Difference between revisions of "Team:Jiangnan China/Collaborations"

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             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Results">Results</a>
 
             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Results">Results</a>
 
             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Demonstrate">Demonstrate</a>
 
             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Demonstrate">Demonstrate</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Model">Model</a>
 
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             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Overview">Silver_HP</a>
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             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Human_Practices">Silver_HP</a>
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             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Socialresearch">Gold_HP</a>
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             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Human_Practices">Gold_HP</a>
 
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     <main class="content-wrap">
 
     <main class="content-wrap">
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       <img src="https://static.igem.org/mediawiki/2018/b/b2/T--jiangnan_china--interlab--1.jpg" width="100%">
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       <img src="https://static.igem.org/mediawiki/2018/c/c5/T--jiangnan_china--collaborations--1.jpg" width="100%">
 
       <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
 
       <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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     <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Overview</strong></div>
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     <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong><font size="6" color="#ED7D31">Interlab with ZJUT-China</font></strong></div>
 
     <br>
 
     <br>
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       &nbsp;&nbsp;&nbsp;&nbsp;In the last 4 years, the Measurement Committee has been developing a robust measurement procedure for green fluorescent protein (GFP) though the Interlab study, aiming to improve the measurement tools available to both the iGEM community and the synthetic biology community as a whole. And this year, we are delighted to join the study to know if we can reduce lab-to-lab variability in fluorescence measurements by normalizing to absolute cell count or colony-forming units (CFUs) instead of OD.
+
       &nbsp;&nbsp;&nbsp;&nbsp;This year is our first time to attend for the Igem, So We met up with ZJUT-China，DLUT-China and LZU-China in Jiangsu Normal University to learn some experience form them and share our own project to them . Before this meeting, we had some troubles in interlab and we couldn’t find correct method to solve it. So we shared our problems with them at the meeting. The ZJUT-China had finished the interlab successfully and gave us some suggestions on our interlab. We adopted their suggestions and finally made it. After this meeting, we had a further communication with ZJUT-China and What they studied in Lysis gene gave us some inspiration about our own project, which made us consider to add a kill switch to make our L.lactis safer towards the biological environment. In return, we also gave them some ideas and suggestions to help them with their project.
 
       <br><br>
 
       <br><br>
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      <div class="row" style="margin-top:20px;">
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    <div class="col-md-6">
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      <div align="center" style="padding-right:85px"><img src="img/zjutlogo.png" width="65%"></div>
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    </div>
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        <div class="col-md-6">
 +
      <div align="center" style="padding-right:85px"><img src="img/zjut.jpg" width="100%"></div>
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      </div>
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  </div>
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    <br>
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       </div>
 
       </div>
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 +
     
  
 
       <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
 
       <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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     <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Materials</strong></div>
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     <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong><font size="6" color="#ED7D31">Kill switch with SSTi-SGZD</font></strong></div>
 
     <br>
 
     <br>
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       96-spot well plates: clear plates from Corning<br>
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       &nbsp;&nbsp;&nbsp;&nbsp;We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.
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      Bacteria strains: E.coli DH5α<br>
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      Test devices: obtained from the distribution kit
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       <br><br>
 
       <br><br>
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      <table class="table" id="HQ_page">
 
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        <thead>
 
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          <tr><th>Device</th>
 
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          <th>Part number</th>
 
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          <th>Location</th>
 
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        </tr></thead>
 
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        <tbody>
 
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          <tr>
 
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            <td><a text-decoration="underline">Position control </a></td>
 
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            <td>BBa_R0040</td>
 
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            <td>Well 2D</td>
 
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          </tr>
 
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          <tr>
 
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            <td><a text-decoration="underline">Negative control</a></td>
 
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            <td>BBa_I20270</td>
 
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            <td>Well 2B</td>
 
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          </tr>
 
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                <tr>
 
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            <td><a text-decoration="underline">Test Device 1</a></td>
 
