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| − | ">INTERLAB STUDY</h3> | + | ">Biological Design: The Golden Braid Assembly</h3> |
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| − | ">INTRODUCTION</h4>
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| − | <!--<h5 style=" | + | <p>We are continuously talking about a machine which can create its <b>own genetic circuits</b>, by using pre-designed parts, and ‘print’ them inside different living cell chassis. But how is Printeria going to perform all these complex reactions?</p> |
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| − | <!--<h6 style=" | + | <p>One of the first attempts to standardize a restriction enzyme-based DNA assembly method was BioBricks (1). However, its pairwise nature can make the construction of multipart systems, such as transcriptional units, time-consuming.</p> |
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| + | <p>Printeria is using a state-of-the-art technology based on the Golden Gate Assembly, the <b>Golden Braid Assembly Method</b>. This technology uses <b>type IIs restriction enzymes</b> in order to cut all the parts and build these genetic circuits.</p> |
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| + | <p><b>The Golden Gate assembly is based on type IIs enzymes. But what does this really mean? </b></p> |
| | + | <p>Type IIs restriction enzymes are a group of endonucleases that recognize <b>asymmetric double stranded DNA </b>sequences and <b>cleave outside</b> of their recognition sequence. Thus, digestion leaves short <b>single stranded overhangs</b> with non-specific sequences. </p> |
| | + | <p>This allows us to design the cleaving region so that we are creating a sticky end that will be pasted with the following part, and so on. This is the way in which <b>directionality</b> is maintained and parts are pasted in the desired order.</p> |
| | + | <p><b>But why is this assembly technique so crucial for our machine to work?</b></p> |
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| − | margin-bottom: 0; | + | <p>Carefully positioning the recognition and cleavage sites, in opposite directions, for the entry and destination vectors leads into a <b>final plasmid</b> - once the DNA construction has been ligated -where there is <b>no recognition site</b>. So, once the insert has been ligated, it cannot be cut again. This allows simultaneous digestion and ligation in <b>a one-pot reaction</b> so that the whole assembly is taking place in a single step. This fact makes the Golden Braid Technology perfect for our machine to work, as the whole reaction should take place in a single droplet.</p> |
| − | color: #353535;">Do you imagine doing an experiment that could not be repeated? What if, after performing the same experiment several times, you obtain different results each time? This is a common problem throughout almost all laboratories in the entire world. A challenge, not just for Synthetic Biology but for any type of science, is taking reliable and repeatable measurements.
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| | + | <p><b>High efficiency</b>. By means of modifying the different parameters we can end up with an almost 100% efficiency.</p> |
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| | + | <li> |
| | + | <p><b>Robust reaction</b>. The moving of the droplet across the PCB surface should not be a real problem for it to work.</p> |
| | + | </li> |
| | + | <li> |
| | + | <p>The ability of cutting and pasting several parts by using the same enzymes makes the whole <b>assembly easier to perform</b>.</p> |
| | + | </li> |
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| | + | <p><b>No scars</b> are left when assembling the different parts.</p> |
| | + | </li> |
| | + | </ul> |
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| − | </p> | + | <h4>The Golden Braid Assembly</h4> |
| − | <p style=" | + | <p>In the GB assembly method the transcriptional units can be combined in <b>binary steps</b> to grow <b>multigene structures</b> (several TUs within the same destination plasmid). To do so, this system relies on the switching between two levels of plasmids, <b>α and Ω</b> , with different antibiotic resistance. |
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| − | color: #353535;">Over the past four years, the iGem Measurement Committee has been developing a series of experiments to make the biggest interlaboratory studies ever done in synthetic biology, and, in that way, try to fix all possible variables within a particular protocol.
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| | + | <p>This Technology can mainly be divided into three different complexity levels:</p> |
| | + | <h4>Level 0 Assembly</h4> |
| | + | <p>This is the easiest Golden Braid reaction. It implies the removal of internal restriction sites for the enzymes used in GB (<b>BsaI, BsmBI</b>) and the addition of appropriate 4-nt flanking overhangs to convert a single basic part (promoter, RBS, CDS or terminator) into a standard part inside a predesigned vector (<b>domestication to the GB grammar</b>). </p> |
| | + | <p>We are using this level 0 assembly in the lab, so that we domesticate every single part which Printeria will use to create its own transcriptional units. </p> |
| | + | <p>The goal is to end up with a series of plasmids that contain each of the different promoters, RBSs, CDSs and terminators.</p> |
| | + | <p>In our specific case, sticky ends of the parts are predesigned so that when cleaving our domestication vector pUD2 with BsmBI, they are pasted in a proper way.</p> |
| | + | <p>This <b>pUD2</b> plasmid has a<b> chloramphenicol resistance</b> and the <b>lacZ cassette</b> so that blue-white screening can be performed among the transformed E. coli cells.</p> |
| | + | <p>This can be thought as <b>Printeria’s PAST</b>.</p> |
| | + | <img src="https://static.igem.org/mediawiki/2018/d/d6/T--Valencia_UPV--im5UPV2018.png" alt=""> |
| | + | <h6>Figure 1: P10500 domestication vector. Yellow and black puzzle-like pieces represent the restriction sites for BsmbI. It has chloramphenicol resistance.</h6> |
| | + | <img src="https://static.igem.org/mediawiki/2018/f/fc/T--Valencia_UPV--im6UPV2018.png" alt=""> |
| | + | <h6>Figure 2: Designing of the different basic parts. The upper sequence corresponds with the strand that was ordered for synthesis. The lower sequence represents the complementary strand. BsmbI restriction sites are represented by the yellow and black cuts. The coloured sequences represent BsaI restriction sites when the part is inserted in our domestication vector. A 6-nucleotide scar was added to the RBS so that the ribosome could bind. </h6> |
