
<h2>Is Your Detector Expecting? See with HCG!</h2>
 +
<p> Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.</p>

Latest revision as of 22:38, 8 October 2018

Is Your Detector Expecting? See with HCG!

Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.