Difference between revisions of "Team:Jiangnan China/Collaborations"

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{{Jiangnan_China}}
 
{{Jiangnan_China}}
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<html>
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<html lang="en">
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  <body style="background-color: #ccc">
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          <img src="https://static.igem.org/mediawiki/2018/8/84/T--jiangnan_china--home--icon-logo.png" width="36px" height="36px">
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        </a>
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        <a class="nav-link py-2 d-none d-md-inline-block" href="#"><i class="fa fa-home"></i> Home</a>
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            <i class="fa fa-group"></i> Team
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Description">Description</a>
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            <i class="fa fa-composer"></i> Human Pactices
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            <i class="fa fa-trophy"></i> Awards
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Bronzes">Bronzes</a>
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            <i class="fa fa-part"></i> Parts
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          </a>
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          <div class="dropdown-menu" aria-labelledby="partsDropdown">
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/BasicParts">Basic Parts</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Compositeparts">Composite parts</a>
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          </div>
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        </div>
  
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        <a class="nav-link py-2 d-none d-md-inline-block" href="https://2018.igem.org/Team:Jiangnan_China/InterLab"><i class="fa fa-interlab" aria-hidden="true"></i> InterLab</a>
  
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      </div>
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    </nav>
  
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<div class="column full_size judges-will-not-evaluate">
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    <main class="content-wrap">
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<h3>★  ALERT! </h3>
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      <img src="https://static.igem.org/mediawiki/2018/b/b2/T--jiangnan_china--interlab--1.jpg" width="100%">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Overview</strong></div>
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</div>
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    <br>
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      &nbsp;&nbsp;&nbsp;&nbsp;In the last 4 years, the Measurement Committee has been developing a robust measurement procedure for green fluorescent protein (GFP) though the Interlab study, aiming to improve the measurement tools available to both the iGEM community and the synthetic biology community as a whole. And this year, we are delighted to join the study to know if we can reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD.
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      <br><br>
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      </div>
  
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      <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Materials</strong></div>
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    <br>
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      96-spot well plates: clear plates from Corning<br>
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      Bacteria strains: E.coli DH5α<br>
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      Test devices: obtained from the distribution kit
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      <br><br>
  
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<div class="clear"></div>
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      <table class="table" id="HQ_page">
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        <thead>
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          <tr><th>Device</th>
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          <th>Part number</th>
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          <th>Location</th>
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        </tr></thead>
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        <tbody>
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          <tr>
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            <td><a text-decoration="underline">Position control </a></td>
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            <td>BBa_R0040</td>
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            <td>Well 2D</td>
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          </tr>
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 +
          <tr>
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            <td><a text-decoration="underline">Negative control</a></td>
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            <td>BBa_I20270</td>
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            <td>Well 2B</td>
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          </tr>
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                <tr>
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            <td><a text-decoration="underline">Test Device 1</a></td>
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            <td>BBa_J364000</td>
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            <td>Well 2F</td>
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          </tr>
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          <tr>
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            <td><a text-decoration="underline">Test Device 2</a></td>
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            <td>BBa_J364001</td>
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            <td>Well 2H</td>
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          </tr>
 +
          <tr>
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            <td><a text-decoration="underline">Test Device 3</a></td>
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            <td>BBa_J364002</td>
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            <td>Well 2J</td>
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          </tr>
 +
          <tr>
 +
            <td><a text-decoration="underline">Test Device 4</a></td>
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            <td>BBa_J364007</td>
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            <td>Well 2L</td>
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          </tr>
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          <tr>
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            <td><a text-decoration="underline">Test Device 5</a></td>
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            <td>BBa_J364008</td>
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            <td>Well 2N</td>
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          </tr>
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          <tr>
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            <td><a text-decoration="underline">Test Device 6</a></td>
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            <td>BBa_J364009</td>
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            <td>Well 2P</td>
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          </tr>
 +
        </tbody>
 +
      </table>
 +
      </div>
  
 +
      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Protocols</strong></div>
 +
      <br><br>
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      <p style="text-align: center;"><a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf" class="btn btn-info">Here is the link </a></p>
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      </div>
  
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<div class="column full_size">
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      <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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<h1>Collaborations</h1>
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Results</strong></div>
 +
      <br><br>
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    <div align="center" ><strong><font size="5" color="#5B9BD5" >Particle Standard Curve</font></strong></div>
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    <br>
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    <div align="center">
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      <img src="https://static.igem.org/mediawiki/2018/7/77/T--jiangnan_china--interlab--chart1.png" width="70%" >
 +
    </div>
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    <br><br>
  
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<p>
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Particle Standard Curve (log scale)</font></strong></div>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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    <br>
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</p>
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    <div align="center">
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      <img src="https://static.igem.org/mediawiki/2018/4/4b/T--jiangnan_china--interlab--chart2.png" width="70%" >
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    </div>
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    <br><br>
  
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<h3>Silver Medal Criterion #2</h3>
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Fluorescein Standard Curve</font></strong></div>
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<p>
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    <br>
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Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.  
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    <div align="center">
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</p>
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      <img src="https://static.igem.org/mediawiki/2018/3/31/T--jiangnan_china--interlab--chart3.png" width="70%" >
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</div>
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    </div>
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    <br><br>
  
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<div class="column two_thirds_size">
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Fluorescein Standard Curve (log scale)</font></strong></div>
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    <br>
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    <div align="center">
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      <img src="https://static.igem.org/mediawiki/2018/f/f9/T--jiangnan_china--interlab--chart4.png" width="70%" >
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    </div>
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    <br><br>
  
