Difference between revisions of "Team:BGU Israel/Notebook"
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<p>We began working in our brand-new lab space! When we first arrived, the lab was nothing but two work benches. We had to collect and organize the equipment, materials, cell culture room, and work area; all according to lab safety standards. We also began cloning the plasmids for our experiments and learning how to work with cell culture by practicing on HEK293 cells.<br/> | <p>We began working in our brand-new lab space! When we first arrived, the lab was nothing but two work benches. We had to collect and organize the equipment, materials, cell culture room, and work area; all according to lab safety standards. We also began cloning the plasmids for our experiments and learning how to work with cell culture by practicing on HEK293 cells.<br/> | ||
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<b>Highlights:</b> clone Steap4, Timp1, F4/80 into pGL4.17 and pGL3 plasmid for Promoter Assay. | <b>Highlights:</b> clone Steap4, Timp1, F4/80 into pGL4.17 and pGL3 plasmid for Promoter Assay. | ||
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| − | In March, we experienced our first little success. We cloned our promoters into the pGL3 vector and were able to begin our first experiment, the Promoter Assay. Meanwhile, so far, our practice cells were alive and well.<br/> | + | In March, we experienced our first little success. We cloned our promoters into the pGL3 vector and were able to begin our first experiment, the Promoter Assay. Meanwhile, so far, our practice cells were alive and well. |
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<b>Highlights:</b> A decision was made to concentrate our efforts on the pGL3 plasmid expression assay. Because, then we could compare the expression among the promoters and we wouldn’t have to use two sets of luciferase kits. | <b>Highlights:</b> A decision was made to concentrate our efforts on the pGL3 plasmid expression assay. Because, then we could compare the expression among the promoters and we wouldn’t have to use two sets of luciferase kits. | ||
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Revision as of 17:32, 12 October 2018
Notebook
February
We began working in our brand-new lab space! When we first arrived, the lab was nothing but two work benches. We had to collect and organize the equipment, materials, cell culture room, and work area; all according to lab safety standards. We also began cloning the plasmids for our experiments and learning how to work with cell culture by practicing on HEK293 cells.
Highlights: clone Steap4, Timp1, F4/80 into pGL4.17 and pGL3 plasmid for Promoter Assay.
March
In March, we experienced our first little success. We cloned our promoters into the pGL3 vector and were able to begin our first experiment, the Promoter Assay. Meanwhile, so far, our practice cells were alive and well.
Highlights: A decision was made to concentrate our efforts on the pGL3 plasmid expression assay. Because, then we could compare the expression among the promoters and we wouldn’t have to use two sets of luciferase kits.
April
In this example we have added a "plus" sign to each button. When the user clicks on the button, the "plus" sign is replaced with a "minus" sign.
May
In this example we have added a "plus" sign to each button. When the user clicks on the button, the "plus" sign is replaced with a "minus" sign.
June
In this example we have added a "plus" sign to each button. When the user clicks on the button, the "plus" sign is replaced with a "minus" sign.
July
In this example we have added a "plus" sign to each button. When the user clicks on the button, the "plus" sign is replaced with a "minus" sign.
August
In this example we have added a "plus" sign to each button. When the user clicks on the button, the "plus" sign is replaced with a "minus" sign.
Experiments
In this example we have added a "plus" sign to each button. When the user clicks on the button, the "plus" sign is replaced with a "minus" sign.