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            <td>BBa_J364000</td>
 
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            <td>Well 2F</td>
 
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          </tr>
 
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          <tr>
 
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            <td><a text-decoration="underline">Test Device 2</a></td>
 
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            <td>BBa_J364001</td>
 
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            <td>Well 2H</td>
 
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          </tr>
 
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          <tr>
 
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            <td><a text-decoration="underline">Test Device 3</a></td>
 
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            <td>BBa_J364002</td>
 
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            <td>Well 2J</td>
 
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          </tr>
 
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          <tr>
 
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            <td><a text-decoration="underline">Test Device 4</a></td>
 
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            <td>BBa_J364007</td>
 
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            <td>Well 2L</td>
 
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          </tr>
 
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          <tr>
 
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            <td><a text-decoration="underline">Test Device 5</a></td>
 
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            <td>BBa_J364008</td>
 
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            <td>Well 2N</td>
 
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          </tr>
 
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          <tr>
 
−
            <td><a text-decoration="underline">Test Device 6</a></td>
 
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            <td>BBa_J364009</td>
 
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            <td>Well 2P</td>
 
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          </tr>
 
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        </tbody>
 
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      </table>
 
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      </div>
 
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      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
 
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Protocols</strong></div>
 
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      <br><br>
 
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      <p style="text-align: center;"><a href="https://static.igem.org/mediawiki/2018/0/09/2018_InterLab_Plate_Reader_Protocol.pdf" class="btn btn-info">Here is the link </a></p>
 
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      </div>
 
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      <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
 
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Results</strong></div>
 
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      <br><br>
 
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    <div align="center" ><strong><font size="5" color="#5B9BD5" >Particle Standard Curve</font></strong></div>
 
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    <br>
 
 
     <div align="center">
 
     <div align="center">
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       <img src="https://static.igem.org/mediawiki/2018/7/77/T--jiangnan_china--interlab--chart1.png" width="70%" >
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       <img src="img/ssti.jpg" width="20%" >
 
     </div>
 
     </div>
 
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     <br><br>
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Particle Standard Curve (log scale)</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/4/4b/T--jiangnan_china--interlab--chart2.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Fluorescein Standard Curve</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/3/31/T--jiangnan_china--interlab--chart3.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Fluorescein Standard Curve (log scale)</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/f/f9/T--jiangnan_china--interlab--chart4.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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    <div align="center"><strong><font size="5" color="#5B9BD5" >uM Fluorescein / OD</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/5/55/T--jiangnan_china--interlab--chart5.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Net Fluorescein a.u.</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/a/a1/T--jiangnan_china--interlab--chart6.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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    <div align="center"><strong><font size="5" color="#5B9BD5" >Net Abs 600</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/d/d0/T--jiangnan_china--interlab--chart7.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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    <div align="center"><strong><font size="5" color="#5B9BD5" >MEFL / particle</font></strong></div>
 
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    <br>
 
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    <div align="center">
 
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      <img src="https://static.igem.org/mediawiki/2018/b/b0/T--jiangnan_china--interlab--chart8.png" width="70%" >
 
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    </div>
 
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    <br><br>
 
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       </div>
 
       </div>
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       <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
 
       <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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     <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Summery</strong></div>
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     <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong><font size="6" color="#ED7D31">Satety with  Unesp_Brazilr</font></strong></div>
 