| | + | <div class="fotoConPie"> |
| | + | <div class="row" style="margin: 0;"> |
| | + | <div class="col-md-6" style="padding: 0;padding-right: 0.3em;"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/b/b2/T--Valencia_UPV--im7UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | </div> |
| | + | <div class="col-md-6" style="padding: 0;padding-left: 0.3em;"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/f/f5/T--Valencia_UPV--im8UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | </div> |
| | + | <div class="pieDeImagen"> |
| | + | <h6> |
| | + | Figure 3: BsmbI digested part and vector. |
| | + | </h6> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | <img src="https://static.igem.org/mediawiki/2018/2/28/T--Valencia_UPV--im9UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 4: Domestication of a promoter inside the P10500 </h6> |
| | + | <img src="https://static.igem.org/mediawiki/2018/7/7f/T--Valencia_UPV--im10UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 5: Basic domesticated part. Light yellow and grey sequences represent the BsmbI sticky ends which have been glued. As the new plasmid is assembled, BsaI restriction sites appear (blue and pink cuts). </h6> |
| | + | <img src="https://static.igem.org/mediawiki/2018/1/1e/T--Valencia_UPV--im11UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 6: All Golden Braid compatible domesticated parts. BsaI restriction sites appear. They are represented by the coloured puzzle-like pieces. </h6> |
| | + | <h4>Level 1 Assembly</h4> |
| | + | <p>This second level of complexity cannot be performed without having fulfilled the domestication of the parts. Once it is done, we can now create a <b>simple transcriptional unit</b>. This is what Printeria can assemble nowadays.</p> |
| | + | <p>As said before, each of these domesticated parts now has a BsaI recognition site and a cleaving site which, when cleaved, will match with the contiguous parts. In other words, promoters will stick with the left end of our destination vector, <b>pGreen alpha1 (kanr)</b>, using their left sticky end and with RBSs using their right end. At the same time CDSs will stick to these RBSs using their left sticky end, and to the terminators with their right end. Finally, the terminators will stick to the right end of our backbone destination vector so that, we will end up having a <b>plasmid with a single TU inside it</b>.</p> |
| | + | <p>This is <b>the PRESENT</b>.</p> |
| | + | <img src="https://static.igem.org/mediawiki/2018/d/d9/T--Valencia_UPV--im12UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 7: pGreen alpha 1 destination vector. The BsmbI restriction site will allow us to create a level 2 assembly.</h6> |
| | + | <div class="fotoConPie"> |
| | + | <div class="row" style="margin: 0;"> |
| | + | <div class="col-md-6" style="padding: 0;padding-right: 0.3em;"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/0/0e/T--Valencia_UPV--im13UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | </div> |
| | + | <div class="col-md-6" style="padding: 0;padding-left: 0.3em;"> |
| | + | <img src="https://static.igem.org/mediawiki/2018/1/16/T--Valencia_UPV--im14UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | </div> |
| | + | <div class="pieDeImagen"> |
| | + | <h6> |
| | + | Figure 8: BsaI digested destination and domesticated part to build a transcriptional unit. |
| | + | </h6> |
| | + | </div> |
| | + | </div> |
| | + | </div> |
| | + | <img src="https://static.igem.org/mediawiki/2018/9/93/T--Valencia_UPV--im15UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 9: Transcriptional unit assembly</h6> |
| | + | <img src="https://static.igem.org/mediawiki/2018/0/0d/T--Valencia_UPV--im16UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 10: TU insertion inside pGreen alpha1</h6> |
| | + | <img src="https://static.igem.org/mediawiki/2018/f/fd/T--Valencia_UPV--im17UPV2018.png" alt="" style="margin-top: 1.8em;margin-bottom: 0.8em;border-radius: 0.3em;"> |
| | + | <h6>Figure 11: Light coloured sequences represent the BsaI sticky ends which have been glued. As the new plasmid is assembled, BsmbI restriction sites appear (blue and dark blue) for a level 2 assembly.</h6> |
| | + | <h4>Level 2 Assembly</h4> |
| | + | <p>This is the last level of complexity in which, by using a combination of <b>α and Ω vectors</b>, we can <b>cut and paste several transcriptional units inside the same plasmid </b> so that more <b>complex genetic circuits</b> can be created. </p> |
| | + | <p>Printeria aims to arrive to this level of complexity someday so that its possibilities and <b>combinations are infinite</b>.</p> |
| | + | <p>This will be <b>Printeria’s FUTURE</b>.</p> |
| | + | <h4>References</h4> |
| | + | <ol> |
| | + | <li> |
| | + | <p>Shetty RP, Endy D, Knight TF. Engineering BioBrick vectors from BioBrick parts. J Biol Eng. 2008;2: 5.</p> |
| | + | </li> |
| | + | <li> |
| | + | <p>Andreou AI, Nakayama N (2018) Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly. PLOS ONE 13(1): e0189892. |
| | </p> | | </p> |
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| + | <p> Sarrion- Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández- del- Carmen A, et al. ( 2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLOS ONE 6( 7) : e21622. |
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| − | ">WHAT IS THIS YEAR'S GOAL?</h4>
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| − | To know if there is any chance to reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or c-forming units (CFUs) instead of optical density (OD).
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| + | <p>Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, Forment J, Ziarsolo P, et al. Golden- Braid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology. Plant Physiol. 2013; 162: 1618–1631</p> |
| − | color: #353535;"> In order to compute the cell count in our samples, we will use two orthogonal approaches:
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| − | ">Approach 1: Converting between absorbance of cells to absorbance of a known concentration of beads</h5>
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| − | The theory under how absorbance is measured is quite simple: a liquid sample of cells scatter light in a way or another depending on the number of cells this sample contains. The Committee provides us a sample with silica beads which are almost the same size and shape as a typical E. coli cell. So, when mixed with water, we obtain a liquid that should scatter light in a similar way as our E. coli sample does.
