Difference between revisions of "Team:Georgia State/sandbox"

 
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                        <h1>Georgia State University</h1>
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                                <h1>A Novel Synthesis of Factor C</h1>
 
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                                <h2>A Story of Blood and Venom</h2>
 
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                          <h3>We’re Doing It #TheStateWay</h3>
 
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                          <p><br>Located at the heart of Downtown Atlanta, Georgia, GSU is among the top 20 most innovative institutions in the USA.
 
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                                <h1 class="media-heading">Mambalgin</h1>
 
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Mambalgin is a potent analgesic protein found in the venom of the Black Mamba snake (Dendroaspis polylepis). This protein has been found to inhibit acid-sensing ion channels (ASICs) in the central and peripheral nervous systems of mice through intraplantar and intrathecal injections. We are using the expression of mambalgin (the subject of last year's GSU team project) to assess our ability to produce, purify, and detect new recombinant fusion proteins with a glutathione-s-transferase tag.  </p>
 
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                                <a href="https://2017.igem.org/Team:Georgia_State/Description" class="btn">more</a>
 
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                                <h1 class="media-heading">Horseshoe Crab Factor C</h1>
 
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                                <p class="spacing-t">Horseshoe crab blood is used in a test to detect bacterial contamination in medical solutions and devices. We propose to replace this test with a synthetic protein consisting of human chorionic gonadotropin fused to factor C, the protein that activates the coagulation response in horseshoe crab blood.  Using this fusion protein and a simple pregnancy test strip we hope to create a system to detect gram negative
 
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                                bacteria without the harvesting of horseshoe crabs.
 
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                                    <h6><a href="https://www.instagram.com/igemgsu/" target="_blank">igemgsu</a></h6>
 
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                        <a class="twitter-timeline" href="https://twitter.com/GSUiGEM" data-tweet-limit="2">Tweets by GSUiGEM</a> <script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script></ul>
 
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                        <h1>Contact</h1>
 
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                            <strong>Address</strong><br>
 
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                            <span>Kell Hall Rm# 439, 24 Peachtree Center Ave SE, Atlanta, Georgia 30303</span>
 
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                          <a href="mailto:igemgsu@gmail.com" class="link"> igemgsu@gmail.com </a> /  (404)-413-5344
 
  
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                    Copyright(c) GSUiGEM / Original Design by:<a href="http://www.alltemplateneeds.com">www.alltemplateneeds.com</a>  
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<h1> Georgia State University</h1>
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<p>Doing it #TheStateWay</p>
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<h2>Is Your Detector Expecting? See with HCG!</h2>
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<p> Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.</p>

Latest revision as of 22:38, 8 October 2018

Is Your Detector Expecting? See with HCG!

Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.