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          <h1>Gel Extraction</h1> <h2></h2>
 
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<p>1.After taking a picture of the results of the gel electrophoresis, use blue light along with an orange filter to help find the DNA bands.<BR>
 
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2. Place a cut right underneath the band of DNA that is intended for extraction.<BR>
 
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3. Cut a piece of filter paper and dialysis tubing to an appropriate size so that it would fit inside the slit.<BR>
 
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4. Stick the tubing and filter paper together. Place this into the slit so that the side with the filter paper faces the band of DNA.<BR>
 
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5. Place the gel in a gel box. Run the gel at 120V for 10 minutes or until the DNA moves into the filter paper and stays. <BR>
 
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6. After running the gel, place the casting tray under blue light once again to ensure that the DNA has successfully moved onto the filter paper. <BR>
 
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7. Take a 0.5mL centrifuge tube and pierce a small hole at the bottom of the tube. Place this small tube in a 1.5mL centrifuge tube. <BR>
 
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8. Place the filter paper with the DNA into the small centrifuge tube. Centrifuge both tubes. This allows the supernatant to flow out of the small tube into the large one. <BR>
 
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9. Take the small centrifuge tube out and discard it. The DNA has been extracted.<BR> </p>
 
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Latest revision as of 01:51, 12 October 2018

Team:TacomaRAINmakers/Notebook - 2017.igem.org

Team:ECUST/Lab/Notebook


Team- 2018.igem.org

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Team:RAINmakers/Notebook

Tacoma RAINmakers Results

The Results




Here you can describe the results of your project and your future plans.


What should this page contain?

  • Clearly and objectively describe the results of your work.
  • Future plans for the project.
  • Considerations for replicating the experiments.



Describe what your results mean

  • Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for.
  • Show data, but remember all measurement and characterization data must be on part pages in the Registry.
  • Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project.



Project Achievements

You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.

  • A list of linked bullet points of the successful results during your project
  • A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.