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| − | Because we know the concentration of beads, the absorbance measurement from a particular cell sample could be converted into an “equivalent concentration of beads” measurement, so that they are more universal and comparable measurements between different labs.
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| − | | + | <img src="https://static.igem.org/mediawiki/2018/6/69/T--Valencia_UPV--MerceríaPaquitaUPV2018.jpeg" |
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| − | ">Approach 2: Counting c-forming units (CFUs) from the sample</h5>
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| − | This method relies on the idea that every grown c in our plate comes from a single cell. So, if we spread a known cell culture volume over an agar plate and then we count the number of colonies, we should have an idea on how many cells our liquid sample had. We will have to determine the number of CFUs in positive and negative control samples in order to compute a conversion factor from absorbance to CFU.
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| − | ">PLATE READER SETUP</h4>
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| − | ">ABSORBANCE600</h5>
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| − | color: #353535;">Wavelengths: 600
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| − | color: #353535;">Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 8
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| − | ">FLUORESCENCE</h5>
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| − | <p style="
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| − | color: #353535;"> Excitation: 485, Emission: 528
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| − | color: #353535;"> Optics: Top, Gain: 50
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| − | color: #353535;"> Light Source: Xenon Flash, Lamp Energy: High
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| − | color: #353535;"> Read Speed: Normal, Delay: 100 msec, Measurements/Data Point: 10
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| − | ">USED PARTS</h4>
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| − | <div>
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| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
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| − | <thead><tr>
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| − | <th class="thHec">Device</th>
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| − | <th class="thHec">Part number</th>
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| − | <th class="thHec">Plate</th>
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| − | <th class="thHec">Location</th>
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| − | </tr>
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| − | </thead><tbody><tr>
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| − | <td class="tdHec">Negative control</td>
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| − | <td class="tdHec">BBa_R0040</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2D</td>
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| − | </tr>
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| − | <tr>
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| − | <td class="tdHec">Positive control</td>
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| − | <td class="tdHec">BBa_I20270</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2B</td>
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| − | </tr>
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| − | <td class="tdHec">Test Device 1</td>
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| − | <td class="tdHec">BBa_J364000</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2F</td>
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| − | </tr>
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| − | <td class="tdHec">Test Device 2</td>
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| − | <td class="tdHec">BBa_J364001</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2H</td>
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| − | </tr>
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| − | <td class="tdHec">Test Device 3</td>
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| − | <td class="tdHec">BBa_J364002</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2J</td>
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| − | </tr>
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| − | <td class="tdHec">Test Device 4</td>
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| − | <td class="tdHec">BBa_J364007</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2L</td>
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| − | </tr>
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| − | <td class="tdHec">Test Device 5</td>
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| − | <td class="tdHec">BBa_J364008</td>
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| − | <td class="tdHec">Kit Plate 7</td>
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| − | <td class="tdHec">Well 2N</td>
| + | |
| − | </tr>
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| − | <td class="tdHec">Test Device 6</td>
| + | |
| − | <td class="tdHec">BBa_J364009</td>
| + | |
| − | <td class="tdHec">Kit Plate 7</td>
| + | |
| − | <td class="tdHec">Well 2P</td>
