Difference between revisions of "Team:OUC-China/miniToe"

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<p>
  
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<h3>Reactions</h3>
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<br />We can describe our miniToe system to be followings:
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<div>
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            \[\rightarrow [๐‘๐‘Ÿ๐‘…๐‘๐ด โˆ’ ๐‘…๐ต๐‘† โˆ’ ๐‘š๐‘…๐‘๐ด_{๐‘”๐‘“๐‘}]\]
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                        \[\rightarrow [๐‘š๐‘…๐‘๐ด_{Csy4}]\]
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                      \[[๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow [๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}]\]
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                        \[[Protein_{๐ถ๐‘ ๐‘ฆ4}]+[crRNA-RBS-mRNA_{gfp}]\leftrightarrow [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-๐‘š๐‘…๐‘๐ด_{๐‘”๐‘“๐‘}]\]
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                        \[[Protein_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-mRNA_{gfp}]\rightarrow [๐‘š๐‘…๐‘๐ด_{gfp}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-๐‘๐‘Ÿ๐‘…๐‘๐ด]\]
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                        \[[๐‘š๐‘…๐‘๐ด_{gfp}]\rightarrow [๐‘š๐‘…๐‘๐ด_{gfp}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{gfp}]\]
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\[[crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\]
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                    \[[๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow \emptyset\]
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                    \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow \emptyset\]
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                        \[[Protein_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\]
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\[[๐‘š๐‘…๐‘๐ด_{gfp}]\rightarrow \emptyset\]
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\[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-๐‘๐‘Ÿ๐‘…๐‘๐ด]\rightarrow \emptyset\]
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                    \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{gfp}]\rightarrow \emptyset\]
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</div>
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<br /><br />Two equations, describing the functional binding and cleavage of Csy4 protein in biology, and three parameters,๐‘˜_{on},\[๐‘˜_{off}\] ,\[๐‘˜_{obs}\] , describing the same things in mathematics, are the core of our model.
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<div align="center"><img src="https://static.igem.org/mediawiki/2018/9/90/T--OUC-China--design1-1.png" height="450">
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                    <img src="https://static.igem.org/mediawiki/2018/1/16/T--OUC-China--design1-2.png"  height="450">
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<img src="https://static.igem.org/mediawiki/2018/3/3c/T--OUC-China--design1-3.png"  height="450">
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</div>
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<div align="center"><p>&emsp;&emsp;&emsp;&emsp;Fig.1-1 The structure of Csy4  &emsp;&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;
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Fig.1-2 The structure 22nt hairpin which can be recognized by Csy4.&emsp;&emsp;&emsp;&emsp;&emsp;&emsp;
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Fig.1-3 The Csy4/RNA complex.</p></div>
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Also,
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๏ผˆcrisprๅฎถๆ—่›‹็™ฝ็š„่ƒŒๆ™ฏ๏ผš็›ธ่พƒไธŽๅ…ถไป–crisprๅฎถๆ—ๅ…ถไป–่›‹็™ฝ่ดจ็š„ไผ˜็‚น๏ผ‰
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</p>
 
 
 
 

Revision as of 23:26, 10 October 2018

Team OUC-China: Main
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Design

Reactions


We can describe our miniToe system to be followings:
\[\rightarrow [๐‘๐‘Ÿ๐‘…๐‘๐ด โˆ’ ๐‘…๐ต๐‘† โˆ’ ๐‘š๐‘…๐‘๐ด_{๐‘”๐‘“๐‘}]\] \[\rightarrow [๐‘š๐‘…๐‘๐ด_{Csy4}]\] \[[๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow [๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}]\] \[[Protein_{๐ถ๐‘ ๐‘ฆ4}]+[crRNA-RBS-mRNA_{gfp}]\leftrightarrow [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-๐‘š๐‘…๐‘๐ด_{๐‘”๐‘“๐‘}]\] \[[Protein_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-mRNA_{gfp}]\rightarrow [๐‘š๐‘…๐‘๐ด_{gfp}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-๐‘๐‘Ÿ๐‘…๐‘๐ด]\] \[[๐‘š๐‘…๐‘๐ด_{gfp}]\rightarrow [๐‘š๐‘…๐‘๐ด_{gfp}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{gfp}]\] \[[crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\] \[[๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow \emptyset\] \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow \emptyset\] \[[Protein_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\] \[[๐‘š๐‘…๐‘๐ด_{gfp}]\rightarrow \emptyset\] \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-๐‘๐‘Ÿ๐‘…๐‘๐ด]\rightarrow \emptyset\] \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{gfp}]\rightarrow \emptyset\]


