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| | <div id="welcome"> | | <div id="welcome"> |
| | <div id="overlay"></div> | | <div id="overlay"></div> |
| − | <p id="titre-image">CONSERVATION</p>
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| − | </div>
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| − | <div id="sous-menu">
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| − | <ul>
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| − | <li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes">HOME</a>
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| − | </li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/project" >PROJECT </a>
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| − | <ul>
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| − | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/biology">BIOLOGY</a><ul><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/selection" id="current-menu">SELECTION</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/phage_lysis">PHAGE LYSIS AND EXTRACTION</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/construction">CONSTRUCTION</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/hybridation">HYBRIDATION</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/conservation">CONSERVATION</a>
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| − | </li></ul></li>
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| − | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/engineering" >ENGINEERING</a><ul><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/pipetting_module">PIPETTING MODULE</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/fluorescence_module">FLUORESCENCE MODULE</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/temperature_module">TEMPERATURE MODULE</a>
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| − | </li><li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/purification_module">PURIFICATION MODULE</a>
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| − | </li></ul></li>
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| − | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/proof_of_concept">PROOF OF CONCEPT</a></li>
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| − | </ul>
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| − | </li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/modeling">
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| − | MODELING
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| − | </a>
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| − | </li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/human_practices">HUMAN PRACTICES</a>
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| − | </li>
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| − | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/safety">SAFETY & SECURITY</a>
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| − | </li>
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| − | <li>
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/ressources">RESSOURCES</a>
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| − | </li>
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| − | </ul>
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| − | </div>
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| | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> | | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> |
| | Our Improved Part</h3> | | Our Improved Part</h3> |
| − | Our improved part, beta-lactamase_E102F, was designed to confirm the generative capabilities of our software package. Our algorithm has proven to be successful by engineering the enzyme to exhibit more than twice the activity of the wildtype protein. | + | |
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| | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> | | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> |
| | Our Part List</h3> | | Our Part List</h3> |
| − | Here, we present the complete list of all parts that compose our evolutionary toolbox. Thereby, we aim to provide the synthetic biology community with all essential parts for their own accelerated evolution.
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| | </a> | | </a> |
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| | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> | | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> |
| | Our Parts Collection</h3> | | Our Parts Collection</h3> |
| − | We present the first self-contained in vivo evolution toolbox comprising 26 standardized BioBricks. At the heart of our toolbox is an innovative workflow for enzyme engineering, paving the way towards bio-production of novel compounds. It comprises promiscuous enzyme candidates (CYP1A2 and Cytochrome c) and selection-mediating accessory parts such as our riboswitch for detection of an organosilicon (BBa_K2398555).
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| | </a> | | </a> |
| | </div> | | </div> |
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| | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> | | <h3 style="line-height: 50px !important; margin-top:0px; text-align:left"> |
| | Our RFC</h3> | | Our RFC</h3> |
| − | Our BBF RFC combines the methodical aspects of this year´s iGEM Team Heidelberg project, presenting a new golden-gate cloning standard for simple production of geneIII-deficient phages carrying any gene-of-interest and the first accessory plasmid construction kit for selection of the best phage library member on the one hand, while having our easy-to-use and low-cost bacteriophage based in vivo directed method PREDCEL (Phage-RElated DisContinous EvoLution) on the other hand. Together with our ODE-based modeling and computer simulations performed in context of AIGEM, our RFC opens up the principle of Phage-assisted continous evolution (PACE) to the scientific world, enabling every other iGEM team to perform directed evolution on their own without the highly challenging task of setting up a PACE apparatus.
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| | </a> | | </a> |
| | </div> | | </div> |