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">CALIBRATION 1: OD600 REFERENCE POINT</h4>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Using LUDOX CL-X as a point reference to obtain a conversion factor to transform our absorbance (Abs600) data from our plate reader into a comparable OD 600 measurement as would be obtained in a spectrophotometer.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec"></th>
| + | |
| − | <th class="thHec">LUDOX CL-X</th>
| + | |
| − | <th class="thHec">H2O</th>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">R1</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.051</td>
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">R2</td>
| + | |
| − | <td class="tdHec">0.060</td>
| + | |
| − | <td class="tdHec">0.049</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">R3</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.043</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">R4</td>
| + | |
| − | <td class="tdHec">0.062</td>
| + | |
| − | <td class="tdHec">0.049</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Arith. Mean</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.048</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Corrected Abs600</td>
| + | |
| − | <td class="tdHec">0.013</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">Reference OD600</td>
| + | |
| − | <td class="tdHec">0.063
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <td class="tdHec">OD600/Abs600</td>
| + | |
| − | <td class="tdHec">4.846</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">CALIBRATION 2: PARTICLE STANDARD CURVE</h4>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> This allows us to construct a standard curve of particle concentration which can be used to convert Abs 600 measurements to an estimated number of cells.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">CALIBRATION 3: FLUORESCENCE STANDARD CURVE</h4>
| + | |
| − | <p style="
| + | |
| − | line-height: 1.7;
| + | |
| − | margin-bottom: 0;
| + | |
| − | color: #353535;"> Absolute fluorescence values cannot be directly compared from one instrument to another. In order to compare fluorescence output of test devices between teams, it is necessary for each team to create a standard fluorescence curve.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <h4 style="
| + | |
| − | color: #353535;
| + | |
| − | font-weight: 700;
| + | |
| − | font-size: 21px;
| + | |
| − | margin-bottom: 0.8em;
| + | |
| − | ">EXPERIMENT</h4>
| + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">Fluorescence Raw Readings:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">3618</td>
| + | |
| − | <td class="tdHec">3087</td>
| + | |
| − | <td class="tdHec">5355</td>
| + | |
| − | <td class="tdHec">2950</td>
| + | |
| − | <td class="tdHec">3402</td>
| + | |
| − | <td class="tdHec">4799</td>
| + | |
| − | <td class="tdHec">4112</td>
| + | |
| − | <td class="tdHec">3901</td>
| + | |
| − | <td class="tdHec">3350</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">3648</td>
| + | |
| − | <td class="tdHec">3414</td>
| + | |
| − | <td class="tdHec">5158</td>
| + | |
| − | <td class="tdHec">2887</td>
| + | |
| − | <td class="tdHec">3260</td>
| + | |
| − | <td class="tdHec">4647</td>
| + | |
| − | <td class="tdHec">4159</td>
| + | |
| − | <td class="tdHec">3758</td>
| + | |
| − | <td class="tdHec">3193</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">3273</td>
| + | |
| − | <td class="tdHec">3442</td>
| + | |
| − | <td class="tdHec">5077</td>
| + | |
| − | <td class="tdHec">1611</td>
| + | |
| − | <td class="tdHec">3331</td>
| + | |
| − | <td class="tdHec">4607</td>
| + | |
| − | <td class="tdHec">3972</td>
| + | |
| − | <td class="tdHec">3793</td>
| + | |
| − | <td class="tdHec">3234</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">3301</td>
| + | |
| − | <td class="tdHec">1381</td>
| + | |
| − | <td class="tdHec">5307</td>
| + | |
| − | <td class="tdHec">1074</td>
| + | |
| − | <td class="tdHec">3446</td>
| + | |
| − | <td class="tdHec">4519</td>
| + | |
| − | <td class="tdHec">4416</td>
| + | |
| − | <td class="tdHec">3804</td>
| + | |
| − | <td class="tdHec">3221</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">3350</td>
| + | |
| − | <td class="tdHec">3537</td>
| + | |
| − | <td class="tdHec">5091</td>
| + | |
| − | <td class="tdHec">3702</td>
| + | |
| − | <td class="tdHec">3976</td>
| + | |
| − | <td class="tdHec">4621</td>
| + | |
| − | <td class="tdHec">4517</td>
| + | |
| − | <td class="tdHec">3969</td>
| + | |
| − | <td class="tdHec">3256</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">3262</td>
| + | |
| − | <td class="tdHec">3409</td>
| + | |
| − | <td class="tdHec">4758</td>
| + | |
| − | <td class="tdHec">3623</td>
| + | |
| − | <td class="tdHec">3660</td>
| + | |
| − | <td class="tdHec">4208</td>
| + | |
| − | <td class="tdHec">4679</td>
| + | |
| − | <td class="tdHec">3867</td>
| + | |
| − | <td class="tdHec">3265</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">3255</td>
| + | |
| − | <td class="tdHec">3401</td>
| + | |
| − | <td class="tdHec">4784</td>
| + | |
| − | <td class="tdHec">3620</td>
| + | |
| − | <td class="tdHec">3662</td>
| + | |
| − | <td class="tdHec">4248</td>
| + | |
| − | <td class="tdHec">4481</td>
| + | |
| − | <td class="tdHec">3951</td>
| + | |
| − | <td class="tdHec">3231</td>
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">3225</td>
| + | |
| − | <td class="tdHec">3020</td>
| + | |
| − | <td class="tdHec">4855</td>
| + | |
| − | <td class="tdHec">3518</td>