     <br>
 
     <br>
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       &nbsp;&nbsp;&nbsp;&nbsp;We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.
+
       &nbsp;&nbsp;&nbsp;&nbsp;This year we also contacted with the team Unesp_Brazilr by e-mails. We all concerned about the safety of the synthetic biology so we talked more about how to construct a high efficient chassis microorganism which is safe enough. Although we wanted to make our strain applied into the practical application, we didn’t know anything about the application of engineering bacteria. Fortunately, Unesp_Brazil offered the regulations and instructed us to apply our bacteria in the correct way. They also offered us the gene which could be added as the kill switch, helping us to notice the importance of using a kill switch to make our bacteria safer. Of course, we give some methods to help with their experiments and shared some experience about our project. Because this is the first time to attend for the IGEM, we are very appreciated that Unesp_Brazil could share experience with us and adopted our ideas even though we are a new team for IGEM.
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      <br><br>
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      </div>
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      <div id="dcpt4" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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    <div align="left" style="font-family: 'spr';font-size:40px;border-bottom:2px solid #584b4f;"><strong>Conclution</strong></div>
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−
    <br>
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−
      <div>
+
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      &nbsp;&nbsp;&nbsp;&nbsp;We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.
+
−
      </div>
+
−
      <br>
+
−
      <div>
+
−
      <font size="5">1.</font> The final concentration of chloramphenicol is not clearly defined in the experimental protocol. Although we have made the experiment proceed smoothly based on experience, we believe that accurate antibiotic dosage requirements are still necessary;
+
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      </div>
+
−
      <br>
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−
      <div>
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−
      <font size="5">2.</font> Regarding the use of the plate reader: although there are some descriptions in the experimental protocol that explain the experimental design and a simple explanation of the plate reader, we used the plate reader incorrectly at the beginning of the experiment, leading to a invalid data. So we still expect more instructions on the setup and use of the plate reader;
+
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      </div>
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−
      <br>
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−
      <div>
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−
      <font size="5">3.</font> Regarding the preservation of competent cells: we have transferred the competent cells position between buildings due to the change of the laboratory position, which took about 10 minutes. And in the transformation experiment, the competent cells stayed outside for too long. These improper storage of competent cells made the bacteria grow very unsatisfactorily after plating. Therefore, the preservation of competent cells is very important.
+
−
      </div>
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       <br><br>
 
       <br><br>
 +
      <div align="center">
 +
      <img src="img/unes.png" width="20%" >
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Revision as of 07:12, 12 October 2018

Interlab with ZJUT-China

    This year is our first time to attend for the Igem, So We met up with ZJUT-China，DLUT-China and LZU-China in Jiangsu Normal University to learn some experience form them and share our own project to them . Before this meeting, we had some troubles in interlab and we couldn’t find correct method to solve it. So we shared our problems with them at the meeting. The ZJUT-China had finished the interlab successfully and gave us some suggestions on our interlab. We adopted their suggestions and finally made it. After this meeting, we had a further communication with ZJUT-China and What they studied in Lysis gene gave us some inspiration about our own project, which made us consider to add a kill switch to make our L.lactis safer towards the biological environment. In return, we also gave them some ideas and suggestions to help them with their project.


Kill switch with SSTi-SGZD

    We studied for a time before doing interlab, and spent one-two day preparing experimental materials, such as preparing E. coli DH5α and several culture media. After full preparation, we started the experiment. In the first experiment, our antibiotics failed, causing too much bacteria to colonize the plate.The second time we had a problem with the competent cells, so the bacteria did not grow after the plate was painted.The third time, we reprepared the competent cells and replaced the antibiotics, and the experiment went well.



Satety with Unesp_Brazilr

    This year we also contacted with the team Unesp_Brazilr by e-mails. We all concerned about the safety of the synthetic biology so we talked more about how to construct a high efficient chassis microorganism which is safe enough. Although we wanted to make our strain applied into the practical application, we didn’t know anything about the application of engineering bacteria. Fortunately, Unesp_Brazil offered the regulations and instructed us to apply our bacteria in the correct way. They also offered us the gene which could be added as the kill switch, helping us to notice the importance of using a kill switch to make our bacteria safer. Of course, we give some methods to help with their experiments and shared some experience about our project. Because this is the first time to attend for the IGEM, we are very appreciated that Unesp_Brazil could share experience with us and adopted our ideas even though we are a new team for IGEM.



Copyright © jiangnan_China 2018