Difference between revisions of "Team:TPHS San Diego/Description"

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        <title>Project Description</title>
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<h1>Description</h1>
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    background: url(http://www.slopemedia.org/wp-content/uploads/2016/09/tubitos.jpg) no-repeat;
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h3>What should this page contain?</h3>
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                Project Description
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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                        <li><a href = "#project_title">Project Title</a></li>
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                        <li><a href = "#abstract_title">Abstract</a></li>
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<li><a href = "#desc_title">Background</a></li>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2016.igem.org/Team:Imperial_College/Description">2016 Imperial College</a></li>
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                    Chitinolytic Activity of Serratia Marcescens Chitinase in Response to Various Species of Pathological Fungi
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<li><a href="https://2016.igem.org/Team:Wageningen_UR/Description">2016 Wageningen UR</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> 2014 UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">2014 SYSU Software</a></li>
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                    Abstract
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                    Fungi producing harmful mycotoxins flourish on various crops. Such fungal infections significantly reduce sustainability and food production in developing countries, where mycotoxin exposure from lack of advanced food storage are responsible for severe economic losses and 40% of diseases. Our team developed a modified enzyme chitinase capable of breaking down chitin cell walls.  Specifically, Serratia Marcescens Chitinase works against multiple families of fungi. By GSTChiA Chitinase genes with a signal sequence from araC, we successfully generated an Escherichia coli line that secretes chitinase against Rhizoctoniasolani Solani, Alternaria raphani, and many other pathogenic fungi. Expression of GSTChiA was further quantified through analysis of chitin compounds. This project will provide an easily accessible method capable of combating major pathogens, saving crop yield and revenue.
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    Background
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.  
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    Fungi producing harmful mycotoxins flourish on various crops. Such fungal infections significantly reduce sustainability and food production in developing countries and in agricultural industry, in which mycotoxins deplete inadequately preserved food storages and cause 40% of diseases. Chitin is the second most common polysaccharide found in nature, next to cellulose, and is an important component of fungal cell wall. Chitinase is an enzyme that actively breaks down chitin, and different strains of chitinase are optimized to effectively degrade the cell walls of different strains of chitin. Serratia Marcescens Chitinase acts against Rhizoctonia solani, Bipolaris sp, Alternaria raphani, Alternaria brassicicola, and Fusarium oxysporum which impact a wide range of agricultural products including various herbs, cruciferous vegetables, radish species, bananas, and potatoes. The construct of this project combines a ChiA sequence from Serratia Marcescens and a Glutathione S-Transferase (GST) sequence making it is possible to easily pull down protein and produce ChiA on a small scale. Finally, Serratia Marcescen Chitinase-- when combined with the GST, FLAG-tag, and a pBAD vector-- can be mutated via Error Prone PCR, as proven in this experiment, meaning that it is possible to evolve the enzyme via this process.
 
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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Revision as of 23:12, 10 October 2018

Project Description

Project Description

Chitinolytic Activity of Serratia Marcescens Chitinase in Response to Various Species of Pathological Fungi

Abstract

Fungi producing harmful mycotoxins flourish on various crops. Such fungal infections significantly reduce sustainability and food production in developing countries, where mycotoxin exposure from lack of advanced food storage are responsible for severe economic losses and 40% of diseases. Our team developed a modified enzyme chitinase capable of breaking down chitin cell walls. Specifically, Serratia Marcescens Chitinase works against multiple families of fungi. By GSTChiA Chitinase genes with a signal sequence from araC, we successfully generated an Escherichia coli line that secretes chitinase against Rhizoctoniasolani Solani, Alternaria raphani, and many other pathogenic fungi. Expression of GSTChiA was further quantified through analysis of chitin compounds. This project will provide an easily accessible method capable of combating major pathogens, saving crop yield and revenue.

Background

Fungi producing harmful mycotoxins flourish on various crops. Such fungal infections significantly reduce sustainability and food production in developing countries and in agricultural industry, in which mycotoxins deplete inadequately preserved food storages and cause 40% of diseases. Chitin is the second most common polysaccharide found in nature, next to cellulose, and is an important component of fungal cell wall. Chitinase is an enzyme that actively breaks down chitin, and different strains of chitinase are optimized to effectively degrade the cell walls of different strains of chitin. Serratia Marcescens Chitinase acts against Rhizoctonia solani, Bipolaris sp, Alternaria raphani, Alternaria brassicicola, and Fusarium oxysporum which impact a wide range of agricultural products including various herbs, cruciferous vegetables, radish species, bananas, and potatoes. The construct of this project combines a ChiA sequence from Serratia Marcescens and a Glutathione S-Transferase (GST) sequence making it is possible to easily pull down protein and produce ChiA on a small scale. Finally, Serratia Marcescen Chitinase-- when combined with the GST, FLAG-tag, and a pBAD vector-- can be mutated via Error Prone PCR, as proven in this experiment, meaning that it is possible to evolve the enzyme via this process.