| + | |
| − | <td class="tdHec">3672</td>
| + | |
| − | <td class="tdHec">4451</td>
| + | |
| − | <td class="tdHec">4281</td>
| + | |
| − | <td class="tdHec">4183</td>
| + | |
| − | <td class="tdHec">4070</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">4087</td>
| + | |
| − | <td class="tdHec">23103</td>
| + | |
| − | <td class="tdHec">44980</td>
| + | |
| − | <td class="tdHec">7751</td>
| + | |
| − | <td class="tdHec">4312</td>
| + | |
| − | <td class="tdHec">29208</td>
| + | |
| − | <td class="tdHec">6183</td>
| + | |
| − | <td class="tdHec">10720</td>
| + | |
| − | <td class="tdHec">3306</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">4238</td>
| + | |
| − | <td class="tdHec">23435</td>
| + | |
| − | <td class="tdHec">46319</td>
| + | |
| − | <td class="tdHec">7594</td>
| + | |
| − | <td class="tdHec">4341</td>
| + | |
| − | <td class="tdHec">28505</td>
| + | |
| − | <td class="tdHec">6522</td>
| + | |
| − | <td class="tdHec">10895</td>
| + | |
| − | <td class="tdHec">3177</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">4194</td>
| + | |
| − | <td class="tdHec">23871</td>
| + | |
| − | <td class="tdHec">45601</td>
| + | |
| − | <td class="tdHec">7757</td>
| + | |
| − | <td class="tdHec">4371</td>
| + | |
| − | <td class="tdHec">28765</td>
| + | |
| − | <td class="tdHec">6359</td>
| + | |
| − | <td class="tdHec">10515</td>
| + | |
| − | <td class="tdHec">3260</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">4195</td>
| + | |
| − | <td class="tdHec">24568</td>
| + | |
| − | <td class="tdHec">45744</td>
| + | |
| − | <td class="tdHec">7709</td>
| + | |
| − | <td class="tdHec">4546</td>
| + | |
| − | <td class="tdHec">29372</td>
| + | |
| − | <td class="tdHec">6156</td>
| + | |
| − | <td class="tdHec">11334</td>
| + | |
| − | <td class="tdHec">3205</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">4398</td>
| + | |
| − | <td class="tdHec">11707</td>
| + | |
| − | <td class="tdHec">43194</td>
| + | |
| − | <td class="tdHec">17428</td>
| + | |
| − | <td class="tdHec">4756</td>
| + | |
| − | <td class="tdHec">28009</td>
| + | |
| − | <td class="tdHec">11866</td>
| + | |
| − | <td class="tdHec">10612</td>
| + | |
| − | <td class="tdHec">3269</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">4351</td>
| + | |
| − | <td class="tdHec">12443</td>
| + | |
| − | <td class="tdHec">43496</td>
| + | |
| − | <td class="tdHec">17570</td>
| + | |
| − | <td class="tdHec">4804</td>
| + | |
| − | <td class="tdHec">28545</td>
| + | |
| − | <td class="tdHec">11928</td>
| + | |
| − | <td class="tdHec">10545</td>
| + | |
| − | <td class="tdHec">3250</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">4291</td>
| + | |
| − | <td class="tdHec">11451</td>
| + | |
| − | <td class="tdHec">43156</td>
| + | |
| − | <td class="tdHec">17421</td>
| + | |
| − | <td class="tdHec">4796</td>
| + | |
| − | <td class="tdHec">28475</td>
| + | |
| − | <td class="tdHec">11726</td>
| + | |
| − | <td class="tdHec">10544</td>
| + | |
| − | <td class="tdHec">3295</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">4292</td>
| + | |
| − | <td class="tdHec">12396</td>
| + | |
| − | <td class="tdHec">42704</td>
| + | |
| − | <td class="tdHec">17353</td>
| + | |
| − | <td class="tdHec">4938</td>
| + | |
| − | <td class="tdHec">28869</td>
| + | |
| − | <td class="tdHec">11517</td>
| + | |
| − | <td class="tdHec">10546</td>
| + | |
| − | <td class="tdHec">3231</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">Abs600 Raw Readings:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.067</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.060</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − | <td class="tdHec">0.065</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.045</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.062</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.048</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.045</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.047</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.071</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.071</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">0.055</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.056</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − | <td class="tdHec">0.049</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | <th class="thHec">LB+Chlor (blank)</th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.595</td>
| + | |
| − | <td class="tdHec">0.513</td>
| + | |
| − | <td class="tdHec">0.471</td>
| + | |
| − | <td class="tdHec">0.391</td>
| + | |
| − | <td class="tdHec">0.554</td>
| + | |
| − | <td class="tdHec">0.518</td>
| + | |
| − | <td class="tdHec">0.083</td>
| + | |
| − | <td class="tdHec">0.487</td>
| + | |
| − | <td class="tdHec">0.066</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.567</td>
| + | |
| − | <td class="tdHec">0.527</td>
| + | |
| − | <td class="tdHec">0.508</td>
| + | |
| − | <td class="tdHec">0.363</td>
| + | |
| − | <td class="tdHec">0.556</td>
| + | |
| − | <td class="tdHec">0.519</td>
| + | |
| − | <td class="tdHec">0.084</td>
| + | |
| − | <td class="tdHec">0.501</td>
| + | |
| − | <td class="tdHec">0.053</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.566</td>
| + | |
| − | <td class="tdHec">0.524</td>
| + | |
| − | <td class="tdHec">0.479</td>
| + | |
| − | <td class="tdHec">0.381</td>
| + | |
| − | <td class="tdHec">0.566</td>
| + | |
| − | <td class="tdHec">0.501</td>
| + | |
