Difference between revisions of "Team:Georgia State/sandbox"

 
(22 intermediate revisions by 2 users not shown)
Line 61: Line 61:
  
 
<!-- Theme style  -->
 
<!-- Theme style  -->
−
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Template:Georgia_State/CSS?
+
<link rel="stylesheet" type="text/css" href="https://2017.igem.org/Template:Georgia_State/CSS?
 
action=raw&ctype=text/css">
 
action=raw&ctype=text/css">
 
        
 
        
Line 72: Line 72:
 
           <body>
 
           <body>
 
 
−
<div class="gtco-loader"></div>
+
 
 
 
<div id="page">
 
<div id="page">
 +
<!-- Menu Start -->
 +
                    <div class="menu">
 +
                        <div class="navbar">
 +
                            <a class="btn btn-navbar" data-toggle="collapse" data-target=".nav-collapse">
 +
                                <i class="fw-icon-th-list"></i>
 +
                            </a>
 +
                            <div class="nav-collapse collapse">
 +
                                    <ul class="nav">
 +
        <li><a href="https://2017.igem.org/Team:Georgia_State"  class="active">Home</a></li>
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Team
 +
                <ul class="dropdown-menu">
 +
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Team">Team</a></li>
 +
                                    <li><a href="https://2017.igem.org/Team:Georgia_State/Attributions">Attributions</a></li>
 +
                </ul>
 +
        </li>
  
−
<nav class="gtco-nav" role="navigation">
+
        <li class="dropdown-submenu">
−
<div class="gtco-container">
+
            <a class="dropdown-toggle" data-toggle="dropdown">Project
−
+
                <ul class="dropdown-menu">
−
<div class="row">
+
                         
−
<div class="col-sm-2 col-xs-12">
+
                                <li><a href=" https://2017.igem.org/Team:Georgia_State/Description">Description</a></li>
−
<div id="gtco-logo"><a href="index.html"><img src="wiki/images/1/12/T--Georgia_State--logosmall.png" alt=""></a></div>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Results">Results</a></li>                                       
−
</div>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Collaborations">Collaborations</a></li>
−
<div class="col-xs-10 text-right menu-1">
+
                                                                                                                                                         
−
<ul>
+
                </ul>
−
<li class="active"><a href="Team:Georgia_State">Home</a></li>
+
          </li>
−
<li><a href="about.html">Team</a></li>
+
       
−
<li class="has-dropdown">
+
        <li class="dropdown-submenu">
−
<a href="services.html">Project</a>
+
            <a class="dropdown-toggle" data-toggle="dropdown">Parts
−
<ul class="dropdown">
+
                <ul class="dropdown-menu">
−
<li><a href="#">Description</a></li>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Parts">Parts</a></li>
−
<li><a href="#">Design</a></li>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Design">Design</a></li>   
−
<li><a href="#">Results</a></li>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Contribution">Contribution</a></li>
−
                                                                        <li><a href="#">Interlab</a></li>>
+
                                <li><a href="https://2017.igem.org/Team:Georgia_State/Improve">Improvement</a></li>
 +
              </ul>
 +
          </li>
 +
        <li><a href="https://2017.igem.org/Team:Georgia_State/Safety">Safety</a></li>
 +
       
 +
        <li class="dropdown-submenu">
 +
            <a class="dropdown-toggle" data-toggle="dropdown">Notebook
 +
                <ul class="dropdown-menu">
 +
                              <li><a href="https://2017.igem.org/Team:Georgia_State/Notebook">Notebook</a></li>  
 +
                              <li><a href="https://2017.igem.org/Team:Georgia_State/Experiments">Experiments</a></li>
 +
                </ul>
 +
            </a>
 +
        </li>
 +
 
 +
        , <li class="dropdown-submenu">
 +
              <a class="dropdown-toggle" data-toggle="dropdown">Human Practices
 +
                <ul class="dropdown-menu">
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/Engagement">Public Engagement</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Silver">Silver</a></li>
 +
                          <li><a href="https://2017.igem.org/Team:Georgia_State/HP/Gold_Integrated">Integrated and Gold</a>
 +
                              <ul><a href="https://2017.igem.org/Team:Georgia_State/HP/ASL">ASL Gallery</a></ul>
 +
                          </li>              
 +
                </ul>
 +
              </a>
 +
          </li>
 +
 
