Difference between revisions of "Team:AHUT China/Notebook"
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<p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;"> <span style="font-size: 24px"> Western</span> blot identification of proteins<br> CA2L203K CA2WT</p> <br> | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;"> <span style="font-size: 24px"> Western</span> blot identification of proteins<br> CA2L203K CA2WT</p> <br> | ||
<div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="971" height="167" alt=""/></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/60/T--AHUT_China--93.1.png" width="971" height="167" alt=""/></div> | ||
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| + | <div class="section_title "> | ||
| + | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-style: normal; font-weight: 400; font-size: 36px; text-align: center;"> <strong style="font-family: Segoe, 'Segoe UI', 'DejaVu Sans', 'Trebuchet MS', Verdana, sans-serif; font-style: normal; font-weight: 400;color: #000000;">9.04</strong></h2> | ||
| + | <hr> | ||
| + | <nbsp><nbsp> | ||
| + | <p style="font-family: 'Arial Unicode MS', 'Microsoft YaHei UI', 'Microsoft YaHei UI Light', '华文细黑', '微软雅黑', '幼圆'; font-size: 16px;"> <span style="font-size: 24px"> Quantification</span> of protein concentrations</p> | ||
| + | <br> | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/6/6e/T--AHUT_China--93.2.png" width="908" height="189" alt=""/></div> | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/8/8b/T--AHUT_China--93.3.png" width="754" height="343" alt=""/></div> | ||
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Revision as of 11:42, 10 October 2018
8.29
Pick Clon
8.30
Pilot Expression of Protein
“IPTG+ 4h”“IPTG- 4h”
After the rocker culture is 4 H, add IPTG(final concentration is 0.5 mm) and then the rocker culture is 4 hours. Each of the two tubes absorbs 200 μL of bacterial fluid, and in the frozen centrifuge, it is 4 °C and 12000 rpm centrifuge. 10 minutes, go to the clear, It is resuspended with 100 μL 1x SDS Loading buffer, then heated in a water bath pot at 100 °C for 10 minutes and stored in a -20 °C refrigerator.
8.31
Protein extraction
CA2L203K
CA2WT
CA2L203K
9.01
Purification of Protein
Prior to purification
Purified
9.03
Western blot identification of proteins
CA2L203K CA2WT
9.04
Quantification of protein concentrations
