Difference between revisions of "Team:SBS SH 112144/InterLab"
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<h1>1. Interlab Study</h1> | <h1>1. Interlab Study</h1> | ||
<p> As a first step to contribute to this year's iGEM competition, we decided to participate in iGEM's fifth International Interlaboratory Study along with many other teams around the world. This study is organized by iGEM's measurement committee in an effort to establish standardized, reliable and repeatable measurement tools for the iGEM community and the synthetic biology community as a whole. This year's iGEM InterLab study is about establishing a standardized protocol for the measurement of GFP using a plate reader. To start things off, we needed a plate reader, Tecan Infinite M200 Pro plate reader, which is qualified to measure GFP fluorescence. Additionally, we needed competent E.coli cells DH5α. These were prepared from glycerol stocks. Together with the material iGEM had provided we were ready for work. Throughout our experiments, we tested 8 plasmids (2 controls and 6 test devices) by measuring the OD600 and the fluorescence of the cells carrying the plasmids. Besides, we calculated the Colony Forming Units per 0.1 OD600 E. coli cultures. The workflow can be separated into six segments. </p> | <p> As a first step to contribute to this year's iGEM competition, we decided to participate in iGEM's fifth International Interlaboratory Study along with many other teams around the world. This study is organized by iGEM's measurement committee in an effort to establish standardized, reliable and repeatable measurement tools for the iGEM community and the synthetic biology community as a whole. This year's iGEM InterLab study is about establishing a standardized protocol for the measurement of GFP using a plate reader. To start things off, we needed a plate reader, Tecan Infinite M200 Pro plate reader, which is qualified to measure GFP fluorescence. Additionally, we needed competent E.coli cells DH5α. These were prepared from glycerol stocks. Together with the material iGEM had provided we were ready for work. Throughout our experiments, we tested 8 plasmids (2 controls and 6 test devices) by measuring the OD600 and the fluorescence of the cells carrying the plasmids. Besides, we calculated the Colony Forming Units per 0.1 OD600 E. coli cultures. The workflow can be separated into six segments. </p> | ||
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| + | <h3>1.1 Transformation of 8 test devices into competent DH5α cells</h3> | ||
<img src="https://2018.igem.org/File:T--SBS_SH_112144--111.png"> | <img src="https://2018.igem.org/File:T--SBS_SH_112144--111.png"> | ||
Revision as of 03:50, 10 October 2018
1. Interlab Study
As a first step to contribute to this year's iGEM competition, we decided to participate in iGEM's fifth International Interlaboratory Study along with many other teams around the world. This study is organized by iGEM's measurement committee in an effort to establish standardized, reliable and repeatable measurement tools for the iGEM community and the synthetic biology community as a whole. This year's iGEM InterLab study is about establishing a standardized protocol for the measurement of GFP using a plate reader. To start things off, we needed a plate reader, Tecan Infinite M200 Pro plate reader, which is qualified to measure GFP fluorescence. Additionally, we needed competent E.coli cells DH5α. These were prepared from glycerol stocks. Together with the material iGEM had provided we were ready for work. Throughout our experiments, we tested 8 plasmids (2 controls and 6 test devices) by measuring the OD600 and the fluorescence of the cells carrying the plasmids. Besides, we calculated the Colony Forming Units per 0.1 OD600 E. coli cultures. The workflow can be separated into six segments.
1.1 Transformation of 8 test devices into competent DH5α cells
InterLab
Bronze Medal Criterion #4
Standard Tracks: Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
For teams participating in the InterLab study, all work must be shown on this page.