| − | <td class="tdHec">0.083</td>
| + | |
| − | <td class="tdHec">0.490</td>
| + | |
| − | <td class="tdHec">0.059</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.580</td>
| + | |
| − | <td class="tdHec">0.570</td>
| + | |
| − | <td class="tdHec">0.530</td>
| + | |
| − | <td class="tdHec">0.373</td>
| + | |
| − | <td class="tdHec">0.549</td>
| + | |
| − | <td class="tdHec">0.559</td>
| + | |
| − | <td class="tdHec">0.088</td>
| + | |
| − | <td class="tdHec">0.551</td>
| + | |
| − | <td class="tdHec">0.051</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.611</td>
| + | |
| − | <td class="tdHec">0.513</td>
| + | |
| − | <td class="tdHec">0.519</td>
| + | |
| − | <td class="tdHec">0.531</td>
| + | |
| − | <td class="tdHec">0.515</td>
| + | |
| − | <td class="tdHec">0.530</td>
| + | |
| − | <td class="tdHec">0.117</td>
| + | |
| − | <td class="tdHec">0.520</td>
| + | |
| − | <td class="tdHec">0.053</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">0.562</td>
| + | |
| − | <td class="tdHec">0.538</td>
| + | |
| − | <td class="tdHec">0.523</td>
| + | |
| − | <td class="tdHec">0.536</td>
| + | |
| − | <td class="tdHec">0.522</td>
| + | |
| − | <td class="tdHec">0.543</td>
| + | |
| − | <td class="tdHec">0.116</td>
| + | |
| − | <td class="tdHec">0.517</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.561</td>
| + | |
| − | <td class="tdHec">0.493</td>
| + | |
| − | <td class="tdHec">0.517</td>
| + | |
| − | <td class="tdHec">0.507</td>
| + | |
| − | <td class="tdHec">0.518</td>
| + | |
| − | <td class="tdHec">0.502</td>
| + | |
| − | <td class="tdHec">0.106</td>
| + | |
| − | <td class="tdHec">0.488</td>
| + | |
| − | <td class="tdHec">0.054</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">0.555</td>
| + | |
| − | <td class="tdHec">0.537</td>
| + | |
| − | <td class="tdHec">0.498</td>
| + | |
| − | <td class="tdHec">0.512</td>
| + | |
| − | <td class="tdHec">0.533</td>
| + | |
| − | <td class="tdHec">0.508</td>
| + | |
| − | <td class="tdHec">0.101</td>
| + | |
| − | <td class="tdHec">0.491</td>
| + | |
| − | <td class="tdHec">0.06</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">uM Fluorescein/OD:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.415</td>
| + | |
| − | <td class="tdHec">-1.019</td>
| + | |
| − | <td class="tdHec">4.780</td>
| + | |
| − | <td class="tdHec">-1.377</td>
| + | |
| − | <td class="tdHec">0.134</td>
| + | |
| − | <td class="tdHec">3.742</td>
| + | |
| − | <td class="tdHec">2.362</td>
| + | |
| − | <td class="tdHec">1.423</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.940</td>
| + | |
| − | <td class="tdHec">0.685</td>
| + | |
| − | <td class="tdHec">5.075</td>
| + | |
| − | <td class="tdHec">-1.185</td>
| + | |
| − | <td class="tdHec">0.160</td>
| + | |
| − | <td class="tdHec">4.506</td>
| + | |
| − | <td class="tdHec">2.495</td>
| + | |
| − | <td class="tdHec">1.459</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.101</td>
| + | |
| − | <td class="tdHec">0.496</td>
| + | |
| − | <td class="tdHec">4.760</td>
| + | |
| − | <td class="tdHec">-25.149</td>
| + | |
| − | <td class="tdHec">0.150</td>
| + | |
| − | <td class="tdHec">3.273</td>
| + | |
| − | <td class="tdHec">2.079</td>
| + | |
| − | <td class="tdHec">1.237</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.155</td>
| + | |
| − | <td class="tdHec">-28.512</td>
| + | |
| − | <td class="tdHec">4.973</td>
| + | |
| − | <td class="tdHec">-22.179</td>
| + | |
| − | <td class="tdHec">0.536</td>
| + | |
| − | <td class="tdHec">3.352</td>
| + | |
| − | <td class="tdHec">3.086</td>
| + | |
| − | <td class="tdHec">1.291</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.324</td>
| + | |
| − | <td class="tdHec">0.968</td>
| + | |
| − | <td class="tdHec">4.739</td>
| + | |
| − | <td class="tdHec">1.536</td>
| + | |
| − | <td class="tdHec">1.716</td>
| + | |
| − | <td class="tdHec">4.700</td>
| + | |
| − | <td class="tdHec">3.908</td>
| + | |
| − | <td class="tdHec">2.210</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">-0.012</td>
| + | |
| − | <td class="tdHec">0.372</td>
| + | |
| − | <td class="tdHec">6.610</td>
| + | |
| − | <td class="tdHec">1.233</td>
| + | |
| − | <td class="tdHec">1.224</td>
| + | |
| − | <td class="tdHec">4.175</td>
| + | |
| − | <td class="tdHec">3.652</td>
| + | |
| − | <td class="tdHec">1.696</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.030</td>
| + | |
| − | <td class="tdHec">0.585</td>
| + | |
| − | <td class="tdHec">4.813</td>
| + | |
| − | <td class="tdHec">0.482</td>
| + | |
| − | <td class="tdHec">1.336</td>
| + | |
| − | <td class="tdHec">2.865</td>
| + | |
| − | <td class="tdHec">3.522</td>
| + | |
| − | <td class="tdHec">1.859</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">-3.741</td>
| + | |
| − | <td class="tdHec">-3.254</td>
| + | |
| − | <td class="tdHec">4.055</td>
| + | |
| − | <td class="tdHec">-2.138</td>
| + | |
| − | <td class="tdHec">-2.056</td>
| + | |
| − | <td class="tdHec">1.581</td>
| + | |
| − | <td class="tdHec">0.934</td>
| + | |
| − | <td class="tdHec">0.350</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">0.046</td>
| + | |
| − | <td class="tdHec">1.373</td>
| + | |
| − | <td class="tdHec">3.189</td>
| + | |
| − | <td class="tdHec">0.424</td>
| + | |
| − | <td class="tdHec">0.064</td>
| + | |
| − | <td class="tdHec">1.776</td>
| + | |
| − | <td class="tdHec">5.245</td>
| + | |
| − | <td class="tdHec">0.546</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">0.064</td>
| + | |
| − | <td class="tdHec">1.325</td>