 +
          <li><a href="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</a></li>
 +
  </div>
 +
<!--/.nav-collapse -->
 +
                        </div>
 +
          <div class="mini-menu">
 +
            <label>
 +
          <select class="selectnav"onChange="window.location.href=this.value">
 +
            <option value="/Team:Georgia_State">Home</option>
 +
            <option value="/Team:Georgia_State/Team">Team</option>
 +
            <option value="/Team:Georgia_State/Attributions">Attributions</option>
 +
            <option value="/Team:Georgia_State/Results">Results</option>
 +
            <option value="/Team:Georgia_State/Collaborations">Collaborations</option>
 +
            <option value="/Team:Georgia_State/Parts">Parts</option>
 +
            <option value="/Team:Georgia_State/Description">Description</option>
 +
            <option value="/Team:Georgia_State/Design">Design</option>
 +
            <option value="/Team:Georgia_State/Contribution">Contribution</option>
 +
            <option value="/Team:Georgia_State/Improvement">Improve</option>
 +
            <option value="/Team:Georgia_State/Safety">Safety</option>
 +
            <option value="/Team:Georgia_State/Notebook">Notebook</option>
 +
            <option value="/Team:Georgia_State/Experiments">Experiments</option>
 +
            <option value="/Team:Georgia_State/Engagement">Public Engagement</option>
 +
            <option value="/Team:Georgia_State/HP/Silver">Silver</option>
 +
            <option value="/Team:Georgia_State/HP/Gold_Integrated">Gold and Integrated</option>
 +
            <option value="/Team:Georgia_State/HP/ASL">ASL Gallery</option>
 +
            <option value="https://igem.org/2017_Judging_Form?team=Georgia_State">Judging</option>
 +
          </select>
 +
          </label>
 +
          </div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
<!-- Menu End -->
  
−
</ul>
 
−
</li>
 
−
<li class="has-dropdown">
 
−
<a href="#">Dropdown</a>
 
−
<ul class="dropdown">
 
−
<li><a href="#">HTML5</a></li>
 
−
<li><a href="#">CSS3</a></li>
 
−
<li><a href="#">Sass</a></li>
 
−
<li><a href="#">jQuery</a></li>
 
−
</ul>
 
−
</li>
 
−
<li><a href="portfolio.html">Portfolio</a></li>
 
−
<li><a href="contact.html">Contact</a></li>
 
−
</ul>
 
−
</div>
 
−
</div>
 
−
 
−
</div>
 
−
</nav>
 
  
 
<header id="gtco-header" class="gtco-cover" role="banner">
 
<header id="gtco-header" class="gtco-cover" role="banner">
Line 144: Line 198:
 
<div class="row row-pb-sm">
 
<div class="row row-pb-sm">
 
<div class="col-md-8 col-md-offset-2 gtco-heading text-center">
 
<div class="col-md-8 col-md-offset-2 gtco-heading text-center">
−
<h2>Project Description</h2>
+
<h2>Is Your Detector Expecting? See with HCG!</h2>
−
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus placerat enim et urna sagittis, rhoncus euismod erat tincidunt. Donec tincidunt volutpat erat.</p>
+
<p> Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.</p>
−
<p><a href="https://www.youtube.com/watch?v=ntA4nKMk93Q" class="btn btn-special popup-vimeo">Watch The Video</a></p>
+
−
</div>
+
−
</div>
+
−
<div class="row row-pb-md">
+
−
<div class="col-md-4 col-sm-4 service-wrap">
+
−
<div class="service">
+
−
<h3><i class="ti-pie-chart"></i> Marketing</h3>
+
−
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Consectetur voluptatibus nesciunt, est alias deleniti ipsum fuga ullam maxime repudiandae neque, ad. Maiores quis atque, culpa rem inventore vero amet praesentium, quam sint, magnam reprehenderit doloremque.</p>
+
−
</div>
+
−
</div>
+
−
<div class="col-md-4 col-sm-4 service-wrap">
+
−
<div class="service animate-change">
+
−
<h3><i class="ti-ruler-pencil"></i> Design</h3>
+
−
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Minima quidem quae, assumenda dolores ad pariatur! Deleniti debitis, voluptatem! Omnis enim magnam perspiciatis, natus. Ipsum distinctio, voluptatibus vero laboriosam sequi! Officia fuga quam eveniet quo similique!</p>
+
−
</div>
+
−
</div>
+
−
<div class="col-md-4 col-sm-4 service-wrap">
+
−
<div class="service">
+
−
<h3><i class="ti-settings"></i> Development</h3>
+
−
<p>Lorem ipsum dolor sit amet, consectetur adipisicing elit. Dicta aperiam, maiores officia alias accusantium fugiat, doloremque voluptas, voluptatem dolore vel animi praesentium saepe unde consequuntur, eum asperiores odit aliquam error nulla impedit repellendus. Nesciunt, delectus.</p>
+
−
</div>
+
−
</div>
+
−
 
