Difference between revisions of "Team:Aalto-Helsinki/Demonstrate"

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<h1 align="center">Demonstrate</h1>
 
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      <p class="blue-text">Demonstration 1</p>
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    <h3 align="center">Cloning</h4>
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     <p>We assembled our constructs and cloned them into pSVA1 plasmid using Golden Gate assembly. After transforming plasmids to E.Coli  Beta-10 and plasmid isolation we performed a check-up digestion with restriction enzymes NcoI and XhoI which cut the plasmid backbone close to BsaI insert site.</p>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your project works. There are many ways in which your project working could be demonstrated, so there is more than one way to meet this requirement. This gold medal criterion was introduced in 2016, so check our what 2016 teams did to achieve their gold medals!
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Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
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    <h3 align="center">Protein expression and purification</h3>
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    <p>We were able to successfully purify our silk containing construct prancerPurple-silk-CBM with the heat protocol</p>
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    <h3 align="center">Cellulose binding </h3>
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h3>What should this page contain?</h3>
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<li> Clearly and objectively describe the results of your work.</li>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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<h3>Describe what your results mean </h3>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
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</ul>
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<h3> Project Achievements </h3>
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<li>A list of linked bullet points of the successful results during your project</li>
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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<h3>Inspiration</h3>
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<p>See how other teams presented their results.</p>
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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Revision as of 17:44, 11 October 2018


Demonstrate


Cloning

We assembled our constructs and cloned them into pSVA1 plasmid using Golden Gate assembly. After transforming plasmids to E.Coli Beta-10 and plasmid isolation we performed a check-up digestion with restriction enzymes NcoI and XhoI which cut the plasmid backbone close to BsaI insert site.


Protein expression and purification

We were able to successfully purify our silk containing construct prancerPurple-silk-CBM with the heat protocol


Cellulose binding

Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.


Cellulose nanofibril binding

Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.


Cotton binding

Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.


Binding to hair keratin

Eget duis at tellus at urna. Lacinia quis vel eros donec. Ut pharetra sit amet aliquam id diam. Erat imperdiet sed euismod nisi porta.






Results

Here you can describe the results of your project and your future plans.

What should this page contain?

  • Clearly and objectively describe the results of your work.
  • Future plans for the project.
  • Considerations for replicating the experiments.

Describe what your results mean

  • Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
  • Show data, but remember all measurement and characterization data must be on part pages in the Registry.
  • Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.

Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

  • A list of linked bullet points of the successful results during your project
  • A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.

Inspiration

See how other teams presented their results.