| + | |
| − | <td class="tdHec">2.939</td>
| + | |
| − | <td class="tdHec">0.442</td>
| + | |
| − | <td class="tdHec">0.072</td>
| + | |
| − | <td class="tdHec">1.684</td>
| + | |
| − | <td class="tdHec">3.344</td>
| + | |
| − | <td class="tdHec">0.534</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">0.057</td>
| + | |
| − | <td class="tdHec">1.374</td>
| + | |
| − | <td class="tdHec">3.124</td>
| + | |
| − | <td class="tdHec">0.433</td>
| + | |
| − | <td class="tdHec">0.068</td>
| + | |
| − | <td class="tdHec">1.788</td>
| + | |
| − | <td class="tdHec">4.002</td>
| + | |
| − | <td class="tdHec">0.522</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">0.058</td>
| + | |
| − | <td class="tdHec">1.276</td>
| + | |
| − | <td class="tdHec">2.752</td>
| + | |
| − | <td class="tdHec">0.433</td>
| + | |
| − | <td class="tdHec">0.083</td>
| + | |
| − | <td class="tdHec">1.596</td>
| + | |
| − | <td class="tdHec">2.472</td>
| + | |
| − | <td class="tdHec">0.504</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">0.063</td>
| + | |
| − | <td class="tdHec">0.568</td>
| + | |
| − | <td class="tdHec">2.655</td>
| + | |
| − | <td class="tdHec">0.918</td>
| + | |
| − | <td class="tdHec">0.100</td>
| + | |
| − | <td class="tdHec">1.607</td>
| + | |
| − | <td class="tdHec">4.163</td>
| + | |
| − | <td class="tdHec">0.487</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">0.068</td>
| + | |
| − | <td class="tdHec">0.592</td>
| + | |
| − | <td class="tdHec">2.677</td>
| + | |
| − | <td class="tdHec">0.927</td>
| + | |
| − | <td class="tdHec">0.104</td>
| + | |
| − | <td class="tdHec">1.613</td>
| + | |
| − | <td class="tdHec">4.558</td>
| + | |
| − | <td class="tdHec">0.491</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">0.061</td>
| + | |
| − | <td class="tdHec">0.576</td>
| + | |
| − | <td class="tdHec">2.668</td>
| + | |
| − | <td class="tdHec">0.966</td>
| + | |
| − | <td class="tdHec">0.100</td>
| + | |
| − | <td class="tdHec">1.742</td>
| + | |
| − | <td class="tdHec">5.025</td>
| + | |
| − | <td class="tdHec">0.518</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">0.066</td>
| + | |
| − | <td class="tdHec">0.595</td>
| + | |
| − | <td class="tdHec">2.793</td>
| + | |
| − | <td class="tdHec">0.968</td>
| + | |
| − | <td class="tdHec">0.112</td>
| + | |
| − | <td class="tdHec">1.764</td>
| + | |
| − | <td class="tdHec">6.263</td>
| + | |
| − | <td class="tdHec">0.526</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <h5 style="
| + | |
| − | color: #353535;
| + | |
| − | font-size: 16px;
| + | |
| − | font-weight: 750;
| + | |
| − | ">MEFL/particle:</h5>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 0:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </th></tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">1.31E+05</td>
| + | |
| − | <td class="tdHec">-3.22E+05</td>
| + | |
| − | <td class="tdHec">1.51E+06</td>
| + | |
| − | <td class="tdHec">-4.35E+05</td>
| + | |
| − | <td class="tdHec">4.24E+04</td>
| + | |
| − | <td class="tdHec">1.18E+06</td>
| + | |
| − | <td class="tdHec">7.46E+05</td>
| + | |
| − | <td class="tdHec">4.50E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">2.97E+05</td>
| + | |
| − | <td class="tdHec">2.16E+05</td>
| + | |
| − | <td class="tdHec">1.60E+06</td>
| + | |
| − | <td class="tdHec">-3.75E+05</td>
| + | |
| − | <td class="tdHec">5.05E+04</td>
| + | |
| − | <td class="tdHec">1.42E+06</td>
| + | |
| − | <td class="tdHec">7.89E+05</td>
| + | |
| − | <td class="tdHec">4.61E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">3.18E+04</td>
| + | |
| − | <td class="tdHec">1.57E+05</td>
| + | |
| − | <td class="tdHec">1.50E+06</td>
| + | |
| − | <td class="tdHec">-7.95E+06</td>
| + | |
| − | <td class="tdHec">4.75E+04</td>
| + | |
| − | <td class="tdHec">1.03E+06</td>
| + | |
| − | <td class="tdHec">6.57E+05</td>
| + | |
| − | <td class="tdHec">3.91E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">4.90E+04</td>
| + | |
| − | <td class="tdHec">-9011826</td>
| + | |
| − | <td class="tdHec">1.57E+06</td>
| + | |
| − | <td class="tdHec">-7.01E+06</td>
| + | |
| − | <td class="tdHec">1.70E+05</td>
| + | |
| − | <td class="tdHec">1.06E+06</td>
| + | |
| − | <td class="tdHec">9.75E+05</td>
| + | |
| − | <td class="tdHec">4.08E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">1.02E+05</td>
| + | |
| − | <td class="tdHec">3.06E+05</td>
| + | |
| − | <td class="tdHec">1.50E+06</td>
| + | |
| − | <td class="tdHec">4.85E+05</td>
| + | |
| − | <td class="tdHec">5.43E+05</td>
| + | |
| − | <td class="tdHec">1.49E+06</td>
| + | |
| − | <td class="tdHec">1.24E+06</td>
| + | |
| − | <td class="tdHec">6.98E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">-3.67E+03</td>
| + | |
| − | <td class="tdHec">1.18E+05</td>
| + | |
| − | <td class="tdHec">2.09E+06</td>
| + | |
| − | <td class="tdHec">3.90E+05</td>
| + | |
| − | <td class="tdHec">3.87E+05</td>
| + | |
| − | <td class="tdHec">1.32E+06</td>
| + | |
| − | <td class="tdHec">1.15E+06</td>
| + | |
| − | <td class="tdHec">5.36E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">9.40E+03</td>
| + | |
| − | <td class="tdHec">1.85E+05</td>
| + | |
| − | <td class="tdHec">2.09E+06</td>
| + | |
| − | <td class="tdHec">1.52E+05</td>
| + | |
| − | <td class="tdHec">4.22E+05</td>
| + | |
| − | <td class="tdHec">9.06E+05</td>
| + | |
| − | <td class="tdHec">1.11E+06</td>
| + | |
| − | <td class="tdHec">5.88E+05</td>
| + | |
| − |
| + | |
| − | </tr> <tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">-1.18E+06</td>