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<h4> Which other teams can we work with? </h4>
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    <div align="center"><strong><font size="5" color="#5B9BD5" >uM Fluorescein / OD</font></strong></div>
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<p>  
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    <br>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
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    <div align="center">
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</p>
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      <img src="https://static.igem.org/mediawiki/2018/5/55/T--jiangnan_china--interlab--chart5.png" width="70%" >
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    </div>
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    <br><br>
  
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<p>
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Net Fluorescein a.u.</font></strong></div>
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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    <br>
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</p>
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    <div align="center">
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      <img src="https://static.igem.org/mediawiki/2018/a/a1/T--jiangnan_china--interlab--chart6.png" width="70%" >
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    </div>
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    <br><br>
  
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</div>
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Net Abs 600</font></strong></div>
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    <br>
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    <div align="center">
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      <img src="https://static.igem.org/mediawiki/2018/d/d0/T--jiangnan_china--interlab--chart7.png" width="70%" >
 +
    </div>
 +
    <br><br>
  
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    <div align="center"><strong><font size="5" color="#5B9BD5" >MEFL / particle</font></strong></div>
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    <br>
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    <div align="center">
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      <img src="https://static.igem.org/mediawiki/2018/b/b0/T--jiangnan_china--interlab--chart8.png" width="70%" >
 +
    </div>
 +
    <br><br>
  
 +
      </div>
  
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<div class="column third_size">
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      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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<p>
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Summery</strong></div>
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Here are some suggestions for projects you could work on with other teams:
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    <br>
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</p>
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      &nbsp;&nbsp;&nbsp;&nbsp;We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.
 +
      <br><br>
 +
      </div>
 +
      <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
 +
    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Conclution</strong></div>
 +
    <br>
 +
      <div>
 +
      &nbsp;&nbsp;&nbsp;&nbsp;We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.
 +
      </div>
 +
      <br>
 +
      <div>
 +
      <font size="5">1.</font> The final concentration of chloramphenicol is not clearly defined in the experimental protocol. Although we have made the experiment proceed smoothly based on experience, we believe that accurate antibiotic dosage requirements are still necessary;
 +
      </div>
 +
      <br>
 +
      <div>
 +
      <font size="5">2.</font> Regarding the use of the plate reader: although there are some descriptions in the experimental protocol that explain the experimental design and a simple explanation of the plate reader, we used the plate reader incorrectly at the beginning of the experiment, leading to a invalid data. So we still expect more instructions on the setup and use of the plate reader;
 +
      </div>
 +
      <br>
 +
      <div>
 +
      <font size="5">3.</font> Regarding the preservation of competent cells: we have transferred the competent cells position between buildings due to the change of the laboratory position, which took about 10 minutes. And in the transformation experiment, the competent cells stayed outside for too long. These improper storage of competent cells made the bacteria grow very unsatisfactorily after plating. Therefore, the preservation of competent cells is very important.
 +
      </div>
 +
      <br><br>
 +
      </div>
  
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<ul>
+
     
−
<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulate another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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</ul>
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</div>
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 +
 +
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 +
 +
    <footer class="py-4">
 +
      <div class="container" style="text-align: center;">
 +
        <a href="http://www.jiangnan.edu.cn/" title="江南大学"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--jiangnan_china--jiangnanlogo.png"></a>
 +
        <a href="http://biotech.jiangnan.edu.cn/" title="江南大学生物工程学院"><img src="https://static.igem.org/mediawiki/2018/a/a6/T--jiangnan_china--shenggonglogo.png"></a>
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Revision as of 05:14, 12 October 2018

Overview

    In the last 4 years, the Measurement Committee has been developing a robust measurement procedure for green fluorescent protein (GFP) though the Interlab study, aiming to improve the measurement tools available to both the iGEM community and the synthetic biology community as a whole. And this year, we are delighted to join the study to know if we can reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD.

Materials

96-spot well plates: clear plates from Corning
Bacteria strains: E.coli DH5α
Test devices: obtained from the distribution kit

Device Part number Location
Position control BBa_R0040 Well 2D
Negative control BBa_I20270 Well 2B
Test Device 1 BBa_J364000 Well 2F
Test Device 2 BBa_J364001 Well 2H
Test Device 3 BBa_J364002 Well 2J
Test Device 4 BBa_J364007 Well 2L
Test Device 5 BBa_J364008 Well 2N
Test Device 6 BBa_J364009 Well 2P
Protocols


Here is the link

Results


Particle Standard Curve



Particle Standard Curve (log scale)



Fluorescein Standard Curve



Fluorescein Standard Curve (log scale)



uM Fluorescein / OD



Net Fluorescein a.u.



Net Abs 600



MEFL / particle



Summery

    We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.

Conclution

    We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.

1. The final concentration of chloramphenicol is not clearly defined in the experimental protocol. Although we have made the experiment proceed smoothly based on experience, we believe that accurate antibiotic dosage requirements are still necessary;

2. Regarding the use of the plate reader: although there are some descriptions in the experimental protocol that explain the experimental design and a simple explanation of the plate reader, we used the plate reader incorrectly at the beginning of the experiment, leading to a invalid data. So we still expect more instructions on the setup and use of the plate reader;

3. Regarding the preservation of competent cells: we have transferred the competent cells position between buildings due to the change of the laboratory position, which took about 10 minutes. And in the transformation experiment, the competent cells stayed outside for too long. These improper storage of competent cells made the bacteria grow very unsatisfactorily after plating. Therefore, the preservation of competent cells is very important.


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