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{{Tacoma_RAINmakers}} | {{Tacoma_RAINmakers}} | ||
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+ | /*Length of the text box in the submenu */ | ||
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+ | margin-right: auto } | ||
+ | |||
+ | h1{ | ||
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+ | <body> | ||
+ | <div id="topbox"> | ||
+ | <h1>Tacoma RAINmakers Design</h1> | ||
+ | </div> | ||
+ | |||
+ | <div class="container"> | ||
+ | <h1>The circuit design</h1> | ||
+ | <!-- | ||
+ | <h1>Gel Extraction</h1> <h2></h2> | ||
+ | <p>1.After taking a picture of the results of the gel electrophoresis, use blue light along with an orange filter to help find the DNA bands.<BR> | ||
+ | 2. Place a cut right underneath the band of DNA that is intended for extraction.<BR> | ||
+ | 3. Cut a piece of filter paper and dialysis tubing to an appropriate size so that it would fit inside the slit.<BR> | ||
+ | 4. Stick the tubing and filter paper together. Place this into the slit so that the side with the filter paper faces the band of DNA.<BR> | ||
+ | 5. Place the gel in a gel box. Run the gel at 120V for 10 minutes or until the DNA moves into the filter paper and stays. <BR> | ||
+ | 6. After running the gel, place the casting tray under blue light once again to ensure that the DNA has successfully moved onto the filter paper. <BR> | ||
+ | 7. Take a 0.5mL centrifuge tube and pierce a small hole at the bottom of the tube. Place this small tube in a 1.5mL centrifuge tube. <BR> | ||
+ | 8. Place the filter paper with the DNA into the small centrifuge tube. Centrifuge both tubes. This allows the supernatant to flow out of the small tube into the large one. <BR> | ||
+ | 9. Take the small centrifuge tube out and discard it. The DNA has been extracted.<BR> </p> | ||
+ | --> | ||
+ | </div> | ||
+ | </body> | ||
+ | |||
+ | </div> <div class="visualClear"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
</html> | </html> |
Latest revision as of 01:45, 12 October 2018