| + | |
| − | <td class="tdHec">-1.03E+06</td>
| + | |
| − | <td class="tdHec">1.28E+06</td>
| + | |
| − | <td class="tdHec">-6.76E+05</td>
| + | |
| − | <td class="tdHec">-6.50E+05</td>
| + | |
| − | <td class="tdHec">3.73E+05</td>
| + | |
| − | <td class="tdHec">2.95E+05</td>
| + | |
| − | <td class="tdHec">1.11E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | <div>
| + | |
| − | <table class="border--round table--alternate-row tableHec" style="width:100%">
| + | |
| − | <thead><tr>
| + | |
| − | <th class="thHec">Hour 6:</th>
| + | |
| − | <th class="thHec">Neg. Control</th>
| + | |
| − | <th class="thHec">Pos. Control</th>
| + | |
| − | <th class="thHec">Device 1</th>
| + | |
| − | <th class="thHec">Device 2</th>
| + | |
| − | <th class="thHec">Device 3</th>
| + | |
| − | <th class="thHec">Device 4</th>
| + | |
| − | <th class="thHec">Device 5</th>
| + | |
| − | <th class="thHec">Device 6</th>
| + | |
| − | </tr>
| + | |
| − |
| + | |
| − | </thead><tbody><tr>
| + | |
| − | <td class="tdHec">C1, R1</td>
| + | |
| − | <td class="tdHec">1.45E+04</td>
| + | |
| − | <td class="tdHec">4.34E+05</td>
| + | |
| − | <td class="tdHec">1.01E+06</td>
| + | |
| − | <td class="tdHec">1.34E+05</td>
| + | |
| − | <td class="tdHec">2.02E+04</td>
| + | |
| − | <td class="tdHec">5.61E+05</td>
| + | |
| − | <td class="tdHec">1.66E+06</td>
| + | |
| − | <td class="tdHec">1.73E+05</td>
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | <tr>
| + | |
| − | <td class="tdHec">C1, R2</td>
| + | |
| − | <td class="tdHec">2.02E+04</td>
| + | |
| − | <td class="tdHec">4.19E+05</td>
| + | |
| − | <td class="tdHec">9.29E+05</td>
| + | |
| − | <td class="tdHec">1.40E+05</td>
| + | |
| − | <td class="tdHec">2.27E+04</td>
| + | |
| − | <td class="tdHec">5.32E+05</td>
| + | |
| − | <td class="tdHec">1.06E+06</td>
| + | |
| − | <td class="tdHec">1.69E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R3</td>
| + | |
| − | <td class="tdHec">1.80E+04</td>
| + | |
| − | <td class="tdHec">4.34E+05</td>
| + | |
| − | <td class="tdHec">9.87E+05</td>
| + | |
| − | <td class="tdHec">1.37E+05</td>
| + | |
| − | <td class="tdHec">2.15E+04</td>
| + | |
| − | <td class="tdHec">5.65E+05</td>
| + | |
| − | <td class="tdHec">1.26E+06</td>
| + | |
| − | <td class="tdHec">1.65E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C1, R4</td>
| + | |
| − | <td class="tdHec">1.83E+04</td>
| + | |
| − | <td class="tdHec">4.03E+05</td>
| + | |
| − | <td class="tdHec">8.70E+05</td>
| + | |
| − | <td class="tdHec">1.37E+05</td>
| + | |
| − | <td class="tdHec">2.64E+04</td>
| + | |
| − | <td class="tdHec">5.05E+05</td>
| + | |
| − | <td class="tdHec">7.81E+05</td>
| + | |
| − | <td class="tdHec">1.59E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R1</td>
| + | |
| − | <td class="tdHec">1.98E+04</td>
| + | |
| − | <td class="tdHec">1.80E+05</td>
| + | |
| − | <td class="tdHec">8.39E+05</td>
| + | |
| − | <td class="tdHec">2.90E+05</td>
| + | |
| − | <td class="tdHec">3.15E+04</td>
| + | |
| − | <td class="tdHec">5.08E+05</td>
| + | |
| − | <td class="tdHec">1.32E+06</td>
| + | |
| − | <td class="tdHec">1.54E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R2</td>
| + | |
| − | <td class="tdHec">2.14E+04</td>
| + | |
| − | <td class="tdHec">1.87E+05</td>
| + | |
| − | <td class="tdHec">8.46E+05</td>
| + | |
| − | <td class="tdHec">2.93E+05</td>
| + | |
| − | <td class="tdHec">3.27E+04</td>
| + | |
| − | <td class="tdHec">5.10E+05</td>
| + | |
| − | <td class="tdHec">1.44E+06</td>
| + | |
| − | <td class="tdHec">1.55E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R3</td>
| + | |
| − | <td class="tdHec">1.92E+04</td>
| + | |
| − | <td class="tdHec">1.82E+05</td>
| + | |
| − | <td class="tdHec">8.43E+0.5</td>
| + | |
| − | <td class="tdHec">3.05E+05</td>
| + | |
| − | <td class="tdHec">3.17E+04</td>
| + | |
| − | <td class="tdHec">5.51E+05</td>
| + | |
| − | <td class="tdHec">1.59E+06</td>
| + | |
| − | <td class="tdHec">1.64E+05</td>
| + | |
| − |
| + | |
| − | </tr><tr>
| + | |
| − | <td class="tdHec">C2, R4</td>
| + | |
| − | <td class="tdHec">2.10E+04</td>
| + | |
| − | <td class="tdHec">1.88E+05</td>
| + | |
| − | <td class="tdHec">8.83E+05</td>
| + | |
| − | <td class="tdHec">3.06E+05</td>
| + | |
| − | <td class="tdHec">3.54E+04</td>
| + | |
| − | <td class="tdHec">5.61E+05</td>
| + | |
| − | <td class="tdHec">1.98E+06</td>
| + | |
| − | <td class="tdHec">1.66E+05</td>
| + | |
| − |
| + | |
| − |
| + | |
| − | </tr>
| + | |
| − | </tbody></table>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
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| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <div id="loli" style="
| + | |
| − | padding-bottom: 1em;
| + | |
| − | text-align: right;
| + | |
| − | ">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2018/6/69/T--Valencia_UPV--MerceríaPaquitaUPV2018.jpeg" | + | |
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| − | <img src="https://static.igem.org/mediawiki/2018/6/69/T--Valencia_UPV--MerceríaPaquitaUPV2018.jpeg" alt="" style=" | + | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--Valencia_UPV--im1UPV2018.jpeg" alt="" style=" |
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| − | <img src="https://static.igem.org/mediawiki/2018/6/69/T--Valencia_UPV--MerceríaPaquitaUPV2018.jpeg" alt="" style=" | + | <img src="https://static.igem.org/mediawiki/2018/2/28/T--Valencia_UPV--im2UPV2018.jpeg" alt="" style=" |
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