Difference between revisions of "Team:OUC-China/miniToe"
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| − | <h2 class="major"><span> | + | <h2 class="major"><span>Design</span></h2> |
| + | <p> | ||
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| + | <h3>Reactions</h3> | ||
| + | <br />We can describe our miniToe system to be followings: | ||
| − | < | + | <div> |
| + | \[\rightarrow [𝑐𝑟𝑅𝑁𝐴 − 𝑅𝐵𝑆 − 𝑚𝑅𝑁𝐴_{𝑔𝑓𝑝}]\] | ||
| + | \[\rightarrow [𝑚𝑅𝑁𝐴_{Csy4}]\] | ||
| + | \[[𝑚𝑅𝑁𝐴_{𝐶𝑠𝑦4}]\rightarrow [𝑚𝑅𝑁𝐴_{𝐶𝑠𝑦4}] + [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}]\] | ||
| + | \[[Protein_{𝐶𝑠𝑦4}]+[crRNA-RBS-mRNA_{gfp}]\leftrightarrow [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}-crRNA-RBS-𝑚𝑅𝑁𝐴_{𝑔𝑓𝑝}]\] | ||
| + | \[[Protein_{𝐶𝑠𝑦4}-crRNA-RBS-mRNA_{gfp}]\rightarrow [𝑚𝑅𝑁𝐴_{gfp}] + [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}-𝑐𝑟𝑅𝑁𝐴]\] | ||
| + | \[[𝑚𝑅𝑁𝐴_{gfp}]\rightarrow [𝑚𝑅𝑁𝐴_{gfp}] + [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{gfp}]\] | ||
| + | \[[crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\] | ||
| + | \[[𝑚𝑅𝑁𝐴_{𝐶𝑠𝑦4}]\rightarrow \emptyset\] | ||
| + | \[[𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}]\rightarrow \emptyset\] | ||
| + | \[[Protein_{𝐶𝑠𝑦4}-crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\] | ||
| + | \[[𝑚𝑅𝑁𝐴_{gfp}]\rightarrow \emptyset\] | ||
| + | \[[𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}-𝑐𝑟𝑅𝑁𝐴]\rightarrow \emptyset\] | ||
| + | \[[𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{gfp}]\rightarrow \emptyset\] | ||
| + | </div> | ||
| + | <br /><br />Two equations, describing the functional binding and cleavage of Csy4 protein in biology, and three parameters,\[𝑘_{on}\] ,\[𝑘_{off}\] ,\[𝑘_{obs}\] , describing the same things in mathematics, are the core of our model. | ||
| + | <div align="center"><img src="https://static.igem.org/mediawiki/2018/9/90/T--OUC-China--design1-1.png" height="450"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/1/16/T--OUC-China--design1-2.png" height="450"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/3/3c/T--OUC-China--design1-3.png" height="450"> | ||
| + | </div> | ||
| + | <div align="center"><p>    Fig.1-1 The structure of Csy4         | ||
| + | Fig.1-2 The structure 22nt hairpin which can be recognized by Csy4.       | ||
| + | Fig.1-3 The Csy4/RNA complex.</p></div> | ||
| + | Also, | ||
| + | （crispr家族蛋白的背景：相较与其他crispr家族其他蛋白质的优点） | ||
| + | </p> | ||
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| + | </section> | ||
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</section> | </section> | ||
Revision as of 23:25, 10 October 2018
Design
Reactions
We can describe our miniToe system to be followings:
\[\rightarrow [𝑐𝑟𝑅𝑁𝐴 − 𝑅𝐵𝑆 − 𝑚𝑅𝑁𝐴_{𝑔𝑓𝑝}]\]
\[\rightarrow [𝑚𝑅𝑁𝐴_{Csy4}]\]
\[[𝑚𝑅𝑁𝐴_{𝐶𝑠𝑦4}]\rightarrow [𝑚𝑅𝑁𝐴_{𝐶𝑠𝑦4}] + [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}]\]
\[[Protein_{𝐶𝑠𝑦4}]+[crRNA-RBS-mRNA_{gfp}]\leftrightarrow [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}-crRNA-RBS-𝑚𝑅𝑁𝐴_{𝑔𝑓𝑝}]\]
\[[Protein_{𝐶𝑠𝑦4}-crRNA-RBS-mRNA_{gfp}]\rightarrow [𝑚𝑅𝑁𝐴_{gfp}] + [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}-𝑐𝑟𝑅𝑁𝐴]\]
\[[𝑚𝑅𝑁𝐴_{gfp}]\rightarrow [𝑚𝑅𝑁𝐴_{gfp}] + [𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{gfp}]\]
\[[crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\]
\[[𝑚𝑅𝑁𝐴_{𝐶𝑠𝑦4}]\rightarrow \emptyset\]
\[[𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}]\rightarrow \emptyset\]
\[[Protein_{𝐶𝑠𝑦4}-crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\]
\[[𝑚𝑅𝑁𝐴_{gfp}]\rightarrow \emptyset\]
\[[𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{𝐶𝑠𝑦4}-𝑐𝑟𝑅𝑁𝐴]\rightarrow \emptyset\]
\[[𝑃𝑟𝑜𝑡𝑒𝑖𝑛_{gfp}]\rightarrow \emptyset\]
Two equations, describing the functional binding and cleavage of Csy4 protein in biology, and three parameters,\[𝑘_{on}\] ,\[𝑘_{off}\] ,\[𝑘_{obs}\] , describing the same things in mathematics, are the core of our model.
Fig.1-1 The structure of Csy4 Fig.1-2 The structure 22nt hairpin which can be recognized by Csy4. Fig.1-3 The Csy4/RNA complex.
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