Two equations, describing the functional binding and cleavage of Csy4 protein in biology, and three parameters,\[๐‘˜_{on}\] ,\[๐‘˜_{off}\] ,\[๐‘˜_{obs}\] , describing the same things in mathematics, are the core of our model.

    Fig.1-1 The structure of Csy4         Fig.1-2 The structure 22nt hairpin which can be recognized by Csy4.       Fig.1-3 The Csy4/RNA complex.

Also, ๏ผˆcrisprๅฎถๆ—่›‹็™ฝ็š„่ƒŒๆ™ฏ๏ผš็›ธ่พƒไธŽๅ…ถไป–crisprๅฎถๆ—ๅ…ถไป–่›‹็™ฝ่ดจ็š„ไผ˜็‚น๏ผ‰

Reactions


We can describe our miniToe system to be followings:
\[\rightarrow [๐‘๐‘Ÿ๐‘…๐‘๐ด โˆ’ ๐‘…๐ต๐‘† โˆ’ ๐‘š๐‘…๐‘๐ด_{๐‘”๐‘“๐‘}]\] \[\rightarrow [๐‘š๐‘…๐‘๐ด_{Csy4}]\] \[[๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow [๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}]\] \[[Protein_{๐ถ๐‘ ๐‘ฆ4}]+[crRNA-RBS-mRNA_{gfp}]\leftrightarrow [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-๐‘š๐‘…๐‘๐ด_{๐‘”๐‘“๐‘}]\] \[[Protein_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-mRNA_{gfp}]\rightarrow [๐‘š๐‘…๐‘๐ด_{gfp}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-๐‘๐‘Ÿ๐‘…๐‘๐ด]\] \[[๐‘š๐‘…๐‘๐ด_{gfp}]\rightarrow [๐‘š๐‘…๐‘๐ด_{gfp}] + [๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{gfp}]\] \[[crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\] \[[๐‘š๐‘…๐‘๐ด_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow \emptyset\] \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}]\rightarrow \emptyset\] \[[Protein_{๐ถ๐‘ ๐‘ฆ4}-crRNA-RBS-mRNA_{gfp}]\rightarrow \emptyset\] \[[๐‘š๐‘…๐‘๐ด_{gfp}]\rightarrow \emptyset\] \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{๐ถ๐‘ ๐‘ฆ4}-๐‘๐‘Ÿ๐‘…๐‘๐ด]\rightarrow \emptyset\] \[[๐‘ƒ๐‘Ÿ๐‘œ๐‘ก๐‘’๐‘–๐‘›_{gfp}]\rightarrow \emptyset\]


Two equations, describing the functional binding and cleavage of Csy4 protein in biology, and three parameters,๐‘˜_{on},\[๐‘˜_{off}\] ,\[๐‘˜_{obs}\] , describing the same things in mathematics, are the core of our model.

    Fig.1-1 The structure of Csy4         Fig.1-2 The structure 22nt hairpin which can be recognized by Csy4.       Fig.1-3 The Csy4/RNA complex.

Also, ๏ผˆcrisprๅฎถๆ—่›‹็™ฝ็š„่ƒŒๆ™ฏ๏ผš็›ธ่พƒไธŽๅ…ถไป–crisprๅฎถๆ—ๅ…ถไป–่›‹็™ฝ่ดจ็š„ไผ˜็‚น๏ผ‰

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