+
−
<div class="clearfix visible-md-block visible-sm-block"></div>
+
−
 
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<!-- END .gtco-services -->
+
−
 
+
−
+
−
+
−
 
+
−
<footer id="gtco-footer" class="gtco-section" role="contentinfo">
+
−
<div class="gtco-container">
+
−
<div class="row row-pb-md">
+
−
<div class="col-md-4 gtco-widget gtco-footer-paragraph">
+
−
<h3>Contact Us</h3>
+
−
<p>Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus placerat enim et urna sagittis, rhoncus euismod.</p>
+
−
</div>
+
−
<div class="col-md-4 gtco-footer-link">
+
−
<div class="row">
+
−
<div class="col-md-6">
+
−
<ul class="gtco-list-link">
+
−
<li><a href="#">Home</a></li>
+
−
<li><a href="#">Features</a></li>
+
−
<li><a href="#">Products</a></li>
+
−
<li><a href="#">Testimonial</a></li>
+
−
<li><a href="#">Contact</a></li>
+
−
</ul>
+
−
</div>
+
−
<div class="col-md-6">
+
−
<p>
+
−
<a href="tel://1234567890">+1 234 4565 2342</a> <br>
+
−
<a href="#">info@domain.com</a>
+
−
</p>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<div class="col-md-4 gtco-footer-subscribe">
+
−
<a class="twitter-timeline" href="https://twitter.com/GSUiGEM" data-tweet-limit="2">Tweets by GSUiGEM</a> <script async src="//platform.twitter.com/widgets.js" charset="utf-8"></script></ul>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
<div class="gtco-copyright">
+
−
<div class="gtco-container">
+
−
<div class="row">
+
−
<div class="col-md-6 text-left">
+
−
<p><small>&copy; 2016 Free HTML5. All Rights Reserved. </small></p>
+
−
</div>
+
−
<div class="col-md-6 text-right">
+
−
<p><small>Designed by <a href="http://gettemplates.co/" target="_blank">GetTemplates.co</a> Demo Images: <a href="http://pixeden.com/" target="_blank">Pixeden</a> &amp; <a href="http://unsplash.com" target="_blank">Unsplash</a></small> </p>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
</footer>
+
−
 
+
−
</div>
+
−
 
+
−
<div class="gototop js-top">
+
−
<a href="#" class="js-gotop"><i class="icon-arrow-up"></i></a>
+
−
</div>
+
−
           
+
−
          </body>
+
−
</html>
+

Latest revision as of 22:38, 8 October 2018

Is Your Detector Expecting? See with HCG!

Detection is used in all aspects of biology and is essential in providing an illustration of the chemical world around us. Currently, fluorescent protein are used as reporters but they require additional analysis with expensive and immobile equipment. We propose to create an alternative detection system kit using recombinant Human Chorionic Gonadotropin (HCG) as a reporter. The goal of our project is to create an easy, cost-effective, and sensitive detection device for use in synthetic biology, it can even be used by other iGEM teams to get an all-or-nothing response to indicate the presence or absence of targeted protein. We plan to create a pGEX plasmid containing the recombinant beta subunit of HCG with a strong promoter as a positive promoter and an additional plasmid containing recombinant HCG preceded by restriction sites not present elsewhere in the plasmid and this will be where the promoter is inserted. The promoter will only synthesize HCG when it is activated by the presence of the protein in question. Then when a pregnancy test strip is inserted in the sample, it will trigger the response based on the activation of